Ngf ELISA Kit for Rat(Rattus norvegicus) | EIAab.comNgf ELISA Kit for Rat(Rattus norvegicus)

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Index > Protein center > Ngf(Gene name) > Rat

Ngf (Gene name),

Beta-nerve growth factor (Protein name ), NGF_RAT from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

Citations (1)

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Gene name:

Ngf(Ngfb);

Protein name:

Beta-nerve growth factor(Beta-NGF);

Alternative:

Organism:

Rat (Rattus norvegicus).

General Annotation

Sub Unit:

Homodimer.

Function:

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons.

Subcellular Location:

Secreted

Protein Attributes:

Sequence length:

241

Sequence:

50:

100:

150:

200:

241:

CTTTHTFVKA | LTTDDKQAAW | RFIRIDTACV | CVLSRKAARR | G

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

STRING:

10116.ENSRNOP00000022200

UniGene:

Rn.22168

KEGG:

rno:310738

String:

P25427

Pfam:

PF00243

SMR:

P25427

Uniprot:

P25427

FOR

ELISA Kit for Rat Beta-NGF

Cat.:

E0105r

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96T

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ELISA Kit for Rat Beta-NGF

Cat.:

E0105h

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96T

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ELISA Kit for Rat Beta-NGF

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E0105p

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ELISA Kit for Rat Beta-NGF

Cat.:

E0105m

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ELISA Kit for Rat Beta-NGF

Cat.:

E0105c

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96T

ELISA Kit for Rat Beta-NGF

Cat.:

E0105b

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96T

CLIA Kit for Rat Beta-NGF

Cat.:

U0105c

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96T

CLIA Kit for Rat Beta-NGF

Cat.:

U0105b

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Packing:

96T

CLIA Kit for Rat Beta-NGF

Cat.:

U0105h

Price:

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MSDS:

Please sign in first.

Packing:

96T

CLIA Kit for Rat Beta-NGF

Cat.:

U0105m

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MSDS:

Please sign in first.

Packing:

96T

CLIA Kit for Rat Beta-NGF

Cat.:

U0105p

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Manual:

Looking for manual.

MSDS:

Please sign in first.

Packing:

96T

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Please sign in first.

CLIA Kit for Rat Beta-NGF

Cat.:

U0105r

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Looking for manual.

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96T

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Polyclonal Antibody for Rat Beta-NGF

Cat.:

P0105Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Rat Beta-NGF

Cat.:

P0105Rb-m

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40ug/0.2ml

Polyclonal Antibody for Rat Beta-NGF

Cat.:

P0105Rb-r

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Packing:

40ug/0.2ml

Polyclonal Antibody for Rat Beta-NGF

Monoclonal Antibody for Rat Beta-NGF

Protein for Rat Beta-NGF

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.0%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.3%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.3%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of NGF into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	91-100
Plasma	98	95-106

Recovery was determined by spiking various levels of NGF into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	100	94-105
Plasma	101	98-107

Recovery was determined by spiking various levels of NGF into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	92-103
Plasma	98	97-105

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NGF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	89-97%	92-102%	100-104%	91-98%
EDTA plasma(n=5)	90-98%	95-100%	90-96%	99-104%
heparin plasma(n=5)	95-102%	93-104%	97-102%	85-96%

Sample	1：2	1:4	1:8	1:16
serum(n=5)	100-106%	94-103%	90-102%	97-105%
EDTA plasma(n=5)	87-94%	91-99%	102-107%	103-110%
heparin plasma(n=5)	102-106%	91-96%	97-104%	95-106%

Sample	1：2	1:4	1:8	1:16
serum(n=5)	90-100%	97-104%	93-105%	102-107%
EDTA plasma(n=5)	97-101%	102-105%	89-94%	94-103%
heparin plasma(n=5)	102-106%	90-97%	97-103%	92-105%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Citations

[1].

"Telmisartan inhibits hyperalgesia and inflammatory progression in a diabetic neuropathic pain model of Wistar rats"

SS Al-Rejaie, HM Abuohashish, MM Ahmed, et al.

From:Neurosciences (Riyadh, Saudi Arabia)

References

1.

"Rat beta-nerve growth factor sequence and site of synthesis in the adult hippocampus."
Whittemore S.R. , Friedman P.L. , Larhammar D.G. , Persson H. , Gonzalez-Carvajal M. , Holets V.R.
J. Neurosci. Res.20:403-410(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
2.

"Evolutionary studies of the nerve growth factor family reveal a novel member abundantly expressed in Xenopus ovary."
Hallboeoek F. , Ibanez C.F. , Persson H.
Neuron6:845-858(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 178-219
strain: Sprague-Dawley.
tissue: Liver.
3.

"A novel NGF mutation clarifies the molecular mechanism and extends the phenotypic spectrum of the HSAN5 neuropathy."
Carvalho O.P. , Thornton G.K. , Hertecant J. , Houlden H. , Nicholas A.K. , Cox J.J. , Rielly M. , Al-Gazali L. , Woods C.G.
J. Med. Genet.48:131-135(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MUTAGENESIS OF ARG-221

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