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Index > Protein center > Ace(Gene name) > Mouse
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  • Ace (Gene name),
  • Angiotensin-converting enzyme (Protein name ),  ACE_MOUSE from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    Ace(Dcp1);
    Protein name:
    Angiotensin-converting enzyme(ACE);
    Alternative:
    Dipeptidyl carboxypeptidase I;Kininase II;
    CD:
    CD143;
    Organism:
    Mouse (Mus musculus). 
    General Annotation
    Sub Unit:
    N/A
    Function:
    Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. This GPIase activity seems to be crucial for the egg-binding ability of the sperm.
    Subcellular Location:
    Cell membrane Single-pass type I membrane protein
    Protein Attributes:
    Sequence length:
    1312
    Sequence:
    50:
    MGAASGQRGR | WPLSPPLLML | SLLVLLLQPS | PAPALDPGLQ | PGNFSPDEAG | 
    100:
    AQLFAESYNS | SAEVVMFQST | VASWAHDTNI | TEENARRQEE | AALVSQEFAE | 
    150:
    VWGKKAKELY | ESIWQNFTDS | KLRRIIGSIR | TLGPANLPLA | QRQQYNSLLS | 
    200:
    NMSRIYSTGK | VCFPNKTATC | WSLDPELTNI | LASSRSYAKL | LFAWEGWHDA | 
    250:
    VGIPLKPLYQ | DFTAISNEAY | RQDDFSDTGA | FWRSWYESPS | FEESLEHIYH | 
    300:
    QLEPLYLNLH | AYVRRALHRR | YGDKYVNLRG | PIPAHLLGDM | WAQSWENIYD | 
    350:
    MVVPFPDKPN | LDVTSTMVQK | GWNATHMFRV | SEEFFTSLGL | SPMPPEFWAE | 
    400:
    SMLEKPTDGR | EVVCHASAWD | FYNRKDFRIK | QCTRVTMEQL | ATVHHEMGHV | 
    450:
    QYYLQYKDLH | VSLRRGANPG | FHEAIGDVLA | LSVSTPAHLH | KIGLLDHVTN | 
    500:
    DIESDINYLL | KMALEKIAFL | PFGYLVDQWR | WGVFSGRTPP | SRYNFDWWYL | 
    550:
    RTKYQGICPP | VARNETHFDA | GAKFHIPNVT | PYIRYFVSFV | LQFQFHQALC | 
    600:
    KEAGHQGPLH | QCDIYQSAQA | GAKLKQVLQA | GCSRPWQEVL | KDLVGSDALD | 
    650:
    AKALLEYFQP | VSQWLEEQNQ | RNGEVLGWPE | NQWRPPLPDN | YPEGIDLETD | 
    700:
    EAKADRFVEE | YDRTAQVLLN | EYAEANWQYN | TNITIEGSKI | LLEKSTEVSN | 
    750:
    HTLKYGTRAK | TFDVSNFQNS | SIKRIIKKLQ | NLDRAVLPPK | ELEEYNQILL | 
    800:
    DMETTYSLSN | ICYTNGTCMP | LEPDLTNMMA | TSRKYEELLW | AWKSWRDKVG | 
    850:
    RAILPFFPKY | VEFSNKIAKL | NGYTDAGDSW | RSLYESDNLE | QDLEKLYQEL | 
    900:
    QPLYLNLHAY | VRRSLHRHYG | SEYINLDGPI | PAHLLGNMWA | QTWSNIYDLV | 
    950:
    APFPSAPNID | ATEAMIKQGW | TPRRIFKEAD | NFFTSLGLLP | VPPEFWNKSM | 
    1000:
    LEKPTDGREV | VCHPSAWDFY | NGKDFRIKQC | TSVNMEDLVI | AHHEMGHIQY | 
    1050:
    FMQYKDLPVT | FREGANPGFH | EAIGDIMALS | VSTPKHLYSL | NLLSTEGSGY | 
    1100:
    EYDINFLMKM | ALDKIAFIPF | SYLIDQWRWR | VFDGSITKEN | YNQEWWSLRL | 
    1150:
    KYQGLCPPVP | RSQGDFDPGS | KFHVPANVPY | VRYFVSFIIQ | FQFHEALCRA | 
    1200:
    AGHTGPLHKC | DIYQSKEAGK | LLADAMKLGY | SKPWPEAMKL | ITGQPNMSAS | 
    1250:
    AMMNYFKPLT | EWLVTENRRH | GETLGWPEYN | WAPNTARAEG | STAESNRVNF | 
    1300:
    LGLYLEPQQA | RVGQWVLLFL | GVALLVATVG | LAHRLYNIRN | HHSLRRPHRG | 
    1312:
    PQFGSEVELR | HS
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
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    Related Databases
    SMR:
    UniGene:
    String:
    KEGG:
    Pfam:
    Uniprot:
     
    FOR
    ELISA Kit for Mouse ACE
    Cat.:
    E0004h
    Price:
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    MSDS:
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    Packing:
    96T
    Range:
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    ELISA Kit for Mouse ACE
    Cat.:
    E0004m
    Price:
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    MSDS:
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    Packing:
    96T
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    ELISA Kit for Mouse ACE
    Cat.:
    E0004r
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    MSDS:
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    96T
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    ELISA Kit for Mouse ACE
    Cat.:
    E0004b
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    MSDS:
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    Packing:
    96T
    Range:
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    ELISA Kit for Mouse ACE
    MSDS:
    Please sign in first.
    ELISA Kit for Mouse ACE
    Cat.:
    E0004c
    Price:
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Mouse ACE
    MSDS:
    Please sign in first.
    CLIA Kit for Mouse ACE
    Cat.:
    U0004h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Mouse ACE
    Cat.:
    U0004m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Mouse ACE
    Cat.:
    U0004r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Mouse ACE
    Cat.:
    U0004c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Mouse ACE
    Cat.:
    U0004b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Mouse ACE
    Polyclonal Antibody for Mouse ACE
    Cat.:
    P0004Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse ACE
    Polyclonal Antibody for Mouse ACE
    Cat.:
    P0004Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse ACE
    Cat.:
    P0004Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse ACE
    Monoclonal Antibody for Mouse ACE
    Monoclonal Antibody for Mouse ACE
    Monoclonal Antibody for Mouse ACE
    Monoclonal Antibody for Mouse ACE
    Monoclonal Antibody for Mouse ACE
    Monoclonal Antibody for Mouse ACE
    Protein for Mouse ACE
    Protein for Mouse ACE
    Protein for Mouse ACE
    Protein for Mouse ACE
    Protein for Mouse ACE
    Protein for Mouse ACE

    R&D Technical Data
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    s

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    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤4.0%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.9%



     

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    Recovery

    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery  Range (%)

    Serum

    101

    95-107

    Plasma

    99

    93-102

     

     

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    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    Linearity

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    Saliva(n=3)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    93-104%

    91-101%

    95-102%

    96-109%

    Saliva(n=3)

    90-99%

    93- 99%

    89-97%

    92-106%

    heparin plasma(n=5)

    94-103%

     

    96-110%

    99-111%

    94-110%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    References
    1. 1.
      "Mouse angiotensin-converting enzyme is a protein composed of two homologous domains."
      Bernstein K.E. , Martin B.M. , Edwards A.S. , Bernstein E.A.
      J. Biol. Chem.264:11945-11951(1989) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SOMATIC)
    2. 2.
      "Transcription of testicular angiotensin-converting enzyme (ACE) is initiated within the 12th intron of the somatic ACE gene."
      Howard T.E. , Shai S.-Y. , Langford K.G. , Martin B.M. , Bernstein K.E.
      Mol. Cell. Biol.10:4294-4302(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM TESTIS-SPECIFIC);PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    3. 3.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SOMATIC)
      strain: FVB/N.
      tissue: Mammary gland.
    4. 4.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-332 (ISOFORM SOMATIC);PARTIAL PROTEIN SEQUENCE
    5. 5.
      "Partial protein sequence of mouse and bovine kidney angiotensin converting enzyme."
      Bernstein K.E. , Martin B.M. , Striker L. , Striker G.
      Kidney Int.33:652-655(1988) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 35-54
      tissue: Kidney.
    6. 6.
      "Male-female differences in fertility and blood pressure in ACE-deficient mice."
      Krege J.H. , John S.W. , Langenbach L.L. , Hodgin J.B. , Hagaman J.R. , Bachman E.S. , Jennette J.C. , O'Brien D.A. , Smithies O.
      Nature375:146-148(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    7. 7.
      "Mice lacking angiotensin-converting enzyme have low blood pressure, renal pathology, and reduced male fertility."
      Esther C.R. Jr. , Howard T.E. , Marino E.M. , Goddard J.M. , Capecchi M.R. , Bernstein K.E.
      Lab. Invest.74:953-965(1996) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;DISRUPTION PHENOTYPE
    8. 8.
      "Angiotensin-converting enzyme is a GPI-anchored protein releasing factor crucial for fertilization."
      Kondoh G. , Tojo H. , Nakatani Y. , Komazawa N. , Murata C. , Yamagata K. , Maeda Y. , Kinoshita T. , Okabe M. , Taguchi R. , Takeda J.
      Nat. Med.11:160-166(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;ENZYME REGULATION;MUTAGENESIS OF HIS-993; GLU-994 AND HIS-997
    9. 9.
      "Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
      Ghesquiere B. , Van Damme J. , Martens L. , Vandekerckhove J. , Gevaert K.
      J. Proteome Res.5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-151
      strain: C57BL/6.
      tissue: Plasma.
    10. 10.
      "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
      Munton R.P. , Tweedie-Cullen R. , Livingstone-Zatchej M. , Weinandy F. , Waidelich M. , Longo D. , Gehrig P. , Potthast F. , Rutishauser D. , Gerrits B. , Panse C. , Schlapbach R. , Mansuy I.M.
      Mol. Cell. Proteomics6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Brain cortex.
    11. 11.
      "Angiotensin II regulates ACE and ACE2 in neurons through p38 mitogen-activated protein kinase and extracellular signal-regulated kinase 1/2 signaling."
      Xiao L. , Haack K.K. , Zucker I.H.
      Am. J. Physiol.304:C1073-C1079(2013) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION
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