MMP9 Protein for Human(Homo sapiens) | EIAab.comMMP9 Protein for Human(Homo sapiens)

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MMP9 (Gene name),

Matrix metalloproteinase-9 (Protein name ), MMP9_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

Citations (3)

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Gene name:

MMP9(CLG4B);

Protein name:

Matrix metalloproteinase-9(MMP-9);

Alternative:

92 kDa type IV collagenase;92 kDa gelatinase;Gelatinase B(GELB);

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Exists as monomer or homodimer; disulfide-linked. Exists also as heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form. Interacts with ECM1.

Function:

May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.

Subcellular Location:

Secreted extracellular space extracellular matrix

Protein Attributes:

Sequence length:

707

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

600:

650:

700:

707:

ILQCPED

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

KEGG:

hsa:4318

String:

P14780

STRING:

9606.ENSP00000361405

SMR:

P14780

MIM:

120361

UniGene:

Hs.297413

Pfam:

PF00040

Uniprot:

P14780

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ELISA Kit for Human MMP-9

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E0553h

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96T

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ELISA Kit for Human MMP-9

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E0553m

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96T

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ELISA Kit for Human MMP-9

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E0553r

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96T

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ELISA Kit for Human MMP-9

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ELISA Kit for Human MMP-9

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E0553d

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96T

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ELISA Kit for Human MMP-9

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E0553b

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96T

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CLIA Kit for Human MMP-9

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CLIA Kit for Human MMP-9

Cat.:

U0553h

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96T

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CLIA Kit for Human MMP-9

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U0553r

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Looking for manual.

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96T

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CLIA Kit for Human MMP-9

Cat.:

U0553m

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96T

CLIA Kit for Human MMP-9

Cat.:

U0553b

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96T

CLIA Kit for Human MMP-9

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U0553d

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96T

Polyclonal Antibody for Human MMP-9

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P0553Rb-r

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40ug/0.2ml

Polyclonal Antibody for Human MMP-9

Cat.:

P0553Rb-h

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40ug/0.2ml

Polyclonal Antibody for Human MMP-9

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P0553Rb-m

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40ug/0.2ml

Polyclonal Antibody for Human MMP-9

Monoclonal Antibody for Human MMP-9

Protein for Human MMP-9

Cat.:

R0553h

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Packing:

1mg

Purity:

>90%

Protein for Human MMP-9

Cat.:

R0553m

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Packing:

1mg

Purity:

>90%

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.5

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.0

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of Human MMP9 into Human serum, plasma and cell culture media.

Sample Type	Average(%)	Recovery Range (%)
Serum	95	81-104
Plasma	96	89-102
Cell culture media	90	84-99

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

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Citations

[1].

"Erythropoietin attenuates ischemia–reperfusion induced lung injury by inhibiting tumor necrosis factor-α and matrix metalloproteinase-9 expression"

Haiwei Wu, Guohua Dong, Hao Liu, Biao Xu, Demin Li, Hua Jing

From:European Journal of Pharmacology

[2].

"Genetic relationship between serum pregnancy-associated plasma protein-A gene polymorphism and ischemic cerebrovascular disease in a Northern Han..."

H Wang, Y Song, C Zhang, et al.

From:中国神经再生研究 (英文版)

[3].

"Serum Levels of Matrix Metalloproteinases 2 and 9 in Patients with Acute Myocardial Infarction"

J Šímová, J Škvor, D Slovák, et al.

From:Folia Biologica (Praha)

References

1.

"SV40-transformed human lung fibroblasts secrete a 92-kDa type IV collagenase which is identical to that secreted by normal human macrophages."
Wilhelm S.M. , Collier I.E. , Marmer B.L. , Eisen A.Z. , Grant G.A. , Goldberg G.I.
J. Biol. Chem.264:17213-17221(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PROTEIN SEQUENCE OF 20-37;VARIANTS ARG-279 AND PRO-574
2.

"Complete structure of the human gene for 92-kDa type IV collagenase. Divergent regulation of expression for the 92- and 72-kilodalton enzyme genes in HT-1080 cells."
Huhtala P. , Tuuttila A. , Chow L.T. , Lohi J. , Keski-Oja J. , Tryggvason K.
J. Biol. Chem.266:16485-16490(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
3.

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA];VARIANT PRO-574
tissue: Umbilical cord blood.
4.

SeattleSNPs variation discovery resource
Submitted (2002-08) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANTS VAL-20; LYS-127; ARG-279; PRO-574 AND GLN-668
5.

NIEHS SNPs program
Submitted (2005-09) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANTS VAL-20; HIS-239; VAL-571; PRO-574 AND GLN-668
6.

"The DNA sequence and comparative analysis of human chromosome 20."
Deloukas P. , Matthews L.H. , Ashurst J.L. , Burton J. , Gilbert J.G.R. , Jones M. , Stavrides G. , Almeida J.P. , Babbage A.K. , Bagguley C.L. , Bailey J. , Barlow K.F. , Bates K.N. , Beard L.M. , Beare D.M. , Beasley O.P. , Bird C.P. , Blakey S.E. , more...
Nature414:865-871(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
7.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA];VARIANTS ARG-279 AND PRO-574
tissue: B-cell.
8.

"Regulatory mechanism of 92 kDa type IV collagenase gene expression which is associated with invasiveness of tumor cells."
Sato H. , Seiki M.
Oncogene8:395-405(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11
9.

"Human neutrophil gelatinase: a marker for circulating blood neutrophils. Purification and quantitation by enzyme linked immunosorbent assay."
Kjeldsen L. , Bjerrum O.W. , Hovgaard D. , Johnsen A.H. , Sehested M. , Borregaard N.
Eur. J. Haematol.49:180-191(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 20-39;GLYCOSYLATION
tissue: Neutrophil.
10.

"The cytokine-protease connection: identification of a 96-kD THP-1 gelatinase and regulation by interleukin-1 and cytokine inducers."
van Ranst M. , Norga K. , Masure S. , Proost P. , Vandekerckhove F. , Auwerx J. , van Damme J. , Opdenakker G.
Cytokine3:231-239(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 20-37
11.

"Matrix metalloproteinase 3 (stromelysin) activates the precursor for the human matrix metalloproteinase 9."
Ogata Y. , Enghild J.J. , Nagase H.
J. Biol. Chem.267:3581-3584(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 20-34; 60-71 AND 107-118;INDUCTION;PROTEOLYTIC PROCESSING BY MMP3
12.

"Matrix metalloproteinase 9 (92-kDa gelatinase/type IV collagenase) from HT 1080 human fibrosarcoma cells. Purification and activation of the precursor and enzymic properties."
Okada Y. , Gonoji Y. , Naka K. , Tomita K. , Nakanishi I. , Iwata K. , Yamashita K. , Hayakawa T.
J. Biol. Chem.267:21712-21719(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 20-32 AND 94-111;PROTEOLYTIC PROCESSING;INDUCTION
tissue: Fibrosarcoma.
13.

"Proteolytic and non-proteolytic activation of human neutrophil progelatinase B."
Sang Q.X. , Birkedal-Hansen H. , Van Wart H.E.
Biochim. Biophys. Acta1251:99-108(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 20-27; 60-67; 94-101 AND 107-113
14.

"Purification and identification of 91-kDa neutrophil gelatinase. Release by the activating peptide interleukin-8."
Masure S. , Proost P. , van Damme J. , Opdenakker G.
Eur. J. Biochem.198:391-398(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 28-60
tissue: Neutrophil.
15.

"Cytokine-mediated regulation of human leukocyte gelatinases and role in arthritis."
Opdenakker G. , Masure S. , Grillet B. , Van Damme J.
Lymphokine Cytokine Res.10:317-324(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 28-37
16.

"Latent collagenase and gelatinase from human neutrophils and their activation."
Tschesche H. , Knaeuper V. , Kraemer S. , Michaelis J. , Oberhoff R. , Reinke H.
Matrix Suppl.1:245-255(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 93-115;FUNCTION;CATALYTIC ACTIVITY
tissue: Blood.
17.

"Purification and characterization of human 92-kDa type IV collagenase (gelatinase B)."
Kang K. , Lee D.-H.
Exp. Mol. Med.28:161-165(1996)

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CHARACTERIZATION
18.

"Characterization of the monomeric and dimeric forms of latent and active matrix metalloproteinase-9. Differential rates for activation by stromelysin 1."
Olson M.W. , Bernardo M.M. , Pietila M. , Gervasi D.C. , Toth M. , Kotra L.P. , Massova I. , Mobashery S. , Fridman R.
J. Biol. Chem.275:2661-2668(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBUNIT
19.

"Salivary histatin 5 is an inhibitor of both host and bacterial enzymes implicated in periodontal disease."
Gusman H. , Travis J. , Helmerhorst E.J. , Potempa J. , Troxler R.F. , Oppenheim F.G.
Infect. Immun.69:1402-1408(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ENZYME REGULATION
20.

"Cleavage of metastasis suppressor gene product KiSS-1 protein/metastin by matrix metalloproteinases."
Takino T. , Koshikawa N. , Miyamori H. , Tanaka M. , Sasaki T. , Okada Y. , Seiki M. , Sato H.
Oncogene22:4617-4626(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEOLYTIC PROCESSING OF KISS1
21.

"Extracellular matrix protein 1 inhibits the activity of matrix metalloproteinase 9 through high-affinity protein/protein interactions."
Fujimoto N. , Terlizzi J. , Aho S. , Brittingham R. , Fertala A. , Oyama N. , McGrath J.A. , Uitto J.
Exp. Dermatol.15:300-307(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH ECM1;ENZYME REGULATION
22.

"A functional polymorphism in THBS2 that affects alternative splicing and MMP binding is associated with lumbar-disc herniation."
Hirose Y. , Chiba K. , Karasugi T. , Nakajima M. , Kawaguchi Y. , Mikami Y. , Furuichi T. , Mio F. , Miyake A. , Miyamoto T. , Ozaki K. , Takahashi A. , Mizuta H. , Kubo T. , Kimura T. , Tanaka T. , Toyama Y. , Ikegawa S.
Am. J. Hum. Genet.82:1122-1129(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INVOLVEMENT IN SUSCEPTIBILITY TO IDD
23.

"Mutations in MMP9 and MMP13 determine the mode of inheritance and the clinical spectrum of metaphyseal anadysplasia."
Lausch E. , Keppler R. , Hilbert K. , Cormier-Daire V. , Nikkel S. , Nishimura G. , Unger S. , Spranger J. , Superti-Furga A. , Zabel B.
Am. J. Hum. Genet.85:168-178(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INVOLVEMENT IN METAPHYSEAL ANADYSPLASIA TYPE 2
24.

"The adenomatous polyposis coli-associated exchange factors Asef and Asef2 are required for adenoma formation in Apc(Min/+)mice."
Kawasaki Y. , Tsuji S. , Muroya K. , Furukawa S. , Shibata Y. , Okuno M. , Ohwada S. , Akiyama T.
EMBO Rep.10:1355-1362(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INDUCTION
25.

"Structure of the C-terminally truncated human ProMMP9, a gelatin-binding matrix metalloproteinase."
Elkins P.A. , Ho Y.S. , Smith W.W. , Janson C.A. , D'Alessio K.J. , McQueney M.S. , Cummings M.D. , Romanic A.M.
Acta Crystallogr. D58:1182-1192(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 20-444 IN COMPLEX WITH ZINC AND MAGNESIUM IONS
26.

"Crystal structure of human MMP9 in complex with a reverse hydroxamate inhibitor."
Rowsell S. , Hawtin P. , Minshull C.A. , Jepson H. , Brockbank S.M.V. , Barratt D.G. , Slater A.M. , McPheat W.L. , Waterson D. , Henney A.M. , Pauptit R.A.
J. Mol. Biol.319:173-181(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 107-215 IN COMPLEX WITH INHIBITOR; ZINC AND CALCIUM IONS;MUTAGENESIS OF GLU-402
27.

"Structural basis of the adaptive molecular recognition by MMP9."
Cha H. , Kopetzki E. , Huber R. , Lanzendoerfer M. , Brandstetter H.
J. Mol. Biol.320:1065-1079(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 513-707;SUBUNIT
28.

"Theoretical model of human type IV collagenase precursor."
Mallena S.C. , Sagajkar R.D.
Submitted (2002-04) to the PDB data bank

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: 3D-STRUCTURE MODELING
29.

"Genetic variation at the matrix metalloproteinase-9 locus on chromosome 20q12.2-13.1."
Zhang B. , Henney A. , Eriksson P. , Hamsten A. , Watkins H. , Ye S.
Hum. Genet.105:418-423(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS VAL-20; LYS-82 AND ARG-279

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