Interleukin-10 (Protein name
), IL10_MOUSE from NCBI database.
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General Annotation
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Antigen Annotation
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3D
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Predicted Eptitope
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Vaild Sequence
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Related Databases
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Citations (3)
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Gene name:
Il10(Il-10);
Protein name:
Interleukin-10(IL-10);
Alternative:
Cytokine synthesis inhibitory factor(CSIF);
Organism:
Mouse (Mus musculus).
General Annotation
Sub Unit:
Homodimer.
Function:
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
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12% SDS-PAGE Analysis
s
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.4%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤6.3%
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.2%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.0%
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.7%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤6.2%
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Recovery
Recovery was determined by spiking various levels of IL10 into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
102
99-105
Plasma
103
102-108
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Recovery was determined by spiking various levels of IL10 into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
99
94-103
Plasma
100
98-105
Recovery was determined by spiking various levels of IL10 into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
101
99-105
Plasma
102
100-104
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Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL10 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
100-105%
98-103%
107-111%
90-98%
EDTA plasma(n=5)
99-106%
103-105%
97-103%
101-104%
heparin plasma(n=5)
102-104%
90-98%
105-110%
87-95%
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL10 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
94-100%
97-102%
95-98%
101-105%
EDTA plasma(n=5)
98-104%
94-103%
102-108%
89-97%
heparin plasma(n=5)
85-96%
105-109%
98-103%
97-105%
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL10 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
100-104%
91-98%
99-106%
103-108%
EDTA plasma(n=5)
101-104%
98-103%
105-110%
93-100%
heparin plasma(n=5)
96-103%
89-97%
101-108%
104-107%
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