Leptin (Protein name
), LEP_RAT from NCBI database.
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General Annotation
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Antigen Annotation
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3D
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Predicted Eptitope
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Vaild Sequence
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Related Databases
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Citations (1)
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Feedback
Gene name:
Lep(Ob);
Protein name:
Leptin;
Alternative:
Obesity factor;
Organism:
Rat (Rattus norvegicus).
General Annotation
Sub Unit:
N/A
Function:
May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of Lep may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
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Precision
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤3.5%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.2%
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤3.9%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.9%
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Recovery
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Recovery was determined by spiking various levels of LEP into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
89
84-90
Plasma
88
86-94
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Recovery was determined by spiking various levels of LEP into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
101
97-105
Plasma
102
99-103
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Linearity
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LEP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1：2
1:4
1:8
1:16
serum(n=5)
81-88%
85-93%
90-95%
80-84%
EDTA plasma(n=5)
85-89%
92-96%
83-87%
86-93%
heparin plasma(n=5)
88-94%
80-86%
90-97%
87-95%
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LEP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1：2
1:4
1:8
1:16
serum(n=5)
102-106%
97-103%
91-98%
103-107%
EDTA plasma(n=5)
88-95%
95-105%
104-109%
95-103%
heparin plasma(n=5)
99-108%
95-102%
100-106%
96-104%
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Vaild Sequence:
