Brain-derived neurotrophic factor (Protein name
), BDNF_RAT from NCBI database.
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General Annotation
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Antigen Annotation
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3D
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Predicted Eptitope
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Vaild Sequence
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Gene name:
Bdnf;
Protein name:
Brain-derived neurotrophic factor(BDNF);
Alternative:
Organism:
Rat (Rattus norvegicus).
General Annotation
Sub Unit:
Monomers and homodimers. Binds to NTRK2/TRKB.
Function:
During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS.
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s
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.1%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.4%
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.4%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.1%
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Recovery
Recovery was determined by spiking various levels of BDNF into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
93
91-98
Plasma
94
93-100
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Recovery was determined by spiking various levels of BDNF into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
99
94-103
Plasma
98
96-100
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Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BDNF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1：2
1:4
1:8
1:16
serum(n=5)
92-96%
90-100%
95-99%
92-96%
EDTA plasma(n=5)
96-103%
93-96%
96-98%
93-102%
heparin plasma(n=5)
89-104%
91-96%
89-92%
87-97%
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BDNF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1：2
1:4
1:8
1:16
serum(n=5)
96-101%
93-99%
95-105%
92-96%
EDTA plasma(n=5)
99-104%
94-106%
99-110%
96-99%
heparin plasma(n=5)
95-102%
97-102%
101-109%
91-96%
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"Reconstituting BDNF gene to bone marrow mesenchymal stem cells: serving as portals in the therapy of Parkinson's disease." Zhao L.X.
,
Zhang J.
,
Wang D.M.
,
Yu X.J.
,
Yue W.
,
Pei X.T.
,
Xu X.H.
Submitted (2002-11) to the EMBL/GenBank/DDBJ databases
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Cited for: NUCLEOTIDE SEQUENCE [MRNA]
Vaild Sequence:
