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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.7%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.1%
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Recovery
Recovery was determined by spiking various levels of IL-18 into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
102
96-106
Plasma
101
99-104
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Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-18 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
100-105%
94-101%
95-102%
92-97%
EDTA plasma(n=5)
90-95%
94-106%
105-107%
87-98%
heparin plasma(n=5)
95-103%
91-99%
104-107%
98-105%
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[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2);PROTEOLYTIC PROCESSING;ALTERNATIVE SPLICING
3.
"Cloning and sequencing of the cDNA for precursor hIL-18." Yong D.
,
Guixin D.
,
Lihua H.
,
Haitao W.
Submitted (1998-07) to the EMBL/GenBank/DDBJ databases
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
4.
"Cloning of human interleukin 18 cDNA." Liu J.
,
Peng X.
,
Yuan J.
,
Qiang B.
Submitted (2001-07) to the EMBL/GenBank/DDBJ databases
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
5.
"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L.
,
Schick M.
,
Neubert P.
,
Schatten R.
,
Henze S.
,
Korn B.
Submitted (2004-06) to the EMBL/GenBank/DDBJ databases
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-193 (ISOFORM 1)
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]