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Index > Protein center > Egr1(Gene name) > Rat
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  • Egr1 (Gene name),
  • Early growth response protein 1 (Protein name ),  EGR1_RAT from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    Egr1;
    Protein name:
    Early growth response protein 1(EGR-1);
    Alternative:
    Transcription factor Zif268;Nerve growth factor-induced protein A(NGFI-A);Zinc finger protein Krox-24;
    Organism:
    Rat (Rattus norvegicus). 
    General Annotation
    Sub Unit:
    N/A
    Function:
    Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.
    Subcellular Location:
    Nucleus
    Protein Attributes:
    Sequence length:
    508
    Sequence:
    50:
    MDNYPKLEEM | MLLSNGAPQF | LGAAGTPEGS | GGNNSSSSSS | SSSGGGGGGG | 
    100:
    SNSGSSAFNP | QGEPSEQPYE | HLTTESFSDI | ALNNEKALVE | TSYPSQTTRL | 
    150:
    PPITYTGRFS | LEPAPNSGNT | LWPEPLFSLV | SGLVSMTNPP | TSSSSAPSPA | 
    200:
    ASSSSSASQS | PPLSCAVPSN | DSSPIYSAAP | TFPTPNTDIF | PEPQSQAFPG | 
    250:
    SAGTALQYPP | PAYPATKGGF | QVPMIPDYLF | PQQQGDLSLG | TPDQKPFQGL | 
    300:
    ENRTQQPSLT | PLSTIKAFAT | QSGSQDLKAL | NNTYQSQLIK | PSRMRKYPNR | 
    350:
    PSKTPPHERP | YACPVESCDR | RFSRSDELTR | HIRIHTGQKP | FQCRICMRNF | 
    400:
    SRSDHLTTHI | RTHTGEKPFA | CDICGRKFAR | SDERKRHTKI | HLRQKDKKAD | 
    450:
    KSVVASSAAS | SLSSYPSPVA | TSYPSPATTS | FPSPVPTSYS | SPGSSTYPSP | 
    500:
    AHSGFPSPSV | ATTYASVPPA | FPAQVSTFQS | AGVSNSFSTS | TGLSDMTATF | 
    508:
    SPRTIEIC
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
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    Related Databases
    String:
    SMR:
    KEGG:
    Pfam:
    UniGene:
    Uniprot:
     
    FOR
    ELISA Kit for Rat EGR-1
    Cat.:
    E0416h
    Price:
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    MSDS:
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    Packing:
    96T
    Range:
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    ELISA Kit for Rat EGR-1
    Cat.:
    E0416r
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Rat EGR-1
    Cat.:
    E0416b
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Rat EGR-1
    Cat.:
    E0416m
    Price:
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    MSDS:
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    Packing:
    96T
    ELISA Kit for Rat EGR-1
    Cat.:
    E0416c
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat EGR-1
    Cat.:
    U0416m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat EGR-1
    Cat.:
    U0416c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat EGR-1
    Cat.:
    U0416h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat EGR-1
    Cat.:
    U0416r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat EGR-1
    Cat.:
    U0416b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Rat EGR-1
    Polyclonal Antibody for Rat EGR-1
    Cat.:
    P0416Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat EGR-1
    Cat.:
    P0416Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat EGR-1
    Cat.:
    P0416Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat EGR-1
    Monoclonal Antibody for Rat EGR-1
    Monoclonal Antibody for Rat EGR-1
    Monoclonal Antibody for Rat EGR-1
    Monoclonal Antibody for Rat EGR-1
    Monoclonal Antibody for Rat EGR-1
    Protein for Rat EGR-1
    Protein for Rat EGR-1
    Protein for Rat EGR-1
    Protein for Rat EGR-1
    Protein for Rat EGR-1

    R&D Technical Data
    s
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    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.6%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.9%

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    Recovery

    Recovery was determined by spiking various levels of EGR1 into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    101

    96-103

    Plasma

    100

    98-102

     

     

     

     

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    Linearity

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EGR1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    96-103%

    89-98%

    102-109%

    100-109%

    EDTA plasma(n=5)

    93-99%

    103-108%

    94-98%

    96-102%

    heparin plasma(n=5)

    98-104%

     

    100-106%

    95-104%

    93-100%

     

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    References
    1. 1.
      "A nerve growth factor-induced gene encodes a possible transcriptional regulatory factor."
      Milbrandt J.
      Science238:797-799(1987) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    2. 2.
      "Structure of the NGFI-A gene and detection of upstream sequences responsible for its transcriptional induction by nerve growth factor."
      Changelian P.S. , Feng P. , King T.C. , Milbrandt J.
      Proc. Natl. Acad. Sci. U.S.A.86:377-381(1989) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    3. 3.
      "Cloning and sequencing of cDNA encoding EGR1 from rat."
      Ju S.K.
      Submitted (2004-02) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      strain: Sprague-Dawley.
      tissue: Midbrain.
    4. 4.
      "Transcriptional activity of the zinc finger protein NGFI-A is influenced by its interaction with a cellular factor."
      Russo M.W. , Matheny C. , Milbrandt J.
      Mol. Cell. Biol.13:6858-6865(1993) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MUTAGENESIS OF ILE-265
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