MED24 ELISA Kit for Human(Homo sapiens) | EIAab.comMED24 ELISA Kit for Human(Homo sapiens) | EIAab.com

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MED24 (Gene name),

Mediator of RNA polymerase II transcription subunit 24 (Protein name ), MED24_HUMAN from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Related Databases

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Gene name:

MED24(ARC100;CRSP4;DRIP100;KIAA0130;THRAP4;TRAP100);

Protein name:

Mediator of RNA polymerase II transcription subunit 24;

Alternative:

Cofactor required for Sp1 transcriptional activation subunit 4(CRSP complex subunit 4);Activator-recruited cofactor 100 kDa component(ARC100);Thyroid hormone receptor-associated protein 4;Mediator complex subunit 24;Vitamin D3 receptor-interacting protein complex 100 kDa component(DRIP100);Thyroid hormone receptor-associated protein complex 100 kDa component(Trap100;hTRAP100);

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with AR.

Function:

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Subcellular Location:

Nucleus

Protein Attributes:

Sequence length:

989

Sequence:

50:

MKVVNLKQAI | LQAWKERWSD | YQWAINMKKF | FPKGATWDIL | NLADALLEQA |

100:

MIGPSPNPLI | LSYLKYAISS | QMVSYSSVLT | AISKFDDFSR | DLCVQALLDI |

150:

MDMFCDRLSC | HGKAEECIGL | CRALLSALHW | LLRCTAASAE | RLREGLEAGT |

200:

PAAGEKQLAM | CLQRLEKTLS | STKNRALLHI | AKLEEASSWT | AIEHSLLKLG |

250:

EILANLSNPQ | LRSQAEQCGT | LIRSIPTMLS | VHAEQMHKTG | FPTVHAVILL |

300:

EGTMNLTGET | QSLVEQLTMV | KRMQHIPTPL | FVLEIWKACF | VGLIESPEGT |

350:

EELKWTAFTF | LKIPQVLVKL | KKYSHGDKDF | TEDVNCAFEF | LLKLTPLLDK |

400:

ADQRCNCDCT | NFLLQECGKQ | GLLSEASVNN | LMAKRKADRE | HAPQQKSGEN |

450:

ANIQPNIQLI | LRAEPTVTNI | LKTMDADHSK | SPEGLLGVLG | HMLSGKSLDL |

500:

LLAAAAATGK | LKSFARKFIN | LNEFTTYGSE | ESTKPASVRA | LLFDISFLML |

550:

CHVAQTYGSE | VILSESRTGA | EVPFFETWMQ | TCMPEEGKIL | NPDHPCFRPD |

600:

STKVESLVAL | LNNSSEMKLV | QMKWHEACLS | ISAAILEILN | AWENGVLAFE |

650:

SIQKITDNIK | GKVCSLAVCA | VAWLVAHVRM | LGLDEREKSL | QMIRQLAGPL |

700:

FSENTLQFYN | ERVVIMNSIL | ERMCADVLQQ | TATQIKFPST | GVDTMPYWNL |

750:

LPPKRPIKEV | LTDIFAKVLE | KGWVDSRSIH | IFDTLLHMGG | VYWFCNNLIK |

800:

ELLKETRKEH | TLRAVELLYS | IFCLDMQQVT | LVLLGHILPG | LLTDSSKWHS |

850:

LMDPPGTALA | KLAVWCALSS | YSSHKGQAST | RQKKRHREDI | EDYISLFPLD |

900:

DVQPSKLMRL | LSSNEDDANI | LSSPTDRSMS | SSLSASQLHT | VNMRDPLNRV |

950:

LANLFLLISS | ILGSRTAGPH | TQFVQWFMEE | CVDCLEQGGR | GSVLQFMPFT |

989:

TVSELVKVSA | MSSPKVVLAI | TDLSLPLGRQ | VAAKAIAAL

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

KEGG:

String:

MIM:

UniGene:

STRING:

9606.ENSP00000348610

Pfam:

Uniprot:

FOR

ELISA Kit for Human Mediator of RNA polymerase II transcription subunit 24

ELISA Kit for Human Mediator of RNA polymerase II transcription subunit 24

ELISA Kit for Human Mediator of RNA polymerase II transcription subunit 24

ELISA Kit for Human Mediator of RNA polymerase II transcription subunit 24

CLIA Kit for Human Mediator of RNA polymerase II transcription subunit 24

CLIA Kit for Human Mediator of RNA polymerase II transcription subunit 24

CLIA Kit for Human Mediator of RNA polymerase II transcription subunit 24

CLIA Kit for Human Mediator of RNA polymerase II transcription subunit 24

Polyclonal Antibody for Human Mediator of RNA polymerase II transcription subunit 24

Polyclonal Antibody for Human Mediator of RNA polymerase II transcription subunit 24

Polyclonal Antibody for Human Mediator of RNA polymerase II transcription subunit 24

Polyclonal Antibody for Human Mediator of RNA polymerase II transcription subunit 24

Monoclonal Antibody for Human Mediator of RNA polymerase II transcription subunit 24

Monoclonal Antibody for Human Mediator of RNA polymerase II transcription subunit 24

Monoclonal Antibody for Human Mediator of RNA polymerase II transcription subunit 24

Monoclonal Antibody for Human Mediator of RNA polymerase II transcription subunit 24

Protein for Human Mediator of RNA polymerase II transcription subunit 24

Protein for Human Mediator of RNA polymerase II transcription subunit 24

Protein for Human Mediator of RNA polymerase II transcription subunit 24

Protein for Human Mediator of RNA polymerase II transcription subunit 24

R&D Technical Data

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Precision

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Recovery

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Linearity

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References

1.

"The TRAP220 component of a thyroid hormone receptor-associated protein (TRAP) coactivator complex interacts directly with nuclear receptors in a ligand-dependent fashion."
Yuan C.-X. , Ito M. , Fondell J.D. , Fu Z.-Y. , Roeder R.G.
Proc. Natl. Acad. Sci. U.S.A.95:7939-7944(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);PROTEIN SEQUENCE OF 1-15;TISSUE SPECIFICITY
2.

"A novel protein complex that interacts with the vitamin D3 receptor in a ligand-dependent manner and enhances VDR transactivation in a cell-free system."
Rachez C. , Suldan Z. , Ward J. , Chang C.-P.B. , Burakov D. , Erdjument-Bromage H. , Tempst P. , Freedman L.P.
Genes Dev.12:1787-1800(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);IDENTIFICATION IN DRIP COMPLEX
3.

"Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1."
Nagase T. , Seki N. , Tanaka A. , Ishikawa K. , Nomura N.
DNA Res.2:167-174(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1);VARIANT THR-204
tissue: Bone marrow.
4.

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2)
tissue: Teratocarcinoma.
5.

"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
Zody M.C. , Garber M. , Adams D.J. , Sharpe T. , Harrow J. , Lupski J.R. , Nicholson C. , Searle S.M. , Wilming L. , Young S.K. , Abouelleil A. , Allen N.R. , Bi W. , Bloom T. , Borowsky M.L. , Bugalter B.E. , Butler J. , Chang J.L. , more...
Nature440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
6.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
tissue: Eye.
7.

"Composite co-activator ARC mediates chromatin-directed transcriptional activation."
Naeaer A.M. , Beaurang P.A. , Zhou S. , Abraham S. , Solomon W.B. , Tjian R.
Nature398:828-832(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 1-11 AND 957-965;IDENTIFICATION IN ARC COMPLEX
8.

"Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex."
Rachez C. , Lemon B.D. , Suldan Z. , Bromleigh V. , Gamble M. , Naeaer A.M. , Erdjument-Bromage H. , Tempst P. , Freedman L.P.
Nature398:824-828(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 467-489;IDENTIFICATION IN ARC COMPLEX
tissue: Cervix carcinoma.
9.

"A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation."
Gu W. , Malik S. , Ito M. , Yuan C.-X. , Fondell J.D. , Zhang X. , Martinez E. , Qin J. , Roeder R.G.
Mol. Cell3:97-108(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY;IDENTIFICATION IN THE SMCC COMPLEX
10.

Gu W. , Malik S. , Ito M. , Yuan C.-X. , Fondell J.D. , Zhang X. , Martinez E. , Qin J. , Roeder R.G.
Mol. Cell3:541-541(1999)

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ERRATUM
11.

"A coregulatory role for the TRAP-mediator complex in androgen receptor-mediated gene expression."
Wang Q. , Sharma D. , Ren Y. , Fondell J.D.
J. Biol. Chem.277:42852-42858(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH AR
12.

"A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
Sato S. , Tomomori-Sato C. , Parmely T.J. , Florens L. , Zybailov B. , Swanson S.K. , Banks C.A.S. , Jin J. , Cai Y. , Washburn M.P. , Conaway J.W. , Conaway R.C.
Mol. Cell14:685-691(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY;IDENTIFICATION IN THE MEDIATOR COMPLEX
13.

"MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."
Zhang X. , Krutchinsky A. , Fukuda A. , Chen W. , Yamamura S. , Chait B.T. , Roeder R.G.
Mol. Cell19:89-100(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH MED1; MED10; MED21 AND MED30;IDENTIFICATION BY MASS SPECTROMETRY;IDENTIFICATION IN THE MEDIATOR COMPLEX;ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II
14.

"Human Mediator enhances basal transcription by facilitating recruitment of transcription factor IIB during preinitiation complex assembly."
Baek H.J. , Kang Y.K. , Roeder R.G.
J. Biol. Chem.281:15172-15181(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH MED1 AND MED10
15.

"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H. , Olsen J.V. , Bairlein M. , Gnad F. , Oppermann F.S. , Korner R. , Greff Z. , Keri G. , Stemmann O. , Mann M.
Mol. Cell31:438-448(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
16.

"A quantitative atlas of mitotic phosphorylation."
Dephoure N. , Zhou C. , Villen J. , Beausoleil S.A. , Bakalarski C.E. , Elledge S.J. , Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A.105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-862 AND SER-873;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
17.

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S. , Helbig A.O. , Slijper M. , Krijgsveld J. , Heck A.J. , Mohammed S.
Anal. Chem.81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
18.

"Large-scale proteomics analysis of the human kinome."
Oppermann F.S. , Gnad F. , Olsen J.V. , Hornberger R. , Greff Z. , Keri G. , Mann M. , Daub H.
Mol. Cell. Proteomics8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
19.

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V. , Lundgren D.H. , Hwang S.-I. , Rezaul K. , Wu L. , Eng J.K. , Rodionov V. , Han D.K.
Sci. Signal.2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-862 AND SER-873;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Leukemic T-cell.
20.

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V. , Vermeulen M. , Santamaria A. , Kumar C. , Miller M.L. , Jensen L.J. , Gnad F. , Cox J. , Jensen T.S. , Nigg E.A. , Brunak S. , Mann M.
Sci. Signal.3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-862 AND SER-873;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
21.

"Initial characterization of the human central proteome."
Burkard T.R. , Planyavsky M. , Kaupe I. , Breitwieser F.P. , Buerckstuemmer T. , Bennett K.L. , Superti-Furga G. , Colinge J.
BMC Syst. Biol.5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
22.

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T. , Prokhorova T.A. , Akimov V. , Henningsen J. , Johansen P.T. , Kratchmarova I. , Kassem M. , Mann M. , Olsen J.V. , Blagoev B.
Sci. Signal.4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-862;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]

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