Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
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s
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Precision
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.3%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.5%
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Recovery
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Recovery was determined by spiking various levels of GDNF into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
105
102-108
Plasma
106
99-107
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Linearity
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GDNF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
102-106%
101-107%
96-103%
93-98%
EDTA plasma(n=5)
97-105%
104-108%
99-103%
89-96%
heparin plasma(n=5)
104-106%
107-110%
95-99%
100-105%
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[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];FUNCTION;HOMODIMERIZATION;DISULFIDE BONDS
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2);TISSUE SPECIFICITY
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2)
4.
"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L.
,
Schick M.
,
Neubert P.
,
Schatten R.
,
Henze S.
,
Korn B.
Submitted (2004-06) to the EMBL/GenBank/DDBJ databases
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-187 (ISOFORM 1)
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Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-88 (ISOFORM 2);NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-77;NUCLEOTIDE SEQUENCE [MRNA] OF 1-59 (ISOFORMS 3 AND 4)
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-50;SUBCELLULAR LOCATION;INDUCTION
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: PROTEIN SEQUENCE OF 87-122; 141-153; 173-184 AND 196-211;HOMODIMERIZATION;DISULFIDE BONDS
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Cited for: ALTERNATIVE SPLICING (ISOFORMS 1; 2; 3 AND 5);TISSUE SPECIFICITY
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) OF 78-211;GLYCOSYLATION AT ASN-126;DISULFIDE BONDS