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Index > Protein center > Ang(Gene name) > Mouse
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  • Ang (Gene name),
  • Angiogenin (Protein name ),  ANGI_MOUSE from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    Ang(Ang1;Rnase5;Rnase5a);
    Protein name:
    Angiogenin;
    Alternative:
    Ribonuclease 5(RNase 5);Angiogenin-1;
    Organism:
    Mouse (Mus musculus). 
    General Annotation
    Sub Unit:
    Interacts with and forms a tight 1:1 complex with RNH1. Dimerization of two such complexes may occur.
    Function:
    May function as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo.
    Subcellular Location:
    Secreted
    Protein Attributes:
    Sequence length:
    145
    Sequence:
    50:
    MAISPGPLFL | IFVLGLVVIP | PTLAQDDSRY | TKFLTQHHDA | KPKGRDDRYC | 
    100:
    ERMMKRRSLT | SPCKDVNTFI | HGNKSNIKAI | CGANGSPYRE | NLRMSKSPFQ | 
    145:
    VTTCKHTGGS | PRPPCQYRAS | AGFRHVVIAC | ENGLPVHFDE | SFFSL
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
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    Related Databases
    UniGene:
    String:
    SMR:
    KEGG:
    Pfam:
    Uniprot:
     
    FOR
    ELISA Kit for Mouse Angiogenin
    Cat.:
    E0007h
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    MSDS:
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    96T
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    ELISA Kit for Mouse Angiogenin
    Cat.:
    E0007m
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    MSDS:
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    Packing:
    96T
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    ELISA Kit for Mouse Angiogenin
    MSDS:
    Please sign in first.
    ELISA Kit for Mouse Angiogenin
    Cat.:
    E0007p
    Price:
    Please sign in first.
    MSDS:
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    Packing:
    96T
    Range:
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    ELISA Kit for Mouse Angiogenin
    Cat.:
    E0007c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Mouse Angiogenin
    Cat.:
    U0007p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Mouse Angiogenin
    MSDS:
    Please sign in first.
    CLIA Kit for Mouse Angiogenin
    Cat.:
    U0007h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Mouse Angiogenin
    Cat.:
    U0007m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Mouse Angiogenin
    Cat.:
    U0007c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Mouse Angiogenin
    Polyclonal Antibody for Mouse Angiogenin
    Polyclonal Antibody for Mouse Angiogenin
    Cat.:
    P0007Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse Angiogenin
    Cat.:
    P0007Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse Angiogenin
    Monoclonal Antibody for Mouse Angiogenin
    Monoclonal Antibody for Mouse Angiogenin
    Monoclonal Antibody for Mouse Angiogenin
    Monoclonal Antibody for Mouse Angiogenin
    Monoclonal Antibody for Mouse Angiogenin
    Protein for Mouse Angiogenin
    Protein for Mouse Angiogenin
    Protein for Mouse Angiogenin
    Protein for Mouse Angiogenin
    Protein for Mouse Angiogenin

    R&D Technical Data

          12% SDS-PAGE Analysis

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    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤ 5.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤ 9.2%

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    Recovery

    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    95

    91-101

    Plasma

    94

    90-99

     

     

     

     

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    Linearity

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    89-99%

    91-102%

    93-103%

    90-104%

    EDTA plasma(n=5)

    89-98%

    90-103%

    91-97%

    92-102%

    heparin plasma(n=5)

    90-101%

     

    92-105%

    94-107%

    91-106%

     

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    References
    1. 1.
      "Isolation and sequencing of mouse angiogenin DNA."
      Bond M.D. , Vallee B.L.
      Biochem. Biophys. Res. Commun.171:988-995(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    2. 2.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      strain: FVB/N.
      tissue: Liver.
    3. 3.
      "Characterization and sequencing of rabbit, pig and mouse angiogenins: discernment of functionally important residues and regions."
      Bond M.D. , Strydom D.J. , Vallee B.L.
      Biochim. Biophys. Acta1162:177-186(1993) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PARTIAL PROTEIN SEQUENCE
      tissue: Serum.
    4. 4.
      "Stress induces tRNA cleavage by angiogenin in mammalian cells."
      Fu H. , Feng J. , Liu Q. , Sun F. , Tie Y. , Zhu J. , Xing R. , Sun Z. , Zheng X.
      FEBS Lett.583:437-442(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    5. 5.
      "Structure of murine angiogenin: features of the substrate- and cell-binding regions and prospects for inhibitor-binding studies."
      Holloway D.E. , Chavali G.B. , Hares M.C. , Subramanian V. , Acharya K.R.
      Acta Crystallogr. D61:1568-1578(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 25-145;DISULFIDE BONDS
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