CDKN1A ELISA Kit for Human(Homo sapiens) | EIAab.comCDKN1A ELISA Kit for Human(Homo sapiens)

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Index > Protein center > CDKN1A(Gene name) > Human

CDKN1A (Gene name),

Cyclin-dependent kinase inhibitor 1 (Protein name ), CDN1A_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

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Gene name:

CDKN1A(CAP20;CDKN1;CIP1;MDA6;PIC1;SDI1;WAF1);

Protein name:

Cyclin-dependent kinase inhibitor 1;

Alternative:

Melanoma differentiation-associated protein 6(MDA-6);CDK-interacting protein 1;p21;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Interacts with HDAC1; the interaction is prevented by competitive binding of C10orf90/FATS to HDAC1 facilitating acetylation and protein stabilization of CDKN1A/p21 (By similarity). Interacts with MKRN1. Interacts with PSMA3. Interacts with PCNA. Component of the ternary complex, cyclin D-CDK4-CDKN1A. Interacts (via its N-terminal domain) with CDK4; the interaction promotes the assembly of the cyclin D-CDK4 complex, its nuclear translocation and promotes the cyclin D-dependent enzyme activity of CDK4. Binding to CDK2 leads to CDK2/cyclin E inactivation at the G1-S phase DNA damage checkpoint, thereby arresting cells at the G1-S transition during DNA repair.

Function:

May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.

Subcellular Location:

Cytoplasm Nucleus

Protein Attributes:

Sequence length:

164

Sequence:

50:

100:

150:

164:

YHSKRRLIFS | KRKP

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

UniGene:

Hs.370771

String:

P38936

Pfam:

PF02234

KEGG:

hsa:1026

MIM:

116899

STRING:

9606.ENSP00000244741

SMR:

P38936

Uniprot:

P38936

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ELISA Kit for Human Cyclin-dependent kinase inhibitor 1

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E9387m

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96T

ELISA Kit for Human Cyclin-dependent kinase inhibitor 1

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E9387h

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96T

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CLIA Kit for Human Cyclin-dependent kinase inhibitor 1

Cat.:

U9387m

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96T

CLIA Kit for Human Cyclin-dependent kinase inhibitor 1

Cat.:

U9387h

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96T

Polyclonal Antibody for Human Cyclin-dependent kinase inhibitor 1

Cat.:

P9387Rb-h

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40ug/0.2ml

Polyclonal Antibody for Human Cyclin-dependent kinase inhibitor 1

Cat.:

P9387Rb-m

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Packing:

40ug/0.2ml

Monoclonal Antibody for Human Cyclin-dependent kinase inhibitor 1

Protein for Human Cyclin-dependent kinase inhibitor 1

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

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Recovery

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Linearity

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References

1.

"The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases."
Harper J.W. , Adami G.R. , Wei N. , Keyomarsi K. , Elledge S.J.
Cell75:805-816(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];FUNCTION;INDUCTION
2.

"WAF1, a potential mediator of p53 tumor suppression."
El-Deiry W.S. , Tokino T. , Velculescu V.E. , Levy D.B. , Parsons R. , Trent J.M. , Lin D. , Mercer W.E. , Kinzler K.W. , Vogelstein B.
Cell75:817-825(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];INDUCTION
3.

"p21 is a universal inhibitor of cyclin kinases."
Xiong Y. , Hannon G.J. , Zhang H. , Casso D. , Kobayashi R. , Beach D.
Nature366:701-704(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
4.

"Use of a sensitive and efficient subtraction hybridization protocol for the identification of genes differentially regulated during the induction of differentiation in human melanoma cells."
Jiang H. , Fisher P.B.
Mol. Cell. Differ.1:285-299(1993)

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
5.

"The melanoma differentiation-associated gene mda-6, which encodes the cyclin-dependent kinase inhibitor p21, is differentially expressed during growth, differentiation and progression in human melanoma cells."
Jiang H. , Lin J. , Su Z.Z. , Herlyn M. , Kerbel R.S. , Weissman B.E. , Welch D.R. , Fisher P.B.
Oncogene10:1855-1864(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
6.

"Cloning of senescent cell-derived inhibitors of DNA synthesis using an expression screen."
Noda A. , Ning Y. , Venable S.F. , Pereira-Smith O.M. , Smith J.R.
Exp. Cell Res.211:90-98(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
7.

"Two variants of the CIP1/WAF1 gene occur together and are associated with human cancer."
Mousses S. , Oezcelik H. , Lee P.D. , Malkin D. , Bull S.B. , Andrulis I.L.
Hum. Mol. Genet.4:1089-1092(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];VARIANT ARG-31
8.

NIEHS SNPs program
Submitted (2002-04) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANT ARG-31
9.

"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N. , Chen X. , Rolfs A. , Halleck A. , Hines L. , Eisenstein S. , Koundinya M. , Raphael J. , Moreira D. , Kelley T. , LaBaer J. , Lin Y. , Phelan M. , Farmer A.
Submitted (2003-05) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
10.

"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L. , Schick M. , Neubert P. , Schatten R. , Henze S. , Korn B.
Submitted (2004-06) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
11.

"Human protein factory for converting the transcriptome into an in vitro-expressed proteome."
Goshima N. , Kawamura Y. , Fukumoto A. , Miura A. , Honma R. , Satoh R. , Wakamatsu A. , Yamamoto J. , Kimura K. , Nishikawa T. , Andoh T. , Iida Y. , Ishikawa K. , Ito E. , Kagawa N. , Kaminaga C. , Kanehori K. , Kawakami B. , more...
Nat. Methods5:1011-1017(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
12.

"The DNA sequence and analysis of human chromosome 6."
Mungall A.J. , Palmer S.A. , Sims S.K. , Edwards C.A. , Ashurst J.L. , Wilming L. , Jones M.C. , Horton R. , Hunt S.E. , Scott C.E. , Gilbert J.G.R. , Clamp M.E. , Bethel G. , Milne S. , Ainscough R. , Almeida J.P. , Ambrose K.D. , Andrews T.D. , more...
Nature425:805-811(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
13.

Mural R.J. , Istrail S. , Sutton G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
14.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA];VARIANT ARG-31
tissue: Eye.
tissue: Lung.
15.

"N-acetylation and ubiquitin-independent proteasomal degradation of p21(Cip1)."
Chen X. , Chi Y. , Bloecher A. , Aebersold R. , Clurman B.E. , Roberts J.M.
Mol. Cell16:839-847(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 2-16;ACETYLATION AT SER-2;IDENTIFICATION BY MASS SPECTROMETRY
16.

"Reversible phosphorylation at the C-terminal regulatory domain of p21(Waf1/Cip1) modulates proliferating cell nuclear antigen binding."
Scott M.T. , Morrice N. , Ball K.L.
J. Biol. Chem.275:11529-11537(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 136-148;PHOSPHORYLATION AT THR-145; SER-146 AND SER-160;IDENTIFICATION BY MASS SPECTROMETRY
17.

"New functional activities for the p21 family of CDK inhibitors."
LaBaer J. , Garrett M.D. , Stevenson L.F. , Slingerland J.M. , Sandhu C. , Chou H.S. , Fattaey A. , Harlow E.
Genes Dev.11:847-862(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION;INTERACTION WITH CDK4 AND CCND1 IN THE CYCLIN D-CDK4-CDKN1A COMPLEX
18.

"A degradation signal located in the C-terminus of p21WAF1/CIP1 is a binding site for the C8 alpha-subunit of the 20S proteasome."
Touitou R. , Richardson J. , Bose S. , Nakanishi M. , Rivett J. , Allday M.J.
EMBO J.20:2367-2375(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH PSMA3
19.

"Akt-dependent phosphorylation of p21(Cip1) regulates PCNA binding and proliferation of endothelial cells."
Roessig L. , Jadidi A.S. , Urbich C. , Badorff C. , Zeiher A.M. , Dimmeler S.
Mol. Cell. Biol.21:5644-5657(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT THR-145;MUTAGENESIS OF THR-145 AND SER-146;SUBCELLULAR LOCATION
20.

"Phosphorylation of the cell cycle inhibitor p21Cip1/WAF1 by Pim-1 kinase."
Wang Z. , Bhattacharya N. , Mixter P.F. , Wei W. , Sedivy J. , Magnuson N.S.
Biochim. Biophys. Acta1593:45-55(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT THR-145 BY PIM1;SUBCELLULAR LOCATION;INTERACTION WITH PIM1
21.

"N-Terminal ubiquitination of extracellular signal-regulated kinase 3 and p21 directs their degradation by the proteasome."
Coulombe P. , Rodier G. , Bonneil E. , Thibault P. , Meloche S.
Mol. Cell. Biol.24:6140-6150(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: UBIQUITINATION AT SER-2
22.

"Only Akt1 is required for proliferation, while Akt2 promotes cell cycle exit through p21 binding."
Heron-Milhavet L. , Franckhauser C. , Rana V. , Berthenet C. , Fisher D. , Hemmings B.A. , Fernandez A. , Lamb N.J.
Mol. Cell. Biol.26:8267-8280(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT THR-145
23.

"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Beausoleil S.A. , Villen J. , Gerber S.A. , Rush J. , Gygi S.P.
Nat. Biotechnol.24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-130;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
24.

"PCNA-dependent regulation of p21 ubiquitylation and degradation via the CRL4Cdt2 ubiquitin ligase complex."
Abbas T. , Sivaprasad U. , Terai K. , Amador V. , Pagano M. , Dutta A.
Genes Dev.22:2496-2506(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: UBIQUITINATION;DOMAIN PIP-BOX K+4 MOTIF;INTERACTION WITH PCNA;MUTAGENESIS OF SER-114 AND 144-GLN--PHE-150;PHOSPHORYLATION AT SER-114
25.

"The CRL4Cdt2 ubiquitin ligase targets the degradation of p21Cip1 to control replication licensing."
Kim Y. , Starostina N.G. , Kipreos E.T.
Genes Dev.22:2507-2519(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: UBIQUITINATION
26.

"CDK inhibitor p21 is degraded by a proliferating cell nuclear antigen-coupled Cul4-DDB1Cdt2 pathway during S phase and after UV irradiation."
Nishitani H. , Shiomi Y. , Iida H. , Michishita M. , Takami T. , Tsurimoto T.
J. Biol. Chem.283:29045-29052(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: UBIQUITINATION;DOMAIN PIP-BOX K+4 MOTIF;MUTAGENESIS OF 147-MET--TYR-151 AND 154-LYS--ARG-156;INTERACTION WITH PCNA
27.

"A quantitative atlas of mitotic phosphorylation."
Dephoure N. , Zhou C. , Villen J. , Beausoleil S.A. , Bakalarski C.E. , Elledge S.J. , Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A.105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-130;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
28.

"A dual role of Cdk2 in DNA damage response."
Satyanarayana A. , Kaldis P.
Cell Div.4:9-9(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW ON DNA REPAIR;INTERACTION WITH CDK2
29.

"Differential regulation of p53 and p21 by MKRN1 E3 ligase controls cell cycle arrest and apoptosis."
Lee E.-W. , Lee M.-S. , Camus S. , Ghim J. , Yang M.-R. , Oh W. , Ha N.-C. , Lane D.P. , Song J.
EMBO J.28:2100-2113(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: UBIQUITINATION;INTERACTION WITH MKRN1
30.

"Ionizing radiation induces ATM-independent degradation of p21Cip1 in transformed cells."
Stuart S.A. , Wang J.Y.
J. Biol. Chem.284:15061-15070(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: UBIQUITINATION
31.

"Pim-2 phosphorylation of p21(Cip1/WAF1) enhances its stability and inhibits cell proliferation in HCT116 cells."
Wang Z. , Zhang Y. , Gu J.J. , Davitt C. , Reeves R. , Magnuson N.S.
Int. J. Biochem. Cell Biol.42:1030-1038(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT THR-145 BY PIM2
32.

"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P. , Lasa M. , Polevoda B. , Gazquez C. , Elosegui-Artola A. , Kim D.S. , De Juan-Pardo E. , Demeyer K. , Hole K. , Larrea E. , Timmerman E. , Prieto J. , Arnesen T. , Sherman F. , Gevaert K. , Aldabe R.
Proc. Natl. Acad. Sci. U.S.A.109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
33.

"ZNF313 is a novel cell cycle activator with an E3 ligase activity inhibiting cellular senescence by destabilizing p21(WAF1.)."
Han J. , Kim Y.L. , Lee K.W. , Her N.G. , Ha T.K. , Yoon S. , Jeong S.I. , Lee J.H. , Kang M.J. , Lee M.G. , Ryu B.K. , Baik J.H. , Chi S.G.
Cell Death Differ.20:1055-1067(2013) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: UBIQUITINATION BY RNF114
34.

"Structure of the C-terminal region of p21(WAF1/CIP1) complexed with human PCNA."
Gulbis J.M. , Kelman Z. , Hurwitz J. , O'Donnell M. , Kuriyan J.
Cell87:297-306(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 139-160 IN COMPLEX WITH PCNA

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