Platelet-derived growth factor A chain;PDGF-1;Platelet-derived growth factor alpha polypeptide;
Organism:
Rat (Rattus norvegicus).
General Annotation
Sub Unit:
Antiparallel disulfide-linked dimer of non-identical (A and B) chains. Homodimers of A and B chains are implicated in transformation processes. Interacts with CSPG4.
Function:
Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound.
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s
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Precision
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤6.8%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤11%
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Recovery
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Recovery was determined by spiking various levels of PDGFA into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
102
99-108
Plasma
101
96-103
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Linearity
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDGFA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
98-105%
97-107%
95-108%
99-107%
EDTA plasma(n=5)
97-106%
98-105%
98-105%
96-106%
heparin plasma(n=5)
99-105%
100-104%
96-107%
95-107%
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Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 58-196 (ISOFORM SHORT)