IL10 ELISA Kit for Human(Homo sapiens) | EIAab.comIL10 ELISA Kit for Human(Homo sapiens)

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Index > Protein center > IL10(Gene name) > Human

IL10 (Gene name),

Interleukin-10 (Protein name ), IL10_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

IL10;

Protein name:

Interleukin-10(IL-10);

Alternative:

Cytokine synthesis inhibitory factor(CSIF);

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Homodimer.

Function:

Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

Subcellular Location:

Secreted

Protein Attributes:

Sequence length:

178

Sequence:

50:

100:

150:

178:

EKGIYKAMSE | FDIFINYIEA | YMTMKIRN

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

Pfam:

PF00726

STRING:

9606.ENSP00000412237

KEGG:

hsa:3586

UniGene:

Hs.193717

SMR:

P22301

String:

P22301

MIM:

124092

Uniprot:

P22301

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ELISA Kit for Human IL-10

Cat.:

E0056r

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Packing:

96T

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ELISA Kit for Human IL-10

MSDS:

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ELISA Kit for Human IL-10

Cat.:

E0056p

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96T

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ELISA Kit for Human IL-10

Cat.:

E0056m

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Packing:

96T

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ELISA Kit for Human IL-10

Cat.:

E0056h

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96T

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ELISA Kit for Human IL-10

Cat.:

E0056c

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96T

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ELISA Kit for Human IL-10

Cat.:

E0056d

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96T

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ELISA Kit for Human IL-10

Cat.:

E0056b

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96T

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CLIA Kit for Human IL-10

Cat.:

U0056b

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Packing:

96T

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CLIA Kit for Human IL-10

Cat.:

U0056p

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Looking for manual.

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Packing:

96T

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Please sign in first.

CLIA Kit for Human IL-10

Cat.:

U0056d

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Looking for manual.

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Packing:

96T

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CLIA Kit for Human IL-10

Cat.:

U0056c

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Looking for manual.

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Packing:

96T

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CLIA Kit for Human IL-10

Cat.:

U0056h

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Looking for manual.

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Packing:

96T

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CLIA Kit for Human IL-10

Cat.:

U0056m

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Looking for manual.

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Packing:

96T

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CLIA Kit for Human IL-10

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CLIA Kit for Human IL-10

Cat.:

U0056r

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96T

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Polyclonal Antibody for Human IL-10

Cat.:

P0056Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human IL-10

Cat.:

P0056Rb-r

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human IL-10

Cat.:

P0056Rb-m

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Packing:

40ug/0.2ml

Monoclonal Antibody for Human IL-10

Protein for Human IL-10

Cat.:

R0056h

Price:

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Packing:

1mg

Purity:

>90%

Protein for Human IL-10

Cat.:

R0056m

Price:

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Packing:

1mg

Purity:

>90%

Protein for Human IL-10

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

12% SDS-PAGE Analysis

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.4%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.3%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.0%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.2%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of IL10 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	102	99-105
Plasma	103	102-108

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of IL10 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	94-103
Plasma	100	98-105

Recovery was determined by spiking various levels of IL10 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	101	99-105
Plasma	102	100-104

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL10 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	100-105%	98-103%	107-111%	90-98%
EDTA plasma(n=5)	99-106%	103-105%	97-103%	101-104%
heparin plasma(n=5)	102-104%	90-98%	105-110%	87-95%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	94-100%	97-102%	95-98%	101-105%
EDTA plasma(n=5)	98-104%	94-103%	102-108%	89-97%
heparin plasma(n=5)	85-96%	105-109%	98-103%	97-105%

Sample	1：2	1:4	1:8	1:16
serum(n=5)	100-104%	91-98%	99-106%	103-108%
EDTA plasma(n=5)	101-104%	98-103%	105-110%	93-100%
heparin plasma(n=5)	96-103%	89-97%	101-108%	104-107%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Isolation and expression of human cytokine synthesis inhibitory factor cDNA clones: homology to Epstein-Barr virus open reading frame BCRFI."
Vieira P. , de Waal-Malefyt R. , Dang M.-N. , Johnson K.E. , Kastelein R. , Fiorentino D.F. , Devries J.E. , Roncarolo M.-G. , Mosmann T.R. , Moore K.W.
Proc. Natl. Acad. Sci. U.S.A.88:1172-1176(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
tissue: T-cell.
2.

"The structure of the human IL-10 gene."
Sanjanwala B. , de Waal-Malefyt R.
Submitted (1994-10) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
3.

"Cloning, sequencing of human interleukin-10 cDNA and construction of its eukaryotic expression vector."
Dai W.-J. , Jiang H.-C. , Pan S.-H.
Haerbin Yi Ke Da Xue Xue Bao35:4-6(2001)

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
4.

SeattleSNPs variation discovery resource
Submitted (2001-09) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
5.

"Identification of functional domains on human interleukin 10."
Gesser B. , Leffers H. , Jinquan T. , Vestergaard C. , Kirstein N. , Sindet-Pedersen S. , Jensen S.L. , Thestrup-Pedersen K. , Larsen C.G.
Proc. Natl. Acad. Sci. U.S.A.94:14620-14625(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 26-34 AND 170-178
6.

"Signal peptide prediction based on analysis of experimentally verified cleavage sites."
Zhang Z. , Henzel W.J.
Protein Sci.13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 19-33
7.

"Disulfide bond assignments and secondary structure analysis of human and murine interleukin 10."
Windsor W.T. , Syto R. , Tsarbopoulos A. , Zhang R. , Durkin J. , Baldwin S. , Paliwal S. , Mui P.W. , Pramanik B. , Trotta P.P.
Biochemistry32:8807-8815(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISULFIDE BONDS
8.

"Relation of an interleukin-10 promoter polymorphism to graft-versus-host disease and survival after hematopoietic-cell transplantation."
Lin M.T. , Storer B. , Martin P.J. , Tseng L.H. , Gooley T. , Chen P.J. , Hansen J.A.
N. Engl. J. Med.349:2201-2210(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INVOLVEMENT IN RESISTANCE TO GVHDS
9.

"Crystal structure of interleukin 10 reveals an interferon gamma-like fold."
Walter M.R. , Nagabhushan T.L.
Biochemistry34:12118-12125(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS)
10.

"Crystal structure of interleukin-10 reveals the functional dimer with an unexpected topological similarity to interferon gamma."
Zdanov A. , Schalk-Hihi C. , Gustchina A. , Tsang M. , Wheatherbee J. , Wlodawer A.
Structure3:591-601(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS)
11.

"Crystal structure of human interleukin-10 at 1.6-A resolution and a model of a complex with its soluble receptor."
Zdanov A. , Schalk-Hihi C. , Wlodawer A.
Protein Sci.5:1955-1962(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS)
12.

"Crystal structure of the IL-10/IL-10R1 complex reveals a shared receptor binding site."
Josephson K. , Logsdon N.J. , Walter M.R.
Immunity15:35-46(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 19-178 IN COMPLEX WITH IL10R1
13.

"Same structure, different function crystal structure of the Epstein-Barr virus IL-10 bound to the soluble IL-10R1 chain."
Yoon S.I. , Jones B.C. , Logsdon N.J. , Walter M.R.
Structure13:551-564(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 19-178 IN COMPLEX WITH IL10R1
14.

"A Gly15Arg mutation in the interleukin-10 gene reduces secretion of interleukin-10 in Crohn disease."
van der Linde K. , Boor P.P. , Sandkuijl L.A. , Meijssen M.A. , Savelkoul H.F. , Wilson J.H. , de Rooij F.W.
Scand. J. Gastroenterol.38:611-617(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT ARG-15

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