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Index > Protein center > ADIPOQ(Gene name) > Human
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  • ADIPOQ (Gene name),
  • Adiponectin (Protein name ),  ADIPO_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Related Databases
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  • Gene name:
    ADIPOQ(ACDC;ACRP30;APM1;GBP28);
    Protein name:
    Adiponectin;
    Alternative:
    Adipocyte complement-related 30 kDa protein(ACRP30);30 kDa adipocyte complement-related protein;Adipose most abundant gene transcript 1 protein(apM-1);Adipocyte, C1q and collagen domain-containing protein;Gelatin-binding protein;
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Homomultimer. Forms trimers, hexamers and 12- to 18-mers. The trimers (low molecular weight complexes / LMW) are assembled via non-covalent interactions of the collagen-like domains in a triple helix and hydrophobic interactions within the globular C1q domain. Several trimers can associate to form disulfide-linked hexamers (middle molecular weight complexes / MMW) and larger complexes (higher molecular weight / HMW). The HMW-complex assembly may rely aditionally on lysine hydroxylation and glycosylation. LMW, MMW and HMW complexes bind to HBEGF, MMW and HMW complexes bind to PDGFB, and HMW complex binds to FGF2. Interacts with CTRP9A via the C1q domain (heterotrimeric complex).
    Function:
    Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
    Subcellular Location:
    Secreted
    Protein Attributes:
    Sequence length:
    244
    Sequence:
    50:
    MLLLGAVLLL | LALPGHDQET | TTQGPGVLLP | LPKGACTGWM | AGIPGHPGHN | 
    100:
    GAPGRDGRDG | TPGEKGEKGD | PGLIGPKGDI | GETGVPGAEG | PRGFPGIQGR | 
    150:
    KGEPGEGAYV | YRSAFSVGLE | TYVTIPNMPI | RFTKIFYNQQ | NHYDGSTGKF | 
    200:
    HCNIPGLYYF | AYHITVYMKD | VKVSLFKKDK | AMLFTYDQYQ | ENNVDQASGS | 
    244:
    VLLHLEVGDQ | VWLQVYGEGE | RNGLYADNDN | DSTFTGFLLY | HDTN
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    UniGene:
    String:
    SMR:
    KEGG:
    Pfam:
    MIM:
    Uniprot:
     
    FOR
    ELISA Kit for Human Adiponectin
    Cat.:
    E0605h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Adiponectin
    Cat.:
    E0605m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Adiponectin
    Cat.:
    E0605d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Adiponectin
    Cat.:
    E0605b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Adiponectin
    Cat.:
    E0605r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human Adiponectin
    Cat.:
    U0605b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human Adiponectin
    Cat.:
    U0605d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human Adiponectin
    Cat.:
    U0605r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Adiponectin
    Cat.:
    U0605h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Adiponectin
    Cat.:
    U0605m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Human Adiponectin
    Cat.:
    P0605Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Adiponectin
    Polyclonal Antibody for Human Adiponectin
    Cat.:
    P0605Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Adiponectin
    Polyclonal Antibody for Human Adiponectin
    Cat.:
    P0605Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Human Adiponectin
    Monoclonal Antibody for Human Adiponectin
    Monoclonal Antibody for Human Adiponectin
    Monoclonal Antibody for Human Adiponectin
    Monoclonal Antibody for Human Adiponectin
    Protein for Human Adiponectin
    Protein for Human Adiponectin
    Protein for Human Adiponectin
    Protein for Human Adiponectin
    Protein for Human Adiponectin
    Cat.:
    R0605h
    Price:
    Please sign in first.
    Packing:
    1mg
    Purity:
    >90%

    R&D Technical Data
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               12% SDS-PAGE Analysis

     

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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤ 4.5%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤ 7.1%

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    Recovery
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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    95

    91-98

    Plasma

    97

    92-102

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    87-95%

    90-101%

    92-103%

    90-98%

    EDTA plasma(n=5)

    89-95%

    91-102%

    89-101%

    90-102%

    heparin plasma(n=5)

    90-98%

     

    92-105%

    96-101%

    89-99%

     

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    References
    1. 1.
      "cDNA cloning and expression of a novel adipose specific collagen-like factor, apM1 (AdiPose Most abundant Gene transcript 1)."
      Maeda K. , Okubo K. , Shimomura I. , Funahashi T. , Matsuzawa Y. , Matsubara K.
      Biochem. Biophys. Res. Commun.221:286-289(1996) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      tissue: Adipose tissue.
    2. 2.
      "Organization of the gene for gelatin-binding protein (GBP28)."
      Saito K. , Tobe T. , Minoshima S. , Asakawa S. , Sumiya J. , Yoda M. , Nakano Y. , Shimizu N. , Tomita M.
      Gene229:67-73(1999) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    3. 3.
      "The human apM-1, an adipocyte-specific gene linked to the family of TNF's and to genes expressed in activated T cells, is mapped to chromosome 1q21.3-q23, a susceptibility locus identified for familial combined hyperlipidemia (FCH)."
      Schaeffler A. , Orso E. , Palitzsch K.D. , Buechler C. , Drobnik W. , Fuerst A. , Schoelmerich J. , Schmitz G.
      Biochem. Biophys. Res. Commun.260:416-425(1999) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    4. 4.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
    5. 5.
      "Signal peptide prediction based on analysis of experimentally verified cleavage sites."
      Zhang Z. , Henzel W.J.
      Protein Sci.13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 19-33
    6. 6.
      "Isolation and characterization of GBP28, a novel gelatin-binding protein purified from human plasma."
      Nakano Y. , Tobe T. , Choi-Miura N.H. , Mazda T. , Tomita M.
      J. Biochem.120:803-812(1996) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF N-TERMINUS;PARTIAL PROTEIN SEQUENCE
    7. 7.
      "Adiponectin, a new member of the family of soluble defense collagens, negatively regulates the growth of myelomonocytic progenitors and the functions of macrophages."
      Yokota T. , Oritani K. , Takahashi I. , Ishikawa J. , Matsuyama A. , Ouchi N. , Kihara S. , Funahashi T. , Tenner A.J. , Tomiyama Y. , Matsuzawa Y.
      Blood96:1723-1732(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CHARACTERIZATION
    8. 8.
      "Adiponectin, an adipocyte-derived plasma protein, inhibits endothelial NF-kappaB signaling through a cAMP-dependent pathway."
      Ouchi N. , Kihara S. , Arita Y. , Okamoto Y. , Maeda K. , Kuriyama H. , Hotta K. , Nishida M. , Takahashi M. , Muraguchi M. , Ohmoto Y. , Nakamura T. , Yamashita S. , Funahashi T. , Matsuzawa Y.
      Circulation102:1296-1301(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CHARACTERIZATION
    9. 9.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    10. 10.
      "Impaired multimerization of human adiponectin mutants associated with diabetes. Molecular structure and multimer formation of adiponectin."
      Waki H. , Yamauchi T. , Kamon J. , Ito Y. , Uchida S. , Kita S. , Hara K. , Hada Y. , Vasseur F. , Froguel P. , Kimura S. , Nagai R. , Kadowaki T.
      J. Biol. Chem.278:40352-40363(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBUNIT;DISULFIDE BOND;MUTAGENESIS OF CYS-36;CHARACTERIZATION OF VARIANTS ARG-84; SER-90; CYS-112 AND THR-164
    11. 11.
      "Adiponectin multimerization is dependent on conserved lysines in the collagenous domain: evidence for regulation of multimerization by alterations in posttranslational modifications."
      Richards A.A. , Stephens T. , Charlton H.K. , Jones A. , Macdonald G.A. , Prins J.B. , Whitehead J.P.
      Mol. Endocrinol.20:1673-1687(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBUNIT;HYDROXYLATION AT PRO-44; PRO-47; PRO-53; LYS-65; LYS-68; PRO-71; PRO-76; LYS-77; PRO-91; PRO-95 AND LYS-101;GLYCOSYLATION AT LYS-65; LYS-68; LYS-77 AND LYS-101;DISULFIDE BOND;LACK OF HYDROXYLATION AT PRO-62; PRO-86 AND PRO-104;LACK OF GLYCOSYLATION AT ASN-230;MUTAGENESIS OF LYS-33; CYS-36; LYS-65; LYS-68; LYS-77 AND LYS-101;IDENTIFICATION BY MASS SPECTROMETRY
    12. 12.
      "Sialic acid modification of adiponectin is not required for multimerization or secretion but determines half-life in circulation."
      Richards A.A. , Colgrave M.L. , Zhang J. , Webster J. , Simpson F. , Preston E. , Wilks D. , Hoehn K.L. , Stephenson M. , Macdonald G.A. , Prins J.B. , Cooney G.J. , Xu A. , Whitehead J.P.
      Mol. Endocrinol.24:229-239(2010) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION AT THR-21 AND THR-22;SUBUNIT;IDENTIFICATION BY MASS SPECTROMETRY;MUTAGENESIS OF THR-20; THR-21 AND THR-22
    13. 13.
      "Crystal structure of a single-chain trimer of human adiponectin globular domain."
      Min X. , Lemon B. , Tang J. , Liu Q. , Zhang R. , Walker N. , Li Y. , Wang Z.
      FEBS Lett.586:912-917(2012) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 104-244;SUBUNIT
    14. 14.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT ADPND CYS-112
    15. 15.
      "Genetic variation in the gene encoding adiponectin is associated with an increased risk of type 2 diabetes in the Japanese population."
      Hara K. , Boutin P. , Mori Y. , Tobe K. , Dina C. , Yasuda K. , Yamauchi T. , Otabe S. , Okada T. , Eto K. , Kadowaki H. , Hagura R. , Akanuma Y. , Yazaki Y. , Nagai R. , Taniyama M. , Matsubara K. , Yoda M. , more...
      Diabetes51:536-540(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS ARG-84; MET-117; THR-164; SER-221 AND PRO-241
    16. 16.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ERRATUM
    17. 17.
      "Association of adiponectin mutation with type 2 diabetes: a candidate gene for the insulin resistance syndrome."
      Kondo H. , Shimomura I. , Matsukawa Y. , Kumada M. , Takahashi M. , Matsuda M. , Ouchi N. , Kihara S. , Kawamoto T. , Sumitsuji S. , Funahashi T. , Matsuzawa Y.
      Diabetes51:2325-2328(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS CYS-112; THR-164; SER-221 AND PRO-241;ASSOCIATION WITH LOW PLASMA ADIPONECTIN CONCENTRATION AND DIABETES MELLITUS TYPE 2
    18. 18.
      "Single-nucleotide polymorphism haplotypes in the both proximal promoter and exon 3 of the APM1 gene modulate adipocyte-secreted adiponectin hormone levels and contribute to the genetic risk for type 2 diabetes in French Caucasians."
      Vasseur F. , Helbecque N. , Dina C. , Lobbens S. , Delannoy V. , Gaget S. , Boutin P. , Vaxillaire M. , Lepretre F. , Dupont S. , Hara K. , Clement K. , Bihain B. , Kadowaki T. , Froguel P.
      Hum. Mol. Genet.11:2607-2614(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS ARG-84; SER-90 AND HIS-111
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