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Index > Protein center > Nos2(Gene name) > Rat
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  • Nos2 (Gene name),
  • Nitric oxide synthase, inducible (Protein name ),  NOS2_RAT from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Gene name:
    Nos2;
    Protein name:
    Nitric oxide synthase, inducible;
    Alternative:
    NOS type II;Inducible NO synthase(Inducible NOS;iNOS);Peptidyl-cysteine S-nitrosylase NOS2;
    Organism:
    Rat (Rattus norvegicus). 
    General Annotation
    Sub Unit:
    Homodimer. Binds SLC9A3R1.
    Function:
    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.
    Subcellular Location:
    N/A
    Protein Attributes:
    Sequence length:
    1147
    Sequence:
    50:
    MACPWKFLFR | VKSYQGDLKE | EKDINNNVEK | TPGAIPSPTT | QDDPKSHKHQ | 
    100:
    NGFPQFLTGT | AQNVPESLDK | LHVTPSTRPQ | HVRIKNWGNG | EIFHDTLHHK | 
    150:
    ATSDISCKSK | LCMGSIMNSK | SLTRGPRDKP | TPVEELLPQA | IEFINQYYGS | 
    200:
    FKEAKIEEHL | ARLEAVTKEI | ETTGTYQLTL | DELIFATKMA | WRNAPRCIGR | 
    250:
    IQWSNLQVFD | ARSCSTASEM | FQHICRHILY | ATNSGNIRSA | ITVFPQRSDG | 
    300:
    KHDFRIWNSQ | LIRYAGYQMP | DGTIRGDPAT | LEFTQLCIDL | GWKPRYGRFD | 
    350:
    VLPLVLQAHG | QDPEVFEIPP | DLVLEVTMEH | PKYEWFQELG | LKWYALPAVA | 
    400:
    NMLLEVGGLE | FPACPFNGWY | MGTEIGVRDF | CDTQRYNILE | EVGRRMGLET | 
    450:
    HTLASLWKDR | AVTEINAAVL | HSFQKQNVTI | MDHHTASESF | MKHMQNEYRA | 
    500:
    RGGCPADWIW | LVPPVSGSIT | PVFHQEMLNY | VLSPFYYYQI | EPWKTHIWQD | 
    550:
    EKLRPRRREI | RFTVLVKAVF | FASVLMRKVM | ASRVRATVLF | ATETGKSEAL | 
    600:
    ARDLAALFSY | AFNTKVVCME | QYKANTLEEE | QLLLVVTSTF | GNGDCPSNGQ | 
    650:
    TLKKSLFMMK | ELGHTFRYAV | FGLGSSMYPQ | FCAFAHDIDQ | KLSHLGASQL | 
    700:
    APTGEGDELS | GQEDAFRSWA | VQTFRAACET | FDVRSKHCIQ | IPKRYTSNAT | 
    750:
    WEPEQYKLTQ | SPESLDLNKA | LSSIHAKNVF | TMRLKSLQNL | QSEKSSRTTL | 
    800:
    LVQLTFEGSR | GPSYLPGEHL | GIFPGNQTAL | VQGILERVVD | CSSPDQTVCL | 
    850:
    EVLDESGSYW | VKDKRLPPCS | LRQALTYFLD | ITTPPTQLQL | HKLARFATEE | 
    900:
    THRQRLEALC | QPSEYNDWKF | SNNPTFLEVL | EEFPSLRVPA | AFLLSQLPIL | 
    950:
    KPRYYSISSS | QDHTPSEVHL | TVAVVTYRTR | DGQGPLHHGV | CSTWINNLKP | 
    1000:
    EDPVPCFVRS | VSGFQLPEDP | SQPCILIGPG | TGIAPFRSFW | QQRLHDSQHR | 
    1050:
    GLKGGRMTLV | FGCRHPEEDH | LYQEEMQEMV | RKGVLFQVHT | GYSRLPGKPK | 
    1100:
    VYVQDILQKE | LADEVFSVLH | GEQGHLYVCG | DVRMARDVAT | TLKKLVAAKL | 
    1147:
    NLSEEQVEDY | FFQLKSQKRY | HEDIFGAVFS | YGAKKGNTLE | EPKGTRL
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
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    Related Databases
    String:
    UniGene:
    Pfam:
    SMR:
    KEGG:
    Uniprot:
     
    FOR
    ELISA Kit for Rat Nitric oxide synthase, inducible
    MSDS:
    Please sign in first.
    ELISA Kit for Rat Nitric oxide synthase, inducible
    Cat.:
    E0837r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Rat Nitric oxide synthase, inducible
    Cat.:
    E0837m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Rat Nitric oxide synthase, inducible
    Cat.:
    E0837h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Rat Nitric oxide synthase, inducible
    Cat.:
    E0837b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Rat Nitric oxide synthase, inducible
    Cat.:
    E0837p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Rat Nitric oxide synthase, inducible
    Cat.:
    E0837d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Rat Nitric oxide synthase, inducible
    Cat.:
    E0837c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat Nitric oxide synthase, inducible
    Cat.:
    U0837m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat Nitric oxide synthase, inducible
    Cat.:
    U0837d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat Nitric oxide synthase, inducible
    MSDS:
    Please sign in first.
    CLIA Kit for Rat Nitric oxide synthase, inducible
    MSDS:
    Please sign in first.
    CLIA Kit for Rat Nitric oxide synthase, inducible
    Cat.:
    U0837b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat Nitric oxide synthase, inducible
    Cat.:
    U0837c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat Nitric oxide synthase, inducible
    Cat.:
    U0837r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat Nitric oxide synthase, inducible
    Cat.:
    U0837h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Rat Nitric oxide synthase, inducible
    Cat.:
    P0837Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat Nitric oxide synthase, inducible
    Polyclonal Antibody for Rat Nitric oxide synthase, inducible
    Polyclonal Antibody for Rat Nitric oxide synthase, inducible
    Cat.:
    P0837Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat Nitric oxide synthase, inducible
    Polyclonal Antibody for Rat Nitric oxide synthase, inducible
    Polyclonal Antibody for Rat Nitric oxide synthase, inducible
    Polyclonal Antibody for Rat Nitric oxide synthase, inducible
    Cat.:
    P0837Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Rat Nitric oxide synthase, inducible
    Monoclonal Antibody for Rat Nitric oxide synthase, inducible
    Monoclonal Antibody for Rat Nitric oxide synthase, inducible
    Monoclonal Antibody for Rat Nitric oxide synthase, inducible
    Monoclonal Antibody for Rat Nitric oxide synthase, inducible
    Monoclonal Antibody for Rat Nitric oxide synthase, inducible
    Monoclonal Antibody for Rat Nitric oxide synthase, inducible
    Monoclonal Antibody for Rat Nitric oxide synthase, inducible
    Protein for Rat Nitric oxide synthase, inducible
    Protein for Rat Nitric oxide synthase, inducible
    Cat.:
    R0837h
    Price:
    Please sign in first.
    Packing:
    1mg
    Purity:
    >90%
    Protein for Rat Nitric oxide synthase, inducible
    Protein for Rat Nitric oxide synthase, inducible
    Protein for Rat Nitric oxide synthase, inducible
    Protein for Rat Nitric oxide synthase, inducible
    Protein for Rat Nitric oxide synthase, inducible
    Protein for Rat Nitric oxide synthase, inducible

    R&D Technical Data
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    s

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     12% SDS-PAGE analysis

                 12% SDS-PAGE analysis

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    s
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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    Recovery
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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    Saliva(n=3)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

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    Citations
    References
    1. 1.
      "Cloning of inducible nitric oxide synthase in rat vascular smooth muscle cells."
      Nunokawa Y. , Ishida N. , Tanaka S.
      Biochem. Biophys. Res. Commun.191:89-94(1993) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      tissue: Vascular smooth muscle.
    2. 2.
      "Cloning and expression of cytokine-inducible nitric oxide synthase cDNA from rat islets of Langerhans."
      Karlsen A.E. , Andersen H.U. , Vissing H. , Larsen P.M. , Fey S.J. , Cuartero B.G. , Madsen O.D. , Petersen J.S. , Mortensen S.B. , Mandrup-Poulsen T. , Boel E. , Nerup J.
      Diabetes44:753-758(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      strain: Wistar.
      tissue: Pancreatic islet.
    3. 3.
      "Cloning and expression of inducible nitric oxide synthase from rat astrocytes."
      Galea E. , Reis D.J. , Feinstein D.L.
      J. Neurosci. Res.37:406-414(1994) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      strain: Sprague-Dawley.
      tissue: Astrocyte.
    4. 4.
      "Molecular cloning of a cDNA encoding an inducible calmodulin-dependent nitric-oxide synthase from rat liver and its expression in COS 1 cells."
      Adachi H. , Iida S. , Oguchi S. , Ohshima H. , Suzuki H. , Nagasaki K. , Kawasaki H. , Sugimura T. , Esumi H.
      Eur. J. Biochem.217:37-43(1993) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PARTIAL PROTEIN SEQUENCE
      tissue: Liver.
    5. 5.
      "Hepatocytes and macrophages express an identical cytokine inducible nitric oxide synthase gene."
      Wood E.R. , Berger H. Jr. , Sherman P.A. , Lapetina E.G.
      Biochem. Biophys. Res. Commun.191:767-774(1993) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      strain: Sprague-Dawley.
      tissue: Hepatocyte.
    6. 6.
      "cDNA cloning and expression of inducible nitric oxide synthase from rat vascular smooth muscle cells."
      Geng Y.J. , Almquist M. , Hansson G.K.
      Biochim. Biophys. Acta1218:421-424(1994) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      strain: Sprague-Dawley.
      tissue: Aorta.
    7. 7.
      "Cloning of an inducible nitric oxide synthase from rat polymorphonuclear neutrophils."
      Kosuga K. , Yui Y. , Hattori R. , Sase K. , Eizawa H. , Aoyama T. , Inoue R. , Sasayama S.
      Endothelium2:217-221(1994)
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    8. 8.
      "Sequence analysis of inducible nitric oxide synthase in rat kidney, lung, and uterus."
      Tsutsumishita Y. , Kawai Y. , Takahara H. , Onda T. , Miyoshi J. , Futaki S. , Niwa M.
      Biol. Pharm. Bull.19:1374-1376(1996) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    9. 9.
      "Complete coding sequence of inducible nitric oxide synthase from human heart and skeletal muscle of patients with chronic heart failure."
      Adams V. , Krabbes S. , Jiang H. , Yu J. , Rahmel A. , Gielen S. , Schuler G. , Hambrecht R.
      Nitric Oxide2:242-249(1998) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    10. 10.
      "Vascular smooth muscle nitric oxide synthase anomalies in Dahl/Rapp salt-sensitive rats."
      Chen P.Y. , Gladish R.D. , Sanders P.W.
      Hypertension31:918-924(1998) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 426-788
      strain: Dahl/Rapp salt sensitive strain.
      tissue: Vascular smooth muscle.
    11. 11.
      "Advances in the studies of NO synthesis regulation in mesanglial cells."
      Saura M. , Zaragoza C. , Martinez-Dalmau R. , Perez-Sala D. , Lamas S.
      Nefrologia16:35-39(1996)
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 509-740
      strain: Wistar.
      tissue: Renal glomerulus.
    12. 12.
      "Location of an inducible nitric oxide synthase mRNA in the normal kidney."
      Morrissey J.J. , McCracken R. , Kaneto H. , Vehaskari M. , Montani D. , Klahr S.
      Kidney Int.45:998-1005(1994) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 479-655
      strain: Sprague-Dawley.
      tissue: Renal glomerulus.
    13. 13.
      "Isolation and characterization of iNOS from rat cardiocytes."
      Michel T. , Balligand J.-L.
      Submitted (1994-09) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 420-479
      tissue: Myocardium.
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