Egr1 ELISA Kit for Mouse(Mus musculus) | EIAab.comEgr1 ELISA Kit for Mouse(Mus musculus)

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Egr1 (Gene name),

Early growth response protein 1 (Protein name ), EGR1_MOUSE from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

Egr1(Egr-1;Krox-24);

Protein name:

Early growth response protein 1(EGR-1);

Alternative:

Transcription factor Zif268;Nerve growth factor-induced protein A(NGFI-A);Zinc finger protein Krox-24;

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

N/A

Function:

Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.

Subcellular Location:

Nucleus

Protein Attributes:

Sequence length:

533

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

533:

SSFPSAGVSS | SFSTSTGLSD | MTATFSPRTI | EIC

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

SMR:

P08046

UniGene:

Mm.181959

String:

P08046

Pfam:

PF11914

KEGG:

mmu:13653

Uniprot:

P08046

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ELISA Kit for Mouse EGR-1

Cat.:

E0416h

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96T

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ELISA Kit for Mouse EGR-1

Cat.:

E0416r

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96T

ELISA Kit for Mouse EGR-1

Cat.:

E0416b

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96T

ELISA Kit for Mouse EGR-1

Cat.:

E0416m

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96T

ELISA Kit for Mouse EGR-1

Cat.:

E0416c

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96T

CLIA Kit for Mouse EGR-1

Cat.:

U0416m

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96T

CLIA Kit for Mouse EGR-1

Cat.:

U0416c

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96T

CLIA Kit for Mouse EGR-1

Cat.:

U0416h

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Manual:

Looking for manual.

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96T

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CLIA Kit for Mouse EGR-1

Cat.:

U0416r

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96T

CLIA Kit for Mouse EGR-1

Cat.:

U0416b

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96T

Polyclonal Antibody for Mouse EGR-1

Cat.:

P0416Rb-r

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Packing:

40ug/0.2ml

Polyclonal Antibody for Mouse EGR-1

Cat.:

P0416Rb-h

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40ug/0.2ml

Polyclonal Antibody for Mouse EGR-1

Cat.:

P0416Rb-m

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40ug/0.2ml

Polyclonal Antibody for Mouse EGR-1

Monoclonal Antibody for Mouse EGR-1

Protein for Mouse EGR-1

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.9%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of EGR1 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	101	96-103
Plasma	100	98-102

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EGR1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-103%	89-98%	102-109%	100-109%
EDTA plasma(n=5)	93-99%	103-108%	94-98%	96-102%
heparin plasma(n=5)	98-104%	100-106%	95-104%	93-100%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"A zinc finger-encoding gene coregulated with c-fos during growth and differentiation, and after cellular depolarization."
Sukhatme V.P. , Cao X. , Chang L.C. , Tsai-Morris C.-H. , Stamenkovich D. , Ferreira P.C.P. , Cohen D.R. , Edwards S.A. , Shows T.B. , Curran T. , le Beau M.M. , Adamson E.D.
Cell53:37-43(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
2.

"A gene activated in mouse 3T3 cells by serum growth factors encodes a protein with 'zinc finger' sequences."
Christy B.A. , Lau L.F. , Nathans D.
Proc. Natl. Acad. Sci. U.S.A.85:7857-7861(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
3.

"Structure, chromosome mapping and regulation of the mouse zinc-finger gene Krox-24; evidence for a common regulatory pathway for immediate-early serum-response genes."
Janssen-Timmen U. , Lemaire P. , Mattei M.-G. , Revelant O. , Charnay P.
Gene80:325-336(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
4.

"Two mouse genes encoding potential transcription factors with identical DNA-binding domains are activated by growth factors in cultured cells."
Lemaire P. , Revelant O. , Bravo R. , Charnay P.
Proc. Natl. Acad. Sci. U.S.A.85:4691-4695(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 50-533
5.

"In vivo mutational analysis of the NGFI-A zinc fingers."
Wilson T.E. , Day M.L. , Pexton T. , Padgett K.A. , Johnston M. , Milbrandt J.
J. Biol. Chem.267:3718-3724(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MUTAGENESIS OF ZINC-FINGERS
6.

"Zinc finger-DNA recognition: crystal structure of a Zif268-DNA complex at 2.1 A."
Pavletich N.P. , Pabo C.O.
Science252:809-817(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 333-421
7.

"High-resolution structures of variant Zif268-DNA complexes: implications for understanding zinc finger-DNA recognition."
Elrod-Erickson M. , Benson T.E. , Pabo C.O.
Structure6:451-464(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 333-421 WITH VARIATIONS

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