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Index > Protein center > GZMB(Gene name) > Human
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  • GZMB (Gene name),
  • Granzyme B (Protein name ),  GRAB_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    GZMB(CGL1;CSPB;CTLA1;GRB);
    Protein name:
    Granzyme B;
    Alternative:
    CTLA-1;C11;Cytotoxic T-lymphocyte proteinase 2(Lymphocyte protease);Cathepsin G-like 1(CTSGL1);Granzyme-2;Fragmentin-2;SECT;Human lymphocyte protein(HLP);T-cell serine protease 1-3E;
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    N/A
    Function:
    This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
    Subcellular Location:
    Cytoplasmic granule Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.
    Protein Attributes:
    Sequence length:
    247
    Sequence:
    50:
    MQPILLLLAF | LLLPRADAGE | IIGGHEAKPH | SRPYMAYLMI | WDQKSLKRCG | 
    100:
    GFLIRDDFVL | TAAHCWGSSI | NVTLGAHNIK | EQEPTQQFIP | VKRPIPHPAY | 
    150:
    NPKNFSNDIM | LLQLERKAKR | TRAVQPLRLP | SNKAQVKPGQ | TCSVAGWGQT | 
    200:
    APLGKHSHTL | QEVKMTVQED | RKCESDLRHY | YDSTIELCVG | DPEIKKTSFK | 
    247:
    GDSGGPLVCN | KVAQGIVSYG | RNNGMPPRAC | TKVSSFVHWI | KKTMKRY
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    Pfam:
    UniGene:
    SMR:
    KEGG:
    String:
    MIM:
    Uniprot:
     
    FOR
    ELISA Kit for Human Granzyme B
    Cat.:
    E0600h
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    MSDS:
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    Packing:
    96T
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    ELISA Kit for Human Granzyme B
    Cat.:
    E0600r
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    MSDS:
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    Packing:
    96T
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    Please sign in first.
    ELISA Kit for Human Granzyme B
    Cat.:
    E0600m
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    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
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    CLIA Kit for Human Granzyme B
    Cat.:
    U0600m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human Granzyme B
    Cat.:
    U0600r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human Granzyme B
    Cat.:
    U0600h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    Polyclonal Antibody for Human Granzyme B
    Cat.:
    P0600Rb-r
    Price:
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Granzyme B
    Cat.:
    P0600Rb-h
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Granzyme B
    Cat.:
    P0600Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Human Granzyme B
    Monoclonal Antibody for Human Granzyme B
    Monoclonal Antibody for Human Granzyme B
    Protein for Human Granzyme B
    Protein for Human Granzyme B
    Protein for Human Granzyme B

    R&D Technical Data
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤4.1

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤6.9

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Recovery

    Recovery was determined by spiking various levels of Granzyme B into serum and plasma .

    Sample Type

    Average(%)

    Recovery  Range (%)

    Serum

    101

    85-109

    Plasma

    99

    94-110

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Linearity

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Granzyme B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    80-96%

    82-10%

    87-98%

    96-103%

    EDTA plasma(n=5)

    90-105%

    93-102%

    86-105%

    84-102%

    heparin plasma(n=5)

    85-99%

     

    85-94%

    84-101%

    85-101%

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    References
    1. 1.
      "Induction of mRNA for a serine protease and a beta-thromboglobulin-like protein in mitogen-stimulated human leukocytes."
      Schmid J. , Weissmann C.
      J. Immunol.139:250-256(1987) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];VARIANT GLN-55
    2. 2.
      "Structure and differential mechanisms of regulation of expression of a serine esterase gene in activated human T lymphocytes."
      Caputo A. , Fahey D. , Lloyd C. , Vozab R. , McCairns E. , Rowe P.B.
      J. Biol. Chem.263:6363-6369(1988) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];VARIANT ALA-94
    3. 3.
      "Molecular cloning of an inducible serine esterase gene from human cytotoxic lymphocytes."
      Trapani J.A. , Klein J.L. , White P.C. , Dupont B.
      Proc. Natl. Acad. Sci. U.S.A.85:6924-6928(1988) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];VARIANT GLN-55
    4. 4.
      "Genomic organization and chromosomal assignment for a serine protease gene (CSPB) expressed by human cytotoxic lymphocytes."
      Klein J.L. , Shows T.B. , Dupont B. , Trapani J.A.
      Genomics5:110-117(1989) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANT GLN-55
    5. 5.
      "Nucleotide sequence and genomic organization of a human T lymphocyte serine protease gene."
      Caputo A. , Sauer D.E. , Rowe P.B.
      J. Immunol.145:737-744(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANT GLN-55
    6. 6.
      "Structural organization of the hCTLA-1 gene encoding human granzyme B."
      Haddad P. , Clement M.-V. , Bernard O. , Larsen C.-J. , Degos L. , Sasportes M. , Mathieu-Mahul D.
      Gene87:265-271(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANTS GLN-55 AND ALA-94
    7. 7.
      "The DNA sequence and analysis of human chromosome 14."
      Heilig R. , Eckenberg R. , Petit J.-L. , Fonknechten N. , Da Silva C. , Cattolico L. , Levy M. , Barbe V. , De Berardinis V. , Ureta-Vidal A. , Pelletier E. , Vico V. , Anthouard V. , Rowen L. , Madan A. , Qin S. , Sun H. , Du H. , more...
      Nature421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    8. 8.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA];VARIANTS GLN-55 AND HIS-247
      tissue: Pancreas.
    9. 9.
      "Isolation of a cDNA clone encoding a novel form of granzyme B from human NK cells and mapping to chromosome 14."
      Dahl C.A. , Bach F.H. , Chan W. , Huebner K. , Russo G. , Croce C.M. , Herfurth T. , Cairns J.S.
      Hum. Genet.84:465-470(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-23
    10. 10.
      "Characterization of three serine esterases isolated from human IL-2 activated killer cells."
      Hameed A. , Lowrey D.M. , Lichtenheld M. , Podack E.R.
      J. Immunol.141:3142-3147(1988) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 21-40;CHARACTERIZATION
    11. 11.
      "Characterization of granzymes A and B isolated from granules of cloned human cytotoxic T lymphocytes."
      Kraehenbuhl O. , Rey C. , Jenne D.E. , Lanzavecchia A. , Groscurth P. , Carrel S. , Tschopp J.
      J. Immunol.141:3471-3477(1988) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 21-40;CHARACTERIZATION
    12. 12.
      "Human granzyme B degrades aggrecan proteoglycan in matrix synthesized by chondrocytes."
      Froelich C.J. , Zhang X. , Turbov J. , Hudig D. , Winkler U. , Hanna W.L.
      J. Immunol.151:7161-7171(1993) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 21-39;CATALYTIC ACTIVITY;ENZYME REGULATION;SUBCELLULAR LOCATION
      tissue: Lymphocyte.
    13. 13.
      "Human cytotoxic lymphocyte granzyme B. Its purification from granules and the characterization of substrate and inhibitor specificity."
      Poe M. , Blake J.T. , Boulton D.A. , Gammon M. , Sigal N.H. , Wu J.K. , Zweerink H.J.
      J. Biol. Chem.266:98-103(1991) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 21-38
    14. 14.
      "Signal peptide prediction based on analysis of experimentally verified cleavage sites."
      Zhang Z. , Henzel W.J.
      Protein Sci.13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 19-33
    15. 15.
      "Crystal structure of the caspase activator human granzyme B, a proteinase highly specific for an Asp-P1 residue."
      Estebanez-Perpina E. , Fuentes-Prior P. , Belorgey D. , Braun M. , Kiefersauer R. , Maskos K. , Huber R. , Rubin H. , Bode W.
      Biol. Chem.381:1203-1214(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF 21-247
    16. 16.
      "The three-dimensional structure of human granzyme B compared to caspase-3, key mediators of cell death with cleavage specificity for aspartic acid in P1."
      Rotonda J. , Garcia-Calvo M. , Bull H.G. , Geissler W.M. , McKeever B.M. , Willoughby C.A. , Thornberry N.A. , Becker J.W.
      Chem. Biol.8:357-368(2001) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 21-247
    17. 17.
      "Catalog of 680 variations among eight cytochrome p450 (CYP) genes, nine esterase genes, and two other genes in the Japanese population."
      Saito S. , Iida A. , Sekine A. , Kawauchi S. , Higuchi S. , Ogawa C. , Nakamura Y.
      J. Hum. Genet.48:249-270(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS GLN-55 AND HIS-247
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