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Index > Protein center > ALCAM(Gene name) > Human
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  • ALCAM (Gene name),
  • CD166 antigen (Protein name ),  CD166_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Related Databases
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  • Gene name:
    ALCAM(MEMD);
    Protein name:
    CD166 antigen;
    Alternative:
    Activated leukocyte cell adhesion molecule;
    CD:
    CD166;
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    N/A
    Function:
    Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system.
    Subcellular Location:
    Membrane Single-pass type I membrane protein
    Protein Attributes:
    Sequence length:
    583
    Sequence:
    50:
    MESKGASSCR | LLFCLLISAT | VFRPGLGWYT | VNSAYGDTII | IPCRLDVPQN | 
    100:
    LMFGKWKYEK | PDGSPVFIAF | RSSTKKSVQY | DDVPEYKDRL | NLSENYTLSI | 
    150:
    SNARISDEKR | FVCMLVTEDN | VFEAPTIVKV | FKQPSKPEIV | SKALFLETEQ | 
    200:
    LKKLGDCISE | DSYPDGNITW | YRNGKVLHPL | EGAVVIIFKK | EMDPVTQLYT | 
    250:
    MTSTLEYKTT | KADIQMPFTC | SVTYYGPSGQ | KTIHSEQAVF | DIYYPTEQVT | 
    300:
    IQVLPPKNAI | KEGDNITLKC | LGNGNPPPEE | FLFYLPGQPE | GIRSSNTYTL | 
    350:
    TDVRRNATGD | YKCSLIDKKS | MIASTAITVH | YLDLSLNPSG | EVTRQIGDAL | 
    400:
    PVSCTISASR | NATVVWMKDN | IRLRSSPSFS | SLHYQDAGNY | VCETALQEVE | 
    450:
    GLKKRESLTL | IVEGKPQIKM | TKKTDPSGLS | KTIICHVEGF | PKPAIQWTIT | 
    500:
    GSGSVINQTE | ESPYINGRYY | SKIIISPEEN | VTLTCTAENQ | LERTVNSLNV | 
    550:
    SAISIPEHDE | ADEISDENRE | KVNDQAKLIV | GIVVGLLLAA | LVAGVVYWLY | 
    583:
    MKKSKTASKH | VNKDLGNMEE | NKKLEENNHK | TEA
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
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    Related Databases
    KEGG:
    Pfam:
    String:
    UniGene:
    MIM:
    SMR:
    Uniprot:
     
    FOR
    ELISA Kit for Human CD166 antigen
    Cat.:
    E0002h
    Price:
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    MSDS:
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    Packing:
    96T
    Range:
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    ELISA Kit for Human CD166 antigen
    Cat.:
    E0002m
    Price:
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    MSDS:
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    Packing:
    96T
    Range:
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    ELISA Kit for Human CD166 antigen
    Cat.:
    E0002r
    Price:
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    MSDS:
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    Packing:
    96T
    Range:
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    ELISA Kit for Human CD166 antigen
    Cat.:
    E0002b
    Price:
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    MSDS:
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    Packing:
    96T
    ELISA Kit for Human CD166 antigen
    MSDS:
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    ELISA Kit for Human CD166 antigen
    Cat.:
    E0002c
    Price:
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    MSDS:
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    Packing:
    96T
    ELISA Kit for Human CD166 antigen
    Cat.:
    E0002d
    Price:
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Human CD166 antigen
    Cat.:
    U0002c
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human CD166 antigen
    Cat.:
    U0002d
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human CD166 antigen
    Cat.:
    U0002r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human CD166 antigen
    MSDS:
    Please sign in first.
    CLIA Kit for Human CD166 antigen
    Cat.:
    U0002m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human CD166 antigen
    Cat.:
    U0002h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human CD166 antigen
    Cat.:
    U0002b
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Human CD166 antigen
    Cat.:
    P0002Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human CD166 antigen
    Polyclonal Antibody for Human CD166 antigen
    Cat.:
    P0002Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human CD166 antigen
    Polyclonal Antibody for Human CD166 antigen
    Polyclonal Antibody for Human CD166 antigen
    Cat.:
    P0002Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human CD166 antigen
    Monoclonal Antibody for Human CD166 antigen
    Monoclonal Antibody for Human CD166 antigen
    Monoclonal Antibody for Human CD166 antigen
    Monoclonal Antibody for Human CD166 antigen
    Monoclonal Antibody for Human CD166 antigen
    Monoclonal Antibody for Human CD166 antigen
    Monoclonal Antibody for Human CD166 antigen
    Protein for Human CD166 antigen
    Protein for Human CD166 antigen
    Protein for Human CD166 antigen
    Protein for Human CD166 antigen
    Protein for Human CD166 antigen
    Protein for Human CD166 antigen
    Protein for Human CD166 antigen

    R&D Technical Data
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    s
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    测试

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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤4.3%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤6.9%

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    Recovery
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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    93-105

    Plasma

    101

    95-106

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    95-104%

    97-106%

    95-103%

    94-107%

    EDTA plasma(n=5)

    91-97%

    90-100%

    93-99%

    92-103%

    heparin plasma(n=5)

    94-103%

     

    96-108%

    97-109%

    93-106%

     

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    References
    1. 1.
      "Cloning, mapping, and characterization of activated leukocyte-cell adhesion molecule (ALCAM), a CD6 ligand."
      Bowen M.A. , Patel D.D. , Li X. , Modrell B. , Malacko A.R. , Wang W.-C. , Marquardt H. , Neubauer M. , Pesando J.M. , Francke U. , Haynes B.F. , Aruffo A.
      J. Exp. Med.181:2213-2220(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);VARIANT MET-301;PARTIAL PROTEIN SEQUENCE
    2. 2.
      "Molecular isolation and characterization of a soluble isoform of activated leukocyte cell adhesion molecule that modulates endothelial cell function."
      Ikeda K. , Quertermous T.
      J. Biol. Chem.279:55315-55323(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3);SUBCELLULAR LOCATION (ISOFORM 3)
    3. 3.
      "A novel alternatively spliced variant of activated leukocyte cell adhesion molecule (ALCAM/CD166) resulting from a deletion in the extracellular membrane proximal stem."
      Abe Y. , Bui-Thanh N.-A. , Smith C.W. , Ballantyne C.M. , Burns A.R.
      Submitted (2006-04) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2)
    4. 4.
      "The DNA sequence, annotation and analysis of human chromosome 3."
      Muzny D.M. , Scherer S.E. , Kaul R. , Wang J. , Yu J. , Sudbrak R. , Buhay C.J. , Chen R. , Cree A. , Ding Y. , Dugan-Rocha S. , Gill R. , Gunaratne P. , Harris R.A. , Hawes A.C. , Hernandez J. , Hodgson A.V. , Hume J. , more...
      Nature440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    5. 5.
      "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
      Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4)
      tissue: Lung.
      tissue: Placenta.
    6. 6.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3)
      tissue: Brain.
      tissue: Lung.
    7. 7.
      "MEMD, a new cell adhesion molecule in metastasizing human melanoma cell lines, is identical to ALCAM (activated leukocyte cell adhesion molecule)."
      Degen W.G. , van Kempen L.C. , Gijzen E.G. , van Groningen J.J. , van Kooyk Y. , Bloemers H.P.J. , Swart G.W.
      Am. J. Pathol.152:805-813(1998) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-583 (ISOFORM 1);TISSUE SPECIFICITY
    8. 8.
      "Recognition of diverse proteins by members of the immunoglobulin superfamily: delineation of the receptor binding site in the human CD6 ligand ALCAM."
      Skonier J.E. , Bowen M.A. , Emswiler J. , Aruffo A. , Bajorath J.
      Biochemistry35:12287-12291(1996) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DOMAIN CD6 BINDING
    9. 9.
      "Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry."
      Zhang H. , Li X.-J. , Martin D.B. , Aebersold R.
      Nat. Biotechnol.21:660-666(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION AT ASN-91; ASN-167; ASN-480 AND ASN-499
    10. 10.
      "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
      Liu T. , Qian W.-J. , Gritsenko M.A. , Camp D.G. II , Monroe M.E. , Moore R.J. , Smith R.D.
      J. Proteome Res.4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-95; ASN-265 AND ASN-499
      tissue: Plasma.
    11. 11.
      "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
      Chen R. , Jiang X. , Sun D. , Han G. , Wang F. , Ye M. , Wang L. , Zou H.
      J. Proteome Res.8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-91; ASN-95; ASN-457 AND ASN-480
      tissue: Liver.
    12. 12.
      "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
      Wollscheid B. , Bausch-Fluck D. , Henderson C. , O'Brien R. , Bibel M. , Schiess R. , Aebersold R. , Watts J.D.
      Nat. Biotechnol.27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-457 AND ASN-466
      tissue: Leukemic T-cell.
    13. 13.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
    14. 14.
      "Molecular model of the N-terminal receptor-binding domain of the human CD6 ligand ALCAM."
      Bajorath J. , Bowen M.A. , Aruffo A.
      Protein Sci.4:1644-1647(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: 3D-STRUCTURE MODELING OF 28-133
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