IL10 ELISA Kit for Dog(Canis familiaris) | EIAab.comIL10 ELISA Kit for Dog(Canis familiaris)

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Index > Protein center > IL10(Gene name) > Dog

IL10 (Gene name),

Interleukin-10 (Protein name ), IL10_CANFA from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

IL10;

Protein name:

Interleukin-10(IL-10);

Alternative:

Cytokine synthesis inhibitory factor(CSIF);

Organism:

Dog (Canis familiarisCanis lupus familiaris).

General Annotation

Sub Unit:

Homodimer.

Function:

Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

Subcellular Location:

Secreted

Protein Attributes:

Sequence length:

181

Sequence:

50:

100:

150:

181:

KLQEKGVYKA | MSEFDIFINY | IETYMTMRMK | I

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

UniGene:

Cfa.38

KEGG:

cfa:403628

Pfam:

PF00726

String:

P48411

SMR:

P48411

Uniprot:

P48411

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ELISA Kit for Dog IL-10

Cat.:

E0056r

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96T

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ELISA Kit for Dog IL-10

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ELISA Kit for Dog IL-10

Cat.:

E0056p

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96T

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ELISA Kit for Dog IL-10

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E0056m

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ELISA Kit for Dog IL-10

Cat.:

E0056h

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96T

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ELISA Kit for Dog IL-10

Cat.:

E0056c

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96T

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ELISA Kit for Dog IL-10

Cat.:

E0056d

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96T

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ELISA Kit for Dog IL-10

Cat.:

E0056b

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96T

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CLIA Kit for Dog IL-10

Cat.:

U0056b

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Packing:

96T

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CLIA Kit for Dog IL-10

Cat.:

U0056p

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Manual:

Looking for manual.

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Packing:

96T

Range:

Please sign in first.

CLIA Kit for Dog IL-10

Cat.:

U0056d

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Looking for manual.

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Packing:

96T

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CLIA Kit for Dog IL-10

Cat.:

U0056c

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Manual:

Looking for manual.

MSDS:

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Packing:

96T

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CLIA Kit for Dog IL-10

Cat.:

U0056h

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Looking for manual.

MSDS:

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Packing:

96T

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CLIA Kit for Dog IL-10

Cat.:

U0056m

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Looking for manual.

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Packing:

96T

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CLIA Kit for Dog IL-10

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CLIA Kit for Dog IL-10

Cat.:

U0056r

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Polyclonal Antibody for Dog IL-10

Cat.:

P0056Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Dog IL-10

Cat.:

P0056Rb-r

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Packing:

40ug/0.2ml

Polyclonal Antibody for Dog IL-10

Cat.:

P0056Rb-m

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Packing:

40ug/0.2ml

Monoclonal Antibody for Dog IL-10

Protein for Dog IL-10

Cat.:

R0056h

Price:

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Packing:

1mg

Purity:

>90%

Protein for Dog IL-10

Cat.:

R0056m

Price:

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Packing:

1mg

Purity:

>90%

Protein for Dog IL-10

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

12% SDS-PAGE Analysis

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.4%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.3%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.0%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.2%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of IL10 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	102	99-105
Plasma	103	102-108

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of IL10 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	94-103
Plasma	100	98-105

Recovery was determined by spiking various levels of IL10 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	101	99-105
Plasma	102	100-104

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL10 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	100-105%	98-103%	107-111%	90-98%
EDTA plasma(n=5)	99-106%	103-105%	97-103%	101-104%
heparin plasma(n=5)	102-104%	90-98%	105-110%	87-95%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	94-100%	97-102%	95-98%	101-105%
EDTA plasma(n=5)	98-104%	94-103%	102-108%	89-97%
heparin plasma(n=5)	85-96%	105-109%	98-103%	97-105%

Sample	1：2	1:4	1:8	1:16
serum(n=5)	100-104%	91-98%	99-106%	103-108%
EDTA plasma(n=5)	101-104%	98-103%	105-110%	93-100%
heparin plasma(n=5)	96-103%	89-97%	101-108%	104-107%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Cloning and expression of canine interleukin-10."
Lu P. , Zucker K. , Fuller L. , Tzakis A. , Esquenazi V. , Miller J.
J. Interferon Cytokine Res.15:1103-1109(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
strain: Beagle.

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