Calcitriol ELISA Kit for General() | EIAab.comCalcitriol ELISA Kit for General()

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Calcitriol (Shorten name),

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General Annotation

Predicted Eptitope

Vaild Sequence

Citations (3)

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Shorten name:

Calcitriol;

Antigen name:

Calcitriol;

Alternative:

1,25-dihydroxyvitamin D3;1,25-dihydroxycholecalciferol;

General Annotation

Sub Unit:

Function:

Calcitriol, also called 1,25-dihydroxycholecalciferol or 1,25-dihydroxyvitamin D3, is the hormonally active form of vitamin D with three hydroxyl groups (abbreviated 1,25-(OH)2D3 or simply 1,25(OH)2D). It increases the level of calcium (Ca2+) in the blood by (1) increasing the uptake of calcium from the gut into the blood, (2) decreasing the transfer of calcium from blood to the urine by the kidney, and (3) increasing the release of calcium into the blood from bone.

Location:

Calcitriol is produced in the cells of the proximal tubule of the nephron in the kidneys by the action of 25-hydroxyvitamin D3 1-alpha-hydroxylase, a mitochondrial oxygenase and an enzyme which catalyzes the hydroxylation of 25-hydroxycholecalciferol (calcifediol). The activity of the enzyme is stimulated by PTH. The reaction is an important control point in Ca2+ homeostasis. The production of calcitriol is also increased by prolactin, a hormone which stimulates lactogenesis (the formation of breast milk), a process which requires large amounts of calcium. It is decreased by high levels of serum phosphate and by an increase in the production of the hormone FGF-23 by osteocyte cells in bone.

Antigen Attributes:

Formula:

C27H44O3

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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FOR

ELISA Kit for General Calcitriol

Cat.:

E0467Ge

Price:

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Manual:

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MSDS:

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Packing:

96T

Range:

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CLIA Kit for General Calcitriol

Cat.:

U0467Ge

Price:

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Manual:

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MSDS:

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Packing:

96T

Range:

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R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.7

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.6%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of Calcitriol into serum and plasma .

Sample Type	Average(%)	Recovery Range (%)
Serum	90	88-98
Plasma	84	79-93

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Calcitriol and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	80-91%	86-95%	78-90%	89-97%
EDTA plasma(n=5)	85-93%	87-96%	82-90%	78-93%
heparin plasma(n=5)	88-93%	86-92%	83-94%	85-97%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Citations

[1].

"Phosphate overload directly induces systemic inflammation and malnutrition as well as vascular calcification in uremia"

S Yamada, M Tokumoto, N Tatsumoto, et al.

From:American journal of physiology. Renal physiology

[2].

"Impact of Fibroblast Growth Factor-23 on Peripheral Arterial Disease in Type 2 Diabetes Mellitus: A Comparative Cross-Sectional Pilot Study"

E Hatipoğlu, N Özgür, M Niyazoğlu, et al.

From:Turkish Journal of Endocrinology and Metabolism

[3].

"Thiazide-Sensitive Na+-Cl- Cotransporter (NCC) Gene Inactivation Results in Increased Duodenal Ca2+ Absorption, Enhanced Osteoblast Differentiation..."

Hsu Y J, Yang S S, Cheng C J, et al.

From:Journal of Bone and Mineral Research

References

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