NGF ELISA Kit for Human(Homo sapiens) | EIAab.comNGF ELISA Kit for Human(Homo sapiens)

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Index > Protein center > NGF(Gene name) > Human

NGF (Gene name),

Beta-nerve growth factor (Protein name ), NGF_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

Citations (2)

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Gene name:

NGF(NGFB);

Protein name:

Beta-nerve growth factor(Beta-NGF);

Alternative:

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Homodimer.

Function:

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons.

Subcellular Location:

Secreted

Protein Attributes:

Sequence length:

241

Sequence:

50:

100:

150:

200:

241:

CTTTHTFVKA | LTMDGKQAAW | RFIRIDTACV | CVLSRKAVRR | A

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

STRING:

9606.ENSP00000358525

KEGG:

hsa:4803

Pfam:

PF00243

String:

P01138

UniGene:

Hs.2561

SMR:

P01138

MIM:

162030

Uniprot:

P01138

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ELISA Kit for Human Beta-NGF

Cat.:

E0105r

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96T

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ELISA Kit for Human Beta-NGF

Cat.:

E0105h

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96T

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ELISA Kit for Human Beta-NGF

Cat.:

E0105p

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96T

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ELISA Kit for Human Beta-NGF

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E0105m

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96T

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ELISA Kit for Human Beta-NGF

Cat.:

E0105c

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96T

ELISA Kit for Human Beta-NGF

Cat.:

E0105b

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96T

CLIA Kit for Human Beta-NGF

Cat.:

U0105c

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96T

CLIA Kit for Human Beta-NGF

Cat.:

U0105b

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Packing:

96T

CLIA Kit for Human Beta-NGF

Cat.:

U0105h

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Please sign in first.

Packing:

96T

CLIA Kit for Human Beta-NGF

Cat.:

U0105m

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Please sign in first.

Packing:

96T

CLIA Kit for Human Beta-NGF

Cat.:

U0105p

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Manual:

Looking for manual.

MSDS:

Please sign in first.

Packing:

96T

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Please sign in first.

CLIA Kit for Human Beta-NGF

Cat.:

U0105r

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Looking for manual.

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96T

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Polyclonal Antibody for Human Beta-NGF

Cat.:

P0105Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human Beta-NGF

Cat.:

P0105Rb-m

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40ug/0.2ml

Polyclonal Antibody for Human Beta-NGF

Cat.:

P0105Rb-r

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human Beta-NGF

Monoclonal Antibody for Human Beta-NGF

Protein for Human Beta-NGF

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.0%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.3%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.3%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of NGF into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	91-100
Plasma	98	95-106

Recovery was determined by spiking various levels of NGF into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	100	94-105
Plasma	101	98-107

Recovery was determined by spiking various levels of NGF into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	92-103
Plasma	98	97-105

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NGF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	89-97%	92-102%	100-104%	91-98%
EDTA plasma(n=5)	90-98%	95-100%	90-96%	99-104%
heparin plasma(n=5)	95-102%	93-104%	97-102%	85-96%

Sample	1：2	1:4	1:8	1:16
serum(n=5)	100-106%	94-103%	90-102%	97-105%
EDTA plasma(n=5)	87-94%	91-99%	102-107%	103-110%
heparin plasma(n=5)	102-106%	91-96%	97-104%	95-106%

Sample	1：2	1:4	1:8	1:16
serum(n=5)	90-100%	97-104%	93-105%	102-107%
EDTA plasma(n=5)	97-101%	102-105%	89-94%	94-103%
heparin plasma(n=5)	102-106%	90-97%	97-103%	92-105%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Citations

[1].

"Production of functional human nerve growth factor from the saliva of transgenic mice by using salivary glands as bioreactors"

Fang Z, Zicong L, Qingchun Z, et al.

From:Scientific Reports

[2].

"Urinary biomarkers in patients with detrusor underactivity with and without bladder function recovery"

Sheng-Fu C, Yuan-Hong J, Hann-Chorng K.

From:International Urology and Nephrology

References

1.

"Human beta-nerve growth factor gene sequence highly homologous to that of mouse."
Ullrich A. , Gray A. , Berman C. , Dull T.J.
Nature303:821-825(1983) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANT VAL-35
2.

"Sequence homology of human and mouse beta-NGF subunit genes."
Ullrich A. , Gray A. , Berman C. , Coussens L. , Dull T.J.
Cold Spring Harb. Symp. Quant. Biol.48:435-442(1983) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANT VAL-35
3.

"cDNA sequence of human beta-NGF."
Borsani G. , Pizzuti A. , Rugarli E.I. , Falini A. , Silani V. , Sidoli A. , Scarlato G. , Barelle F.E.
Nucleic Acids Res.18:4020-4020(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];VARIANT VAL-35
tissue: Brain.
4.

"Cloning and sequencing of the gene for premature beta nerve growth factor."
Tong Y. , Wang H. , Chen W.
Zhongguo Ying Yong Sheng Li Xue Za Zhi13:316-318(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
5.

"Human-specific amino acid changes found in 103 protein-coding genes."
Kitano T. , Liu Y.-H. , Ueda S. , Saitou N.
Mol. Biol. Evol.21:936-944(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANT VAL-35
6.

Zhang Y. , Zhang B. , Zhou Y. , Peng X. , Yuan J. , Qiang B.
Submitted (2001-08) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
7.

"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Halleck A. , Ebert L. , Mkoundinya M. , Schick M. , Eisenstein S. , Neubert P. , Kstrang K. , Schatten R. , Shen B. , Henze S. , Mar W. , Korn B. , Zuo D. , Hu Y. , LaBaer J.
Submitted (2004-06) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
8.

"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N. , Chen X. , Rolfs A. , Halleck A. , Hines L. , Eisenstein S. , Koundinya M. , Raphael J. , Moreira D. , Kelley T. , LaBaer J. , Lin Y. , Phelan M. , Farmer A.
Submitted (2004-10) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
9.

"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G. , Barlow K.F. , McLay K.E. , Kaul R. , Swarbreck D. , Dunham A. , Scott C.E. , Howe K.L. , Woodfine K. , Spencer C.C.A. , Jones M.C. , Gillson C. , Searle S. , Zhou Y. , Kokocinski F. , McDonald L. , Evans R. , Phillips K. , more...
Nature441:315-321(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
10.

Mural R.J. , Istrail S. , Sutton G.G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA];VARIANT VAL-35
11.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA];VARIANT VAL-35
tissue: Eye.
12.

"Evolutionary studies of the nerve growth factor family reveal a novel member abundantly expressed in Xenopus ovary."
Hallboeoek F. , Ibanez C.F. , Persson H.
Neuron6:845-858(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 178-219
tissue: Leukocyte.
13.

"The trk proto-oncogene encodes a receptor for nerve growth factor."
Klein R. , Jing S. , Nanduri V. , O'Rourke E. , Barbacid M.
Cell65:189-197(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION OF NTRK1 AS THE HIGH AFFINITY NGF RECEPTOR
14.

"Crystal structure of nerve growth factor in complex with the ligand-binding domain of the TrkA receptor."
Wiesmann C. , Ultsch M.H. , Bass S.H. , de Vos A.M.
Nature401:184-188(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 122-241;DISULFIDE BONDS
15.

"Characterization of single-nucleotide polymorphisms in coding regions of human genes."
Cargill M. , Altshuler D. , Ireland J. , Sklar P. , Ardlie K. , Patil N. , Shaw N. , Lane C.R. , Lim E.P. , Kalyanaraman N. , Nemesh J. , Ziaugra L. , Friedland L. , Rolfe A. , Warrington J. , Lipshutz R. , Daley G.Q. , Lander E.S.
Nat. Genet.22:231-238(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT VAL-35
16.

Cargill M. , Altshuler D. , Ireland J. , Sklar P. , Ardlie K. , Patil N. , Shaw N. , Lane C.R. , Lim E.P. , Kalyanaraman N. , Nemesh J. , Ziaugra L. , Friedland L. , Rolfe A. , Warrington J. , Lipshutz R. , Daley G.Q. , Lander E.S.
Nat. Genet.23:373-373(1999)

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ERRATUM
17.

"A mutation in the nerve growth factor beta gene (NGFB) causes loss of pain perception."
Einarsdottir E. , Carlsson A. , Minde J. , Toolanen G. , Svensson O. , Solders G. , Holmgren G. , Holmberg D. , Holmberg M.
Hum. Mol. Genet.13:799-805(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT HSAN5 TRP-221
18.

"A novel NGF mutation clarifies the molecular mechanism and extends the phenotypic spectrum of the HSAN5 neuropathy."
Carvalho O.P. , Thornton G.K. , Hertecant J. , Houlden H. , Nicholas A.K. , Cox J.J. , Rielly M. , Al-Gazali L. , Woods C.G.
J. Med. Genet.48:131-135(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CHARACTERIZATION OF VARIANT HSAN5 TRP-221
19.

"Frequency of mutations in the genes associated with hereditary sensory and autonomic neuropathy in a UK cohort."
Davidson G.L. , Murphy S.M. , Polke J.M. , Laura M. , Salih M.A. , Muntoni F. , Blake J. , Brandner S. , Davies N. , Horvath R. , Price S. , Donaghy M. , Roberts M. , Foulds N. , Ramdharry G. , Soler D. , Lunn M.P. , Manji H. , more...
J. Neurol.259:1673-1685(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT HSAN5 GLY-GLU-162 INS;VARIANT ASN-187

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