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Index > Protein center > FGF1(Gene name) > Bovine
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  • FGF1 (Gene name),
  • Heparin-binding growth factor 1 (Protein name ),  FGF1_BOVIN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Related Databases
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  • Gene name:
    FGF1(AFGF;FGF-1;FGFA;HBGF-1);
    Protein name:
    Heparin-binding growth factor 1(HBGF-1);
    Alternative:
    Acidic fibroblast growth factor(aFGF);Acidic eye-derived growth factor II(EDGF II);Prostatropin(HBGF-1);Endothelial cell growth factor beta and alpha chains(ECGF);Prostatropin;
    Organism:
    Bovine (Bos taurus). 
    General Annotation
    Sub Unit:
    Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP1. Part of a Cu(2+)-dependent multiprotein aggregate containing FGF1, S100A13 and SYT1. Interacts with S100A13. Interacts with FGFBP1 (By similarity). Interacts with SYT1.
    Function:
    Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.
    Subcellular Location:
    Secreted Cytoplasm Cytoplasm cell cortex Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1.
    Protein Attributes:
    Sequence length:
    155
    Sequence:
    50:
    MAEGETTTFT | ALTEKFNLPL | GNYKKPKLLY | CSNGGYFLRI | LPDGTVDGTK | 
    100:
    DRSDQHIQLQ | LCAESIGEVY | IKSTETGQFL | AMDTDGLLYG | SQTPNEECLF | 
    150:
    LERLEENHYN | TYISKKHAEK | HWFVGLKKNG | RSKLGPRTHF | GQKAILFLPL | 
    155:
    PVSSD
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
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    Related Databases
    SMR:
    String:
    Pfam:
    UniGene:
    Uniprot:
     
    FOR
    ELISA Kit for Bovine HBGF-1
    Cat.:
    E0032h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Bovine HBGF-1
    Cat.:
    E0032m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Bovine HBGF-1
    Cat.:
    E0032r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Bovine HBGF-1
    Cat.:
    E0032p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Bovine HBGF-1
    Cat.:
    E0032b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Bovine HBGF-1
    Cat.:
    E0032d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Bovine HBGF-1
    Cat.:
    E0032c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Bovine HBGF-1
    Cat.:
    U0032r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Bovine HBGF-1
    Cat.:
    U0032p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Bovine HBGF-1
    Cat.:
    U0032m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Bovine HBGF-1
    Cat.:
    U0032h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Bovine HBGF-1
    Cat.:
    U0032d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Bovine HBGF-1
    Cat.:
    U0032b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Bovine HBGF-1
    Cat.:
    U0032c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Bovine HBGF-1
    Polyclonal Antibody for Bovine HBGF-1
    Polyclonal Antibody for Bovine HBGF-1
    Cat.:
    P0032Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Bovine HBGF-1
    Polyclonal Antibody for Bovine HBGF-1
    Cat.:
    P0032Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Bovine HBGF-1
    Cat.:
    P0032Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Bovine HBGF-1
    Monoclonal Antibody for Bovine HBGF-1
    Monoclonal Antibody for Bovine HBGF-1
    Monoclonal Antibody for Bovine HBGF-1
    Monoclonal Antibody for Bovine HBGF-1
    Monoclonal Antibody for Bovine HBGF-1
    Monoclonal Antibody for Bovine HBGF-1
    Monoclonal Antibody for Bovine HBGF-1
    Protein for Bovine HBGF-1
    Protein for Bovine HBGF-1
    Protein for Bovine HBGF-1
    Protein for Bovine HBGF-1
    Protein for Bovine HBGF-1
    Protein for Bovine HBGF-1
    Protein for Bovine HBGF-1

    R&D Technical Data

    s

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    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤4.7%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤6.8%

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    Recovery

    Recovery was determined by spiking various levels of FGF1 into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    95

    93-97

    Plasma

    97

    96-102

     

     

     

     

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    Linearity

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FGF1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-100%

    97-104%

    95-98%

    91-96%

    EDTA plasma(n=5)

    89-93%

    102-105%

    94-97%

    93-98%

    heparin plasma(n=5)

    91-95%

     

    96-99%

    106-108%

    87-93%

     

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    References
    1. 1.
      "Nucleotide sequence of bovine acidic fibroblast growth factor cDNA."
      Halley C. , Courtois Y. , Laurent M.
      Nucleic Acids Res.16:10913-10913(1988) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      tissue: Retina.
    2. 2.
      "Characterization of a bovine acidic FGF cDNA clone and its expression in brain and retina."
      Alterio J. , Halley C. , Brou C. , Soussi T. , Courtois Y. , Laurent M.
      FEBS Lett.242:41-46(1988) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      tissue: Retina.
    3. 3.
      NIH - Mammalian Gene Collection (MGC) project
      Submitted (2005-08) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      strain: Hereford.
      tissue: Heart ventricle.
    4. 4.
      "Structural evidence that endothelial cell growth factor beta is the precursor of both endothelial cell growth factor alpha and acidic fibroblast growth factor."
      Burgess W.H. , Mehlman T. , Marshak D.R. , Fraser B.A. , Maciag T.
      Proc. Natl. Acad. Sci. U.S.A.83:7216-7220(1986) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 2-155;ACETYLATION AT ALA-2
    5. 5.
      "Complete primary structure of prostatropin, a prostate epithelial cell growth factor."
      Crabb J.W. , Armes L.G. , Carr S.A. , Johnson C.M. , Roberts G.D. , Bordoli R.S. , McKeehan W.L.
      Biochemistry25:4988-4993(1986) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 2-155
    6. 6.
      "Brain-derived acidic fibroblast growth factor: complete amino acid sequence and homologies."
      Gimenez-Gallego G. , Rodkey J. , Bennett C. , Rios-Candelore M. , Disalvo J. , Thomas K.
      Science230:1385-1388(1985) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 16-155
    7. 7.
      "Acidic fibroblast growth factor (FGF) from bovine brain: amino-terminal sequence and comparison with basic FGF."
      Boehlen P. , Esch F. , Baird A. , Gospodarowicz D.
      EMBO J.4:1951-1956(1985) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 16-44;AMINO-ACID COMPOSITION
    8. 8.
      "Nucleotide sequence of a bovine clone encoding the angiogenic protein, basic fibroblast growth factor."
      Abraham J.A. , Mergia A. , Whang J.L. , Tumolo A. , Friedman J. , Hjerrild K.A. , Gospodarowicz D. , Fiddes J.C.
      Science233:545-548(1986) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 16-56
    9. 9.
      "Isolation of heparin-binding growth factors from bovine, porcine and canine hearts."
      Quinkler W. , Maasberg M. , Bernotat-Danielowski S. , Luethe N. , Sharma H.S. , Schaper W.
      Eur. J. Biochem.181:67-73(1989) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 16-45
    10. 10.
      "Cloning of two different 5' untranslated exons of bovine acidic fibroblast growth factor by the single strand ligation to single-stranded cDNA methodology."
      Philippe J.-M. , Renaud F. , Desset S. , Laurent M. , Mallet J. , Courtois Y. , Edwards J.B.
      Biochem. Biophys. Res. Commun.188:843-850(1992) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-18
    11. 11.
      "The extravesicular domain of synaptotagmin-1 is released with the latent fibroblast growth factor-1 homodimer in response to heat shock."
      Tarantini F. , LaVallee T. , Jackson A. , Gamble S. , Mouta Carreira C. , Garfinkel S. , Burgess W.H. , Maciag T.
      J. Biol. Chem.273:22209-22216(1998) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH SYT1;FUNCTION;HEPARIN BINDING;SUBCELLULAR LOCATION
    12. 12.
      "Regulation of proliferation-survival decisions is controlled by FGF1 secretion in retinal pigmented epithelial cells."
      Bryckaert M. , Guillonneau X. , Hecquet C. , Perani P. , Courtois Y. , Mascarelli F.
      Oncogene19:4917-4929(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION
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      Zhu X. , Komiya H. , Chirino A. , Faham S. , Fox G.M. , Arakawa T. , Hsu B.T. , Rees D.C.
      Science251:90-93(1991) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS)
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