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Index > Protein center > ACE(Gene name) > Chicken
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  • ACE (Gene name),
  • Angiotensin-converting enzyme (Protein name ),  ACE_CHICK from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    ACE(DCP1);
    Protein name:
    Angiotensin-converting enzyme(ACE);
    Alternative:
    Dipeptidyl carboxypeptidase I;Kininase II;
    Organism:
    Chicken (Gallus gallus). 
    General Annotation
    Sub Unit:
    N/A
    Function:
    Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin.
    Subcellular Location:
    Membrane Single-pass type I membrane protein
    Protein Attributes:
    Sequence length:
    1193
    Sequence:
    50:
    AKELYGNIWS | NFSDPQLKKI | IGSIQTLGPS | NLPLDKRQQY | NTILSDMDKI | 
    100:
    YSTAKVCLDN | GTCWDLEPDI | SDIMATSRSY | KKLLYAWEGW | HNAAGNPLRA | 
    150:
    KYQEFVTLSN | EAYQMDGFED | TGSYWRSWYD | STTFEDDLEH | LYNQLEPLYL | 
    200:
    NLHAFVRRKL | YDRYGPKYIN | LKGPIPAHLL | GNMWAQQWNN | IYDLMVPYPD | 
    250:
    KPNLDVTNTM | VNQGWNATHM | FRVSEEFFTS | LGLLEMPPEF | WEKSMLEKPA | 
    300:
    DGREVVCHAS | AWDFYNRKDF | RIKQCTTVTM | EQLFTVHHEM | GHVQYYLQYK | 
    350:
    DQPVSFRGGA | NPGFHEAIGD | VLSLSVSTPS | HLQKIGLLSS | AVEDEESNIN | 
    400:
    YLLKMALEKI | AFLPFGYLID | QWRWNVFSGR | TPPSRYNYDW | WYLRTKYQGI | 
    450:
    CAPVSRNESN | FDPGAKYHIP | GNTPYIRYFV | SFILQFQFHK | ALCQAANHTG | 
    500:
    PLHTCDIYMS | KEAGAKLREV | LKAGSSKSWQ | EILFNLTGTD | KMDAGALLEY | 
    550:
    FSPVTTWLQE | QNNKTNEVLG | WPEFDWRSPI | PEGYPEGIDK | IVDEAQAKEF | 
    600:
    LSEYNSTAEV | VWNAYTEASW | EYNTNITDHN | KEVMLEKNLA | MSKHTIEYGM | 
    650:
    RARQFDPSDF | QDETVTRILN | KLSVLERAAL | PEDELKEYNT | LLSDMETTYS | 
    700:
    VAKVCRENNT | FHPLDPDLTD | ILATSRDYNE | LLFAWKGWWD | ASGAKIKDKY | 
    750:
    KRYVELSNKA | AVLNGYTDNG | AYWRSLYETP | TFEEDLERLY | LQLQPLYLNL | 
    800:
    HAYVRRALYN | KYGAEHISLK | GPIPAHLLGN | MWAQSWSNIF | DLVMPFPDAT | 
    850:
    KVDATPAMKQ | QGWTPKMMFE | ESDRFFTSLG | LIPMPQEFWD | KSMIEKPADG | 
    900:
    REVVCHASAW | DFYNRKDFRI | KQCTVVNMDD | LITVHHEMGH | VQYFLQYMDQ | 
    950:
    PISFRDGANP | GFHEAIGDVM | ALSVSTPKHL | HSINLLDQVT | ENEESDINYL | 
    1000:
    MSIALDKIAF | LPFGYLMDQW | RWKVFDGRIK | EDEYNQQWWN | LRLKYQGLCP | 
    1050:
    PVPRSEDDFD | PGAKFHIPAN | VPYIRYFVSF | VIQFQFHQAL | CKAAGHTGPL | 
    1100:
    HTCDIYQSKE | AGKLLGDAMK | LGFSKPWPEA | MQLITGQPNM | SAEALMSYFE | 
    1150:
    PLMTWLVKKN | TENGEVLGWP | EYSWTPYAVT | EFHAATDTAD | FLGMSVGTKQ | 
    1193:
    ATAGAWVLLA | LALVFLITSI | FLGVKLFSSR | RKAFKSSSEM | ELK
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    UniGene:
    String:
    Pfam:
    SMR:
    Uniprot:
     
    FOR
    ELISA Kit for Chicken ACE
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    E0004h
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    96T
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    ELISA Kit for Chicken ACE
    Cat.:
    E0004m
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    96T
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    ELISA Kit for Chicken ACE
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    E0004r
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    ELISA Kit for Chicken ACE
    Cat.:
    E0004b
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    96T
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    ELISA Kit for Chicken ACE
    MSDS:
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    ELISA Kit for Chicken ACE
    Cat.:
    E0004c
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    Packing:
    96T
    CLIA Kit for Chicken ACE
    MSDS:
    Please sign in first.
    CLIA Kit for Chicken ACE
    Cat.:
    U0004h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Chicken ACE
    Cat.:
    U0004m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Chicken ACE
    Cat.:
    U0004r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Chicken ACE
    Cat.:
    U0004c
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    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Chicken ACE
    Cat.:
    U0004b
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    MSDS:
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    Packing:
    96T
    Polyclonal Antibody for Chicken ACE
    Polyclonal Antibody for Chicken ACE
    Cat.:
    P0004Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Chicken ACE
    Polyclonal Antibody for Chicken ACE
    Cat.:
    P0004Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Chicken ACE
    Cat.:
    P0004Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Chicken ACE
    Monoclonal Antibody for Chicken ACE
    Monoclonal Antibody for Chicken ACE
    Monoclonal Antibody for Chicken ACE
    Monoclonal Antibody for Chicken ACE
    Monoclonal Antibody for Chicken ACE
    Monoclonal Antibody for Chicken ACE
    Protein for Chicken ACE
    Protein for Chicken ACE
    Protein for Chicken ACE
    Protein for Chicken ACE
    Protein for Chicken ACE
    Protein for Chicken ACE

    R&D Technical Data
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    s

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    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤4.0%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.9%



     

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    Recovery

    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery  Range (%)

    Serum

    101

    95-107

    Plasma

    99

    93-102

     

     

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    Linearity

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    Saliva(n=3)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    93-104%

    91-101%

    95-102%

    96-109%

    Saliva(n=3)

    90-99%

    93- 99%

    89-97%

    92-106%

    heparin plasma(n=5)

    94-103%

     

    96-110%

    99-111%

    94-110%

     

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    References
    1. 1.
      "Chicken lacks the testis specific isozyme of angiotensin converting enzyme found in mammals."
      Esther C.R. , Thomas K.E. , Bernstein K.E.
      Biochem. Biophys. Res. Commun.205:1916-1921(1994) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      tissue: Lung.
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