Gene name:
PYCARD (ASC;CARD5;TMS1) ;
Protein name:
Apoptosis-associated speck-like protein containing a CARD (hASC) ;
Alternative:
PYD and CARD domain-containing protein ;Caspase recruitment domain-containing protein 5 ;Target of methylation-induced silencing 1 ;
Organism:
Human (Homo sapiens).
General Annotation
Sub Unit:
Forms complexes with other DAPIN domain-containing proteins. Interacts with CIAS1/PYPAF1 and PYDC1.
Function:
Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases.
Subcellular Location:
Cytoplasm
Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures.
Protein Attributes:
50:
MGRARDAILD | ALENLTAEEL | KKFKLKLLSV | PLREGYGRIP | RGALLSMDAL |
100:
DLTDKLVSFY | LETYGAELTA | NVLRDMGLQE | MAGQLQAATH | QGSGAAPAGI |
150:
QAPPQSAAKP | GLHFIDQHRA | ALIARVTNVE | WLLDALYGKV | LTDEQYQAVR |
195:
AEPTNPSKMR | KLFSFTPAWN | WTCKDLLLQA | LRESQSYLVE | DLERS
Vaild Sequence:
Related Databases
Uniprot:
ELISA Kit
CLIA Kit
Polyclonal Antibody
Monoclonal Antibody
Protein
FOR
Mouse
Human
Bovine
Polyclonal Antibody for Human hASC
Polyclonal Antibody for Human hASC
Polyclonal Antibody for Human hASC
Monoclonal Antibody for Human hASC
Monoclonal Antibody for Human hASC
Monoclonal Antibody for Human hASC
R&D Technical Data
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Precision
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Recovery
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Linearity
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References
1.
"ASC, a novel 22-kDa protein, aggregates during apoptosis of human promyelocytic leukemia HL-60 cells."
Masumoto J.
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Taniguchi S.
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Ayukawa K.
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Sarvotham H.
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Kishino T.
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Niikawa N.
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Hidaka E.
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Sagara J.
J. Biol. Chem.274:33835-33838(1999)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
tissue :
Leukemia .
2.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2)
tissue :
Fibroblast .
3.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE (ISOFORM 2);FUNCTION (ISOFORM 2);MASS SPECTROMETRY (ISOFORM 2)
4.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE (ISOFORM 1)
5.
"CARD5 protein is a CARD/PYRIN family member that is involved in apoptosis signal transduction."
Bertin J.
Submitted (2001-05) to the EMBL/GenBank/DDBJ databases
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE (ISOFORM 1)
6.
"Complete sequencing and characterization of 21,243 full-length human cDNAs."
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Yamazaki M.
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Komiyama M.
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Tashiro H.
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Tanigami A.
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Ono T.
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Yamada K.
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Fujii Y.
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Ozaki K.
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Ohara O.
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Isogai T.
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Sugano S.
more...
Nat. Genet.36:40-45(2004)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
tissue :
Colon mucosa .
7.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3);NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4-195 (ISOFORM 1)
tissue :
Lymph .
tissue :
Pancreas .
8.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : FUNCTION;SUBCELLULAR LOCATION
9.
"Human CARD12 is a novel CED4/Apaf-1 family member that induces apoptosis."
Geddes B.J.
,
Wang L.
,
Huang W.-J.
,
Lavellee M.
,
Manji G.A.
,
Brown M.
,
Jurman M.
,
Cao J.
,
Morgenstern J.
,
Merriam S.
,
Glucksmann M.A.
,
DiStefano P.S.
,
Bertin J.
Biochem. Biophys. Res. Commun.284:77-82(2001)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : INTERACTION WITH NLRC4
10.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : INTERACTION WITH MEFV
11.
"PYPAF1: a PYRIN-containing APAF1-like protein that assembles with ASC and activates NF-kB."
Manji G.A.
,
Wang L.
,
Geddes B.J.
,
Brown M.
,
Merriam S.
,
Al-Garawi A.
,
Mak S.
,
Lora J.M.
,
Briskin M.
,
Jurman M.
,
Cao J.
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DiStefano P.S.
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Bertin J.
J. Biol. Chem.277:11570-11575(2002)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : INTERACTION WITH NLRP3;DOMAIN
12.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : INTERACTION WITH CASP1; NLRC4 AND CARD16
13.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : FUNCTION;INTERACTION WITH CHUK AND IKBKB
14.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : IDENTIFICATION IN NLPR1 INFLAMMASOME
15.
"ASC is an activating adaptor for NF-kappa B and caspase-8-dependent apoptosis."
Masumoto J.
,
Dowds T.A.
,
Schaner P.
,
Chen F.F.
,
Ogura Y.
,
Li M.
,
Zhu L.
,
Katsuyama T.
,
Sagara J.
,
Taniguchi S.
,
Gumucio D.L.
,
Nunez G.
,
Inohara N.
Biochem. Biophys. Res. Commun.303:69-73(2003)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : FUNCTION IN APOPTOSIS;INTERACTION WITH CASP8;MUTAGENESIS OF LEU-12
16.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : INTERACTION WITH PYDC1;PHOSPHORYLATION
17.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : INTERACTION WITH CASP1 AND RIPK2
18.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : FUNCTION IN NLRP2 AND NLRP3 INFLAMMASOMES;INTERACTION WITH NLRP2 AND NLRP3;SUBCELLULAR LOCATION
19.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : INTERACTION WITH NLRP10
20.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : INTERACTION WITH NLRP3
21.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : INTERACTION WITH NLRP2;MUTAGENESIS OF GLU-13
22.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : FUNCTION IN APOPTOSIS;SUBCELLULAR LOCATION;INTERACTION WITH BAX
23.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : SELF-ASSOCIATION;MUTAGENESIS OF ILE-8; LEU-12; GLU-13; LEU-15; GLU-19; LEU-20; LYS-21; PHE-23; LEU-25; LYS-26; LEU-27; PRO-40; ARG-41; LEU-45; MET-47; ASP-48; LEU-52; LEU-56; GLU-62; GLU-67; LEU-68; VAL-72 AND MET-76
24.
"Cryopyrin and pyrin activate caspase-1, but not NF-kappaB, via ASC oligomerization."
Yu J.W.
,
Wu J.
,
Zhang Z.
,
Datta P.
,
Ibrahimi I.
,
Taniguchi S.
,
Sagara J.
,
Fernandes-Alnemri T.
,
Alnemri E.S.
Cell Death Differ.13:236-249(2006)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : ASSOCIATION WITH INFLAMMASOMES
25.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : FUNCTION;INTERACTION WITH CASP1
26.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : FUNCTION
27.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : FUNCTION;SUBUNIT
28.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : FUNCTION IN NLRP1 INFLAMMASOME
29.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : FUNCTION IN APOPTOSIS
30.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : INTERACTION WITH PYDC2
31.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : INTERACTION WITH PYDC2
32.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : INTERACTION WITH PYDC1;MUTAGENESIS OF GLU-13; TYR-36 AND ASP-48
33.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : FUNCTION;SUBCELLULAR LOCATION
34.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : FUNCTION
35.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : FUNCTION IN AIM2 INFLAMMASOME;INTERACTION WITH AIM2
36.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : FUNCTION IN AIM2 INFLAMMASOME;INTERACTION WITH AIM2
37.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : FUNCTION (ISOFORMS 2 AND 3)
38.
"Recognition of RNA virus by RIG-I results in activation of CARD9 and inflammasome signaling for interleukin 1 beta production."
Poeck H.
,
Bscheider M.
,
Gross O.
,
Finger K.
,
Roth S.
,
Rebsamen M.
,
Hannesschlager N.
,
Schlee M.
,
Rothenfusser S.
,
Barchet W.
,
Kato H.
,
Akira S.
,
Inoue S.
,
Endres S.
,
Peschel C.
,
Hartmann G.
,
Hornung V.
,
Ruland J.
Nat. Immunol.11:63-69(2010)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : INTERACTION WITH DDX58
39.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
40.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : INTERACTION WITH IFI16
41.
"The NLR adaptor ASC/PYCARD regulates DUSP10, mitogen-activated protein kinase (MAPK), and chemokine induction independent of the inflammasome."
Taxman D.J.
,
Holley-Guthrie E.A.
,
Huang M.T.
,
Moore C.B.
,
Bergstralh D.T.
,
Allen I.C.
,
Lei Y.
,
Gris D.
,
Ting J.P.
J. Biol. Chem.286:19605-19616(2011)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : FUNCTION
42.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : FUNCTION
43.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : SUBCELLULAR LOCATION
44.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : STRUCTURE BY NMR OF 1-91;FUNCTION OF DAPIN DOMAIN
45.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : STRUCTURE BY NMR