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Index > Protein center > Alcam(Gene name) > Rat
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  • Alcam (Gene name),
  • CD166 antigen (Protein name ),  CD166_RAT from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Predicted Eptitope
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  • Gene name:
    Alcam;
    Protein name:
    CD166 antigen;
    Alternative:
    SB-10 antigen;Protein MEMD;KG-CAM;Activated leukocyte cell adhesion molecule;HB2;
    CD:
    CD166;
    Organism:
    Rat (Rattus norvegicus). 
    General Annotation
    Sub Unit:
    N/A
    Function:
    Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system. May be involved in osteogenesis. May play an important role in the development of a variety of mesenchymal tissues.
    Subcellular Location:
    Membrane Single-pass type I membrane protein
    Protein Attributes:
    Sequence length:
    583
    Sequence:
    50:
    MASKGSPSCR | LVFCLLISAA | VLRPGLGWYT | VNSAYGDTIV | MPCRLDVPQN | 
    100:
    LMFGKWKYEK | PDGSPVFIAF | RSSTKKSVQY | DDVPEYKDRL | SLSENYTLSI | 
    150:
    NNAKISDEKR | FVCMLVTEDN | VFEAPTLVKV | FKQPSKPEIV | NRAAFLETEQ | 
    200:
    LKKLGDCISR | DSYPDGNITW | YRNGKVLQPV | DGEVSILFKK | EIDPGTQLYT | 
    250:
    MTSSLEYKTT | KSDIQMPFTC | SVTYYGPSGQ | KTIYSEQAIF | DIYYPTEQVT | 
    300:
    IQVLPPKNAI | KEGDNITLQC | LGNGNPPPEE | FMFYLPGQAE | GIRSSNTYTL | 
    350:
    TDVRRNATGD | YKCSLIDQRN | MAASTTITVH | YLDLSLNPSG | EVTKQIGDTL | 
    400:
    PVSCTISASR | NATVVWMKDN | IRLRSSPSFS | SLHYQDAGNY | VCETALQEVE | 
    450:
    GLKKRESLTL | IVEGKPQIKM | TKKTDPSGLS | KTIICHVEGF | PKPAIQWTIT | 
    500:
    GSGSVINQTE | ESPYINGRYY | SKIIISPEEN | VTLTCTAENQ | LERTVNSLNV | 
    550:
    SAISIPEHDE | ADDISDENRE | KVNDQAKLIV | GIVVGLLLAA | LVAGVVYWLY | 
    583:
    MKKSKTASKH | VNKDLGNMEE | NKKLEENNHK | TEA
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
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    Related Databases
    UniGene:
    Pfam:
    KEGG:
    String:
    Uniprot:
     
    FOR
    ELISA Kit for Rat CD166 antigen
    Cat.:
    E0002h
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    96T
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    ELISA Kit for Rat CD166 antigen
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    E0002m
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    ELISA Kit for Rat CD166 antigen
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    E0002r
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    ELISA Kit for Rat CD166 antigen
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    E0002b
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    ELISA Kit for Rat CD166 antigen
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    ELISA Kit for Rat CD166 antigen
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    E0002c
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    ELISA Kit for Rat CD166 antigen
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    E0002d
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    Packing:
    96T
    CLIA Kit for Rat CD166 antigen
    Cat.:
    U0002c
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    96T
    CLIA Kit for Rat CD166 antigen
    Cat.:
    U0002d
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    Packing:
    96T
    CLIA Kit for Rat CD166 antigen
    Cat.:
    U0002r
    Price:
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    MSDS:
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    Packing:
    96T
    Range:
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    CLIA Kit for Rat CD166 antigen
    MSDS:
    Please sign in first.
    CLIA Kit for Rat CD166 antigen
    Cat.:
    U0002m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
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    CLIA Kit for Rat CD166 antigen
    Cat.:
    U0002h
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    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
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    CLIA Kit for Rat CD166 antigen
    Cat.:
    U0002b
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    MSDS:
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    Packing:
    96T
    Polyclonal Antibody for Rat CD166 antigen
    Cat.:
    P0002Rb-h
    Price:
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat CD166 antigen
    Polyclonal Antibody for Rat CD166 antigen
    Cat.:
    P0002Rb-m
    Price:
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat CD166 antigen
    Polyclonal Antibody for Rat CD166 antigen
    Polyclonal Antibody for Rat CD166 antigen
    Cat.:
    P0002Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat CD166 antigen
    Monoclonal Antibody for Rat CD166 antigen
    Monoclonal Antibody for Rat CD166 antigen
    Monoclonal Antibody for Rat CD166 antigen
    Monoclonal Antibody for Rat CD166 antigen
    Monoclonal Antibody for Rat CD166 antigen
    Monoclonal Antibody for Rat CD166 antigen
    Monoclonal Antibody for Rat CD166 antigen
    Protein for Rat CD166 antigen
    Protein for Rat CD166 antigen
    Protein for Rat CD166 antigen
    Protein for Rat CD166 antigen
    Protein for Rat CD166 antigen
    Protein for Rat CD166 antigen
    Protein for Rat CD166 antigen

    R&D Technical Data
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    s
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    测试

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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤4.3%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤6.9%

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    Recovery
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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    93-105

    Plasma

    101

    95-106

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    95-104%

    97-106%

    95-103%

    94-107%

    EDTA plasma(n=5)

    91-97%

    90-100%

    93-99%

    92-103%

    heparin plasma(n=5)

    94-103%

     

    96-108%

    97-109%

    93-106%

     

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    References
    1. 1.
      "Cloning and characterization of HB2, a candidate high density lipoprotein receptor. Sequence homology with members of the immunoglobulin superfamily of membrane proteins."
      Matsumoto A. , Mitchell A. , Kurata H. , Pyle L. , Kondo K. , Itakura H. , Fidge N.
      J. Biol. Chem.272:16778-16782(1997) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PARTIAL PROTEIN SEQUENCE
    2. 2.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Prostate.
    3. 3.
      "Distribution and characteristics of a 90 kDa protein, KG-CAM, in the rat CNS."
      Peduzzi J.D. , Irwin M.H. , Geisert E.E. Jr.
      Brain Res.640:296-307(1994) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 29-38
    4. 4.
      Lubec G. , Kang S.U. , Lubec S.
      Submitted (2007-09) to the UniProtKB
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 45-55; 58-71; 77-87; 111-129; 143-152; 154-160; 176-189; 306-312 AND 406-423;IDENTIFICATION BY MASS SPECTROMETRY
      strain: Sprague-Dawley.
      tissue: Brain.
    5. 5.
      "Mesenchymal stem cell surface antigen SB-10 corresponds to activated leukocyte cell adhesion molecule and is involved in osteogenic differentiation."
      Bruder S.P. , Ricalton N.S. , Boynton R.E. , Connolly T.J. , Jaiswal N. , Zaia J. , Barry F.P.
      J. Bone Miner. Res.13:655-663(1998) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 94-156 AND 270-361
      tissue: Mesenchymal cell.
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