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Index > Protein center > BDNF(Gene name) > Human
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  • BDNF (Gene name),
  • Brain-derived neurotrophic factor (Protein name ),  BDNF_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Related Databases
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  • Gene name:
    BDNF;
    Protein name:
    Brain-derived neurotrophic factor(BDNF);
    Alternative:
    Abrineurin;
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Monomers and homodimers. Binds to NTRK2/TRKB.
    Function:
    During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
    Subcellular Location:
    Secreted
    Protein Attributes:
    Sequence length:
    247
    Sequence:
    50:
    MTILFLTMVI | SYFGCMKAAP | MKEANIRGQG | GLAYPGVRTH | GTLESVNGPK | 
    100:
    AGSRGLTSLA | DTFEHVIEEL | LDEDQKVRPN | EENNKDADLY | TSRVMLSSQV | 
    150:
    PLEPPLLFLL | EEYKNYLDAA | NMSMRVRRHS | DPARRGELSV | CDSISEWVTA | 
    200:
    ADKKTAVDMS | GGTVTVLEKV | PVSKGQLKQY | FYETKCNPMG | YTKEGCRGID | 
    247:
    KRHWNSQCRT | TQSYVRALTM | DSKKRIGWRF | IRIDTSCVCT | LTIKRGR
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    String:
    SMR:
    UniGene:
    Pfam:
    KEGG:
    MIM:
    Uniprot:
     
    FOR
    ELISA Kit for Human BDNF
    Cat.:
    E0011h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human BDNF
    Cat.:
    E0011m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human BDNF
    Cat.:
    E0011r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human BDNF
    Cat.:
    E0011d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human BDNF
    Cat.:
    E0011c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human BDNF
    Cat.:
    E0011b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Human BDNF
    Cat.:
    E0011p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human BDNF
    Cat.:
    U0011p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human BDNF
    Cat.:
    U0011c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human BDNF
    Cat.:
    U0011h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human BDNF
    Cat.:
    U0011m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human BDNF
    Cat.:
    U0011r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human BDNF
    Cat.:
    U0011b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human BDNF
    Cat.:
    U0011d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Human BDNF
    Polyclonal Antibody for Human BDNF
    Polyclonal Antibody for Human BDNF
    Polyclonal Antibody for Human BDNF
    Cat.:
    P0011Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human BDNF
    Cat.:
    P0011Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human BDNF
    Polyclonal Antibody for Human BDNF
    Cat.:
    P0011Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Human BDNF
    Monoclonal Antibody for Human BDNF
    Monoclonal Antibody for Human BDNF
    Monoclonal Antibody for Human BDNF
    Monoclonal Antibody for Human BDNF
    Monoclonal Antibody for Human BDNF
    Monoclonal Antibody for Human BDNF
    Protein for Human BDNF
    Protein for Human BDNF
    Protein for Human BDNF
    Protein for Human BDNF
    Protein for Human BDNF
    Protein for Human BDNF
    Protein for Human BDNF

    R&D Technical Data

    s

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    s

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    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤4.1%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.4%

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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤4.4%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.1%

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    Recovery

    Recovery was determined by spiking various levels of BDNF into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    93

    91-98

    Plasma

    94

    93-100

     

     

     

     

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    Recovery was determined by spiking various levels of BDNF into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    94-103

    Plasma

    98

    96-100

     

     

     

     

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    Linearity

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BDNF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-96%

    90-100%

    95-99%

    92-96%

    EDTA plasma(n=5)

    96-103%

    93-96%

    96-98%

    93-102%

    heparin plasma(n=5)

    89-104%

     

    91-96%

    89-92%

    87-97%

     

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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BDNF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    96-101%

    93-99%

    95-105%

    92-96%

    EDTA plasma(n=5)

    99-104%

    94-106%

    99-110%

    96-99%

    heparin plasma(n=5)

    95-102%

     

    97-102%

    101-109%

    91-96%

     

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    References
    1. 1.
      "Molecular cloning of a human gene that is a member of the nerve growth factor family."
      Jones K.R. , Reichardt L.F.
      Proc. Natl. Acad. Sci. U.S.A.87:8060-8064(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];ALTERNATIVE SPLICING (ISOFORM 1)
    2. 2.
      "Human and rat brain-derived neurotrophic factor and neurotrophin-3: gene structures, distributions, and chromosomal localizations."
      Maisonpierre P.C. , le Beau M.M. , Espinosa R. III , Ip N.Y. , Belluscio L. , de la Monte S.M. , Squinto S. , Furth M.E. , Yancopoulos G.D.
      Genomics10:558-568(1991) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1)
    3. 3.
      "Characterization of the 5'-flanking region of the human brain-derived neurotrophic factor gene."
      Shintani A. , Ono Y. , Kaisho Y. , Igarashi K.
      Biochem. Biophys. Res. Commun.182:325-332(1992) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
    4. 4.
      "Human brain derived neurotrophic factor (BDNF) genes, splicing patterns, and assessments of associations with substance abuse and Parkinson's Disease."
      Liu Q.-R. , Walther D. , Drgon T. , Polesskaya O. , Lesnick T.G. , Strain K.J. , de Andrade M. , Bower J.H. , Maraganore D.M. , Uhl G.R.
      Am. J. Med. Genet. B Neuropsychiatr. Genet.134:93-103(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1; 2 AND 3);ALTERNATIVE SPLICING
    5. 5.
      "Dissecting the human BDNF locus: bidirectional transcription, complex splicing, and multiple promoters."
      Pruunsild P. , Kazantseva A. , Aid T. , Palm K. , Timmusk T.
      Genomics90:397-406(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3; 4 AND 5);ALTERNATIVE SPLICING;ALTERNATIVE PROMOTER USAGE;TISSUE SPECIFICITY
      tissue: Brain.
    6. 6.
      "A cDNA clone of human brain-derived neurotrophic factor (HUMBDNFD)."
      Cheng Y. , Gu J.
      Submitted (1995-05) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
    7. 7.
      Wu J. , Zhang B. , Zhou Y. , Peng X. , Yuan J. , Qiang B.
      Submitted (2001-07) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
    8. 8.
      Perez-Pinera P. , Gonzalez-Martinez T. , Garcia-Suarez O. , Perez-Perez M. , Esteban I. , Monjil D. , Vega J.A.
      Submitted (2004-06) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);VARIANT MET-66
    9. 9.
      "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
      Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2)
      tissue: Brain.
    10. 10.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    11. 11.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    12. 12.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    13. 13.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
      tissue: Brain.
    14. 14.
      "Evolutionary studies of the nerve growth factor family reveal a novel member abundantly expressed in Xenopus ovary."
      Hallboeoek F. , Ibanez C.F. , Persson H.
      Neuron6:845-858(1991) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 185-227
      tissue: Leukocyte.
    15. 15.
      "Purification and identification of brain-derived neurotrophic factor from human serum."
      Rosenfeld R.D. , Zeni L. , Haniu M. , Talvenheimo J. , Radka S.F. , Bennett L. , Miller J.A. , Welcher A.A.
      Protein Expr. Purif.6:465-471(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 129-144
      tissue: Serum.
    16. 16.
      "Molecular phylogenetics and the origins of placental mammals."
      Murphy W.J. , Eizirik E. , Johnson W.E. , Zhang Y.-P. , Ryder O.A. , O'Brien S.J.
      Nature409:614-618(2001) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 12-197
    17. 17.
      "Biosynthesis and post-translational processing of the precursor to brain-derived neurotrophic factor."
      Mowla S.J. , Farhadi H.F. , Pareek S. , Atwal J.K. , Morris S.J. , Seidah N.G. , Murphy R.A.
      J. Biol. Chem.276:12660-12666(2001) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CHARACTERIZATION;MUTAGENESIS OF ARG-54
    18. 18.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: POLYMORPHISM;ASSOCIATION WITH BULIMIA NERVOSA
    19. 19.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INVOLVEMENT IN WAGRO SYNDROME
    20. 20.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ERRATUM
    21. 21.
      "Structure of the brain-derived neurotrophic factor/neurotrophin 3 heterodimer."
      Robinson R.C. , Radziejewski C. , Stuart D.I. , Jones E.Y.
      Biochemistry34:4139-4146(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS)
    22. 22.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT MET-66
    23. 23.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ERRATUM
    24. 24.
      "Idiopathic congenital central hypoventilation syndrome: evaluation of brain-derived neurotrophic factor genomic DNA sequence variation."
      Weese-Mayer D.E. , Bolk S. , Silvestri J.M. , Chakravarti A.
      Am. J. Med. Genet.107:306-310(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT CCHS ILE-2
    25. 25.
      "Sequence variants of the brain-derived neurotrophic factor (BDNF) gene are strongly associated with obsessive-compulsive disorder."
      Hall D. , Dhilla A. , Charalambous A. , Gogos J.A. , Karayiorgou M.
      Am. J. Hum. Genet.73:370-376(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT MET-66;ROLE OF VARIANT MET-66 IN OBSESSIVE-COMPULSIVE DISORDER
    26. 26.
      "The BDNF val66met polymorphism affects activity-dependent secretion of BDNF and human memory and hippocampal function."
      Egan M.F. , Kojima M. , Callicott J.H. , Goldberg T.E. , Kolachana B.S. , Bertolino A. , Zaitsev E. , Gold B. , Goldman D. , Dean M. , Lu B. , Weinberger D.R.
      Cell112:257-269(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT MET-66;CHARACTERIZATION OF VARIANT MET-66;ROLE IN EPISODIC MEMORY
    27. 27.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT MET-66
    28. 28.
      "Met66 in the brain-derived neurotrophic factor (BDNF) precursor is associated with anorexia nervosa restrictive type."
      Ribases M. , Gratacos M. , Armengol L. , de Cid R. , Badia A. , Jimenez L. , Solano R. , Vallejo J. , Fernandez F. , Estivill X.
      Mol. Psychiatry8:745-751(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT MET-66;ASSOCIATION OF VARIANT MET-66 WITH ANR
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