Akt1 ELISA Kit for Mouse(Mus musculus) | EIAab.comAkt1 ELISA Kit for Mouse(Mus musculus)

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Akt1 (Gene name),

RAC-alpha serine/threonine-protein kinase (Protein name ), AKT1_MOUSE from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

Akt1(Akt;Rac);

Protein name:

RAC-alpha serine/threonine-protein kinase;

Alternative:

Protein kinase B(PKB);AKT1 kinase;Proto-oncogene c-Akt;Protein kinase B alpha(PKB alpha);Thymoma viral proto-oncogene;RAC-PK-alpha;

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

Interacts with AGAP2 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN and THEM4. Interacts with AKTIP (By similarity). Interacts (via PH domain) with MTCP1, TCL1A AND TCL1B. Interacts with CDKN1B; the interaction phosphorylates CDKN1B promoting 14-3-3 binding and cell-cycle progression. Interacts with MAP3K5 and TRAF6 (By similarity). Interacts with GRB10; the interaction leads to GRB10 phosphorylation thus promoting YWHAE/14-3-3-binding. Interacts with TNK2. Interacts with CLK2 and PPP2R5B. Interacts with STK3/MST2 and STK4/MST1.

Function:

AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility; prohibitin (PHB), playing an important role in cell metabolism and proliferation; and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.

Subcellular Location:

Cytoplasm Nucleus Cell membrane Nucleus after activation by integrin-linked protein kinase 1 (ILK1) (By similarity). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus.

Protein Attributes:

Sequence length:

480

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

480:

PPDQDDSMEC | VDSERRPHFP | QFSYSASGTA |

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

SMR:

P31750

Pfam:

PF00169

String:

P31750

UniGene:

Mm.6645

KEGG:

mmu:11651

Uniprot:

P31750

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ELISA Kit for Mouse RAC-alpha serine/threonine-protein kinase

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E0382r

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ELISA Kit for Mouse RAC-alpha serine/threonine-protein kinase

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E0382h

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96T

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ELISA Kit for Mouse RAC-alpha serine/threonine-protein kinase

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E0382m

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ELISA Kit for Mouse RAC-alpha serine/threonine-protein kinase

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E0382b

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CLIA Kit for Mouse RAC-alpha serine/threonine-protein kinase

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U0382b

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96T

CLIA Kit for Mouse RAC-alpha serine/threonine-protein kinase

Cat.:

U0382m

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96T

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CLIA Kit for Mouse RAC-alpha serine/threonine-protein kinase

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U0382h

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96T

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CLIA Kit for Mouse RAC-alpha serine/threonine-protein kinase

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U0382r

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96T

Polyclonal Antibody for Mouse RAC-alpha serine/threonine-protein kinase

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P0382Rb-h

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40ug/0.2ml

Polyclonal Antibody for Mouse RAC-alpha serine/threonine-protein kinase

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P0382Rb-m

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Polyclonal Antibody for Mouse RAC-alpha serine/threonine-protein kinase

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P0382Rb-r

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Polyclonal Antibody for Mouse RAC-alpha serine/threonine-protein kinase

Monoclonal Antibody for Mouse RAC-alpha serine/threonine-protein kinase

Protein for Mouse RAC-alpha serine/threonine-protein kinase

R&D Technical Data

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Precision

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Recovery

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Linearity

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References

1.

"Structure, expression and chromosomal mapping of c-akt: relationship to v-akt and its implications."
Bellacosa A. , Franke T.F. , Gonzalez-Portal M.E. , Datta K. , Taguchi T. , Gardner J. , Cheng J.Q. , Testa J.R. , Tsichlis P.N.
Oncogene8:745-754(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];TISSUE SPECIFICITY
strain: AKR/J.
tissue: Thymus.
2.

"Protein kinase B alpha/Akt1 regulates placental development and fetal growth."
Yang Z.Z. , Tschopp O. , Hemmings-Mieszczak M. , Feng J. , Brodbeck D. , Perentes E. , Hemmings B.A.
J. Biol. Chem.278:32124-32131(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];FUNCTION;PHOSPHORYLATION AT SER-473;DISRUPTION PHENOTYPE;DEVELOPMENTAL STAGE
strain: 129/SvJ.
3.

"Complete nucleotide coding sequence for murine rac (related to A and C kinases) protein kinase."
Bousquets X. , Powell C.T.
Submitted (1992-06) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
4.

"The transcriptional landscape of the mammalian genome."
Carninci P. , Kasukawa T. , Katayama S. , Gough J. , Frith M.C. , Maeda N. , Oyama R. , Ravasi T. , Lenhard B. , Wells C. , Kodzius R. , Shimokawa K. , Bajic V.B. , Brenner S.E. , Batalov S. , Forrest A.R. , Zavolan M. , Davis M.J. , more...
Science309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
strain: NOD.
5.

"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M. , Goodstadt L. , Hillier L.W. , Zody M.C. , Goldstein S. , She X. , Bult C.J. , Agarwala R. , Cherry J.L. , DiCuccio M. , Hlavina W. , Kapustin Y. , Meric P. , Maglott D. , Birtle Z. , Marques A.C. , Graves T. , Zhou S. , more...
PLoS Biol.7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
strain: 129/SvJ.
6.

Mural R.J. , Adams M.D. , Myers E.W. , Smith H.O. , Venter J.C.
Submitted (2005-09) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
7.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
strain: C57BL/6.
tissue: Brain.
8.

"Physiological role of Akt in insulin-stimulated translocation of GLUT4 in transfected rat adipose cells."
Cong L.N. , Chen H. , Li Y. , Zhou L. , McGibbon M.A. , Taylor S.I. , Quon M.J.
Mol. Endocrinol.11:1881-1890(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;MUTAGENESIS OF LYS-179
9.

"Insulin-induced phosphorylation and activation of cyclic nucleotide phosphodiesterase 3B by the serine-threonine kinase Akt."
Kitamura T. , Kitamura Y. , Kuroda S. , Hino Y. , Ando M. , Kotani K. , Konishi H. , Matsuzaki H. , Kikkawa U. , Ogawa W. , Kasuga M.
Mol. Cell. Biol.19:6286-6296(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN PHOSPHORYLATION OF PDE3B
10.

"Flt3 mutations from patients with acute myeloid leukemia induce transformation of 32D cells mediated by the Ras and STAT5 pathways."
Mizuki M. , Fenski R. , Halfter H. , Matsumura I. , Schmidt R. , Muller C. , Gruning W. , Kratz-Albers K. , Serve S. , Steur C. , Buchner T. , Kienast J. , Kanakura Y. , Berdel W.E. , Serve H.
Blood96:3907-3914(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION IN RESPONSE TO FLT3 SIGNALING
11.

"Tcl1 enhances Akt kinase activity and mediates its nuclear translocation."
Pekarsky Y. , Koval A. , Hallas C. , Bichi R. , Tresini M. , Malstrom S. , Russo G. , Tsichlis P. , Croce C.M.
Proc. Natl. Acad. Sci. U.S.A.97:3028-3033(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION
12.

"Reperfusion-activated Akt kinase prevents apoptosis in transgenic mouse hearts overexpressing insulin-like growth factor-1."
Yamashita K. , Kajstura J. , Discher D.J. , Wasserlauf B.J. , Bishopric N.H. , Anversa P. , Webster K.A.
Circ. Res.88:609-614(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
13.

"Phosphorylation of PTP1B at Ser(50) by Akt impairs its ability to dephosphorylate the insulin receptor."
Ravichandran L.V. , Chen H. , Li Y. , Quon M.J.
Mol. Endocrinol.15:1768-1780(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN PHOSPHORYLATION OF PTPN1
14.

"Carboxyl-terminal modulator protein (CTMP), a negative regulator of PKB/Akt and v-Akt at the plasma membrane."
Maira S.-M. , Galetic I. , Brazil D.P. , Kaech S. , Ingley E. , Thelen M. , Hemmings B.A.
Science294:374-380(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH THEM4
15.

"A method to identify serine kinase substrates. Akt phosphorylates a novel adipocyte protein with a Rab GTPase-activating protein (GAP) domain."
Kane S. , Sano H. , Liu S.C.H. , Asara J.M. , Lane W.S. , Garner C.C. , Lienhard G.E.
J. Biol. Chem.277:22115-22118(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN PHOSPHORYLATION OF TBC1D4
16.

"A novel protein kinase B (PKB)/AKT-binding protein enhances PKB kinase activity and regulates DNA synthesis."
Anai M. , Shojima N. , Katagiri H. , Ogihara T. , Sakoda H. , Onishi Y. , Ono H. , Fujishiro M. , Fukushima Y. , Horike N. , Viana A. , Kikuchi M. , Noguchi N. , Takahashi S. , Takata K. , Oka Y. , Uchijima Y. , Kurihara H. , more...
J. Biol. Chem.280:18525-18535(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH CCDC88A;PHOSPHORYLATION AT THR-308 AND SER-473
17.

"Phosphorylation of grb10 regulates its interaction with 14-3-3."
Urschel S. , Bassermann F. , Bai R.Y. , Munch S. , Peschel C. , Duyster J.
J. Biol. Chem.280:16987-16993(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH GRB10
18.

"Large-scale phosphorylation analysis of mouse liver."
Villen J. , Beausoleil S.A. , Gerber S.A. , Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A.104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-129;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Liver.
19.

"A novel Akt/PKB-interacting protein promotes cell adhesion and inhibits familial amyotrophic lateral sclerosis-linked mutant SOD1-induced neuronal death via inhibition of PP2A-mediated dephosphorylation of Akt/PKB."
Nawa M. , Kanekura K. , Hashimoto Y. , Aiso S. , Matsuoka M.
Cell. Signal.20:493-505(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH BTBD10
20.

"O-GlcNAc modulation at Akt1 Ser473 correlates with apoptosis of murine pancreatic beta cells."
Kang E.S. , Han D. , Park J. , Kwak T.K. , Oh M.A. , Lee S.A. , Choi S. , Park Z.Y. , Kim Y. , Lee J.W.
Exp. Cell Res.314:2238-2248(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION AT SER-473
21.

"Phosphoinositide signalling links O-GlcNAc transferase to insulin resistance."
Yang X. , Ongusaha P.P. , Miles P.D. , Havstad J.C. , Zhang F. , So W.V. , Kudlow J.E. , Michell R.H. , Olefsky J.M. , Field S.J. , Evans R.M.
Nature451:964-969(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;GLYCOSYLATION AT SER-473;PHOSPHORYLATION AT THR-308
22.

"DISC1 regulates new neuron development in the adult brain via modulation of AKT-mTOR signaling through KIAA1212."
Kim J.Y. , Duan X. , Liu C.Y. , Jang M.H. , Guo J.U. , Pow-anpongkul N. , Kang E. , Song H. , Ming G.L.
Neuron63:761-773(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
23.

"Cdc2-like kinase 2 is an insulin-regulated suppressor of hepatic gluconeogenesis."
Rodgers J.T. , Haas W. , Gygi S.P. , Puigserver P.
Cell Metab.11:23-34(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH CLK2
24.

"mTORC2 can associate with ribosomes to promote cotranslational phosphorylation and stability of nascent Akt polypeptide."
Oh W.J. , Wu C.C. , Kim S.J. , Facchinetti V. , Julien L.A. , Finlan M. , Roux P.P. , Su B. , Jacinto E.
EMBO J.29:3939-3951(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT THR-450
25.

"Ack1 mediated AKT/PKB tyrosine 176 phosphorylation regulates its activation."
Mahajan K. , Coppola D. , Challa S. , Fang B. , Chen Y.A. , Zhu W. , Lopez A.S. , Koomen J. , Engelman R.W. , Rivera C. , Muraoka-Cook R.S. , Cheng J.Q. , Schoenbrunn E. , Sebti S.M. , Earp H.S. , Mahajan N.P.
PLoS ONE5:E9646-E9646(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION;PHOSPHORYLATION AT TYR-176; THR-308 AND SER-473;MUTAGENESIS OF TYR-176;INTERACTION WITH TNK2
26.

"Protein-tyrosine phosphatase DEP-1 controls receptor tyrosine kinase FLT3 signaling."
Arora D. , Stopp S. , Bohmer S.A. , Schons J. , Godfrey R. , Masson K. , Razumovskaya E. , Ronnstrand L. , Tanzer S. , Bauer R. , Bohmer F.D. , Muller J.P.
J. Biol. Chem.286:10918-10929(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION IN RESPONSE TO FLT3 SIGNALING
27.

"ZNRF1 promotes Wallerian degeneration by degrading AKT to induce GSK3B-dependent CRMP2 phosphorylation."
Wakatsuki S. , Saitoh F. , Araki T.
Nat. Cell Biol.13:1415-1423(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;UBIQUITINATION BY ZNRF1;MUTAGENESIS OF THR-308 AND SER-473
28.

"The protein kinase B/Akt signalling pathway in human malignancy."
Nicholson K.M. , Anderson N.G.
Cell. Signal.14:381-395(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW ON FUNCTION
29.

"Akt signalling in health and disease."
Hers I. , Vincent E.E. , Tavare J.M.
Cell. Signal.23:1515-1527(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW ON FUNCTION
30.

"Akt1 and Akt2: differentiating the aktion."
Heron-Milhavet L. , Khouya N. , Fernandez A. , Lamb N.J.
Histol. Histopathol.26:651-662(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW ON FUNCTION
31.

"KCTD20, a relative of BTBD10, is a positive regulator of Akt."
Nawa M. , Matsuoka M.
BMC Biochem.14:27-27(2013) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH KCTD20

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