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Index > Protein center > Timp1(Gene name) > Mouse
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  • Timp1 (Gene name),
  • Metalloproteinase inhibitor 1 (Protein name ),  TIMP1_MOUSE from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Gene name:
    Timp1(Timp;Timp-1);
    Protein name:
    Metalloproteinase inhibitor 1;
    Alternative:
    Erythroid-potentiating activity(EPA);Collagenase inhibitor 16C8 fibroblast;TPA-induced protein;TPA-S1;Tissue inhibitor of metalloproteinases 1(TIMP-1);
    Organism:
    Mouse (Mus musculus). 
    General Annotation
    Sub Unit:
    N/A
    Function:
    Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors.
    Subcellular Location:
    Secreted
    Protein Attributes:
    Sequence length:
    205
    Sequence:
    50:
    MMAPFASLAS | GILLLLSLIA | SSKACSCAPP | HPQTAFCNSD | LVIRAKFMGS | 
    100:
    PEINETTLYQ | RYKIKMTKML | KGFKAVGNAA | DIRYAYTPVM | ESLCGYAHKS | 
    150:
    QNRSEEFLIT | GRLRNGNLHI | SACSFLVPWR | TLSPAQQRAF | SKTYSAGCGV | 
    200:
    CTVFPCLSIP | CKLESDTHCL | WTDQVLVGSE | DYQSRHFACL | PRNPGLCTWR | 
    205:
    SLGAR
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
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    Related Databases
    Pfam:
    String:
    KEGG:
    SMR:
    UniGene:
    Uniprot:
     
    FOR
    ELISA Kit for Mouse Metalloproteinase inhibitor 1
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    E0552m
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    96T
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    ELISA Kit for Mouse Metalloproteinase inhibitor 1
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    E0552r
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    96T
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    ELISA Kit for Mouse Metalloproteinase inhibitor 1
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    ELISA Kit for Mouse Metalloproteinase inhibitor 1
    Cat.:
    E0552p
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    96T
    ELISA Kit for Mouse Metalloproteinase inhibitor 1
    Cat.:
    E0552b
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    96T
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    ELISA Kit for Mouse Metalloproteinase inhibitor 1
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    E0552d
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    ELISA Kit for Mouse Metalloproteinase inhibitor 1
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    E0552h
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    96T
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    CLIA Kit for Mouse Metalloproteinase inhibitor 1
    Cat.:
    U0552r
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    Packing:
    96T
    CLIA Kit for Mouse Metalloproteinase inhibitor 1
    MSDS:
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    CLIA Kit for Mouse Metalloproteinase inhibitor 1
    Cat.:
    U0552d
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Mouse Metalloproteinase inhibitor 1
    Cat.:
    U0552p
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Mouse Metalloproteinase inhibitor 1
    Cat.:
    U0552h
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    96T
    CLIA Kit for Mouse Metalloproteinase inhibitor 1
    Cat.:
    U0552b
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    MSDS:
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    96T
    CLIA Kit for Mouse Metalloproteinase inhibitor 1
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    U0552m
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    MSDS:
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    Packing:
    96T
    Polyclonal Antibody for Mouse Metalloproteinase inhibitor 1
    Cat.:
    P0552Rb-m
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse Metalloproteinase inhibitor 1
    Polyclonal Antibody for Mouse Metalloproteinase inhibitor 1
    Cat.:
    P0552Rb-h
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse Metalloproteinase inhibitor 1
    Polyclonal Antibody for Mouse Metalloproteinase inhibitor 1
    Polyclonal Antibody for Mouse Metalloproteinase inhibitor 1
    Polyclonal Antibody for Mouse Metalloproteinase inhibitor 1
    Cat.:
    P0552Rb-r
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    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Mouse Metalloproteinase inhibitor 1
    Monoclonal Antibody for Mouse Metalloproteinase inhibitor 1
    Monoclonal Antibody for Mouse Metalloproteinase inhibitor 1
    Monoclonal Antibody for Mouse Metalloproteinase inhibitor 1
    Monoclonal Antibody for Mouse Metalloproteinase inhibitor 1
    Monoclonal Antibody for Mouse Metalloproteinase inhibitor 1
    Monoclonal Antibody for Mouse Metalloproteinase inhibitor 1
    Protein for Mouse Metalloproteinase inhibitor 1
    Protein for Mouse Metalloproteinase inhibitor 1
    Protein for Mouse Metalloproteinase inhibitor 1
    Protein for Mouse Metalloproteinase inhibitor 1
    Protein for Mouse Metalloproteinase inhibitor 1
    Protein for Mouse Metalloproteinase inhibitor 1
    Cat.:
    R0552h
    Price:
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    Packing:
    1mg
    Purity:
    >90%
    Protein for Mouse Metalloproteinase inhibitor 1
    Cat.:
    R0552m
    Price:
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    Packing:
    1mg
    Purity:
    >90%

    R&D Technical Data
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              12% SDS-PAGE Analysis

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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤ 5.8%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤ 8.9%

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    Recovery
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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    92-105

    Plasma

    98

    91-106

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    90-103%

    91-104%

    94-102%

    92-105%

    EDTA plasma(n=5)

    91-97%

    93-102%

    89-105%

    91-106%

    heparin plasma(n=5)

    92-104%

     

    94-108%

    95-109%

    91-107%

     

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    References
    1. 1.
      "Characterization and expression of a murine gene homologous to human EPA/TIMP: a virus-induced gene in the mouse."
      Gewert D.R. , Coulombe B. , Castelino M. , Skup D. , Williams B.R.G.
      EMBO J.6:651-657(1987) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    2. 2.
      "A growth-responsive gene (16C8) in normal mouse fibroblasts homologous to a human collagenase inhibitor with erythroid-potentiating activity: evidence for inducible and constitutive transcripts."
      Edwards D.R. , Waterhouse P. , Holman M.L. , Denhardt D.T.
      Nucleic Acids Res.14:8863-8878(1986) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      tissue: Fibroblast.
    3. 3.
      "Molecular cloning of gene sequences regulated by tumor promoters and mitogens through protein kinase C."
      Johnson M.D. , Housey G.M. , Kirschmeier P.T. , Weinstein I.B.
      Mol. Cell. Biol.7:2821-2829(1987) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      strain: C3H.
    4. 4.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      strain: FVB/N.
      tissue: Mammary gland.
      tissue: Osteoblast.
    5. 5.
      "Genes for extracellular-matrix-degrading metalloproteinases and their inhibitor, TIMP, are expressed during early mammalian development."
      Brenner C.A. , Adler R.R. , Rappolee D.A. , Pedersen R.A. , Werb Z.
      Genes Dev.3:848-859(1989) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PARTIAL NUCLEOTIDE SEQUENCE;DEVELOPMENTAL STAGE
      tissue: Embryo.
    6. 6.
      "Molecular cloning of partial cDNA copies of two distinct mouse IFN-beta mRNAs."
      Skup D. , Windass J.D. , Sor F.S. , George H. , Williams B.R. , Fukuhara H. , de Maeyer-Guignard J. , de Maeyer E.
      Nucleic Acids Res.10:3069-3084(1982) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE OF 168-205
    7. 7.
      "Timp1 interacts with beta-1 integrin and CD63 along melanoma genesis and confers anoikis resistance by activating PI3-K signaling pathway independently of Akt phosphorylation."
      Toricelli M. , Melo F.H. , Peres G.B. , Silva D.C. , Jasiulionis M.G.
      Mol. Cancer12:22-22(2013) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION;INTERACTION WITH CD63;IDENTIFICATION IN A COMPLEX WITH CD63 AND ITGB1
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