Gene name:
SRSF1 (OK/SW-cl.3;ASF;SF2;SF2P33;SFRS1) ;
Protein name:
Serine/arginine-rich splicing factor 1 ;
Alternative:
Splicing factor, arginine/serine-rich 1 ;Alternative-splicing factor 1 (ASF-1) ;pre-mRNA-splicing factor SF2, P33 subunit ;
Organism:
Human (Homo sapiens).
General Annotation
Sub Unit:
Consists of two polypeptides of p32 and p33. In vitro, self-associates and binds SRSF2, SNRNP70 and U2AF1 but not U2AF2. Binds SREK1/SFRS12. Interacts with SAFB/SAFB1. Interacts with PSIP1/LEDGF. Interacts with SRPK1. Identified in the spliceosome C complex. Interacts with RSRC1 (via Arg/Ser-rich domain). Interacts with ZRSR2/U2AF1-RS2. Interacts with CCDC55 (via C-terminus).
Function:
Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors.
Subcellular Location:
Cytoplasm
Nucleus speckle
In nuclear speckles. Shuttles between the nucleus and the cytoplasm.
Protein Attributes:
50:
MSGGGVIRGP | AGNNDCRIYV | GNLPPDIRTK | DIEDVFYKYG | AIRDIDLKNR |
100:
RGGPPFAFVE | FEDPRDAEDA | VYGRDGYDYD | GYRLRVEFPR | SGRGTGRGGG |
150:
GGGGGGAPRG | RYGPPSRRSE | NRVVVSGLPP | SGSWQDLKDH | MREAGDVCYA |
200:
DVYRDGTGVV | EFVRKEDMTY | AVRKLDNTKF | RSHEGETAYI | RVKVDGPRSP |
248:
SYGRSRSRSR | SRSRSRSRSN | SRSRSYSPRR | SRGSPRYSPR | HSRSRSRT
Vaild Sequence:
Related Databases
Uniprot:
ELISA Kit
CLIA Kit
Polyclonal Antibody
Monoclonal Antibody
Protein
FOR
Human
Chicken
Mouse
Pig
Bovine
ELISA Kit for Human Serine/arginine-rich splicing factor 1
ELISA Kit for Human Serine/arginine-rich splicing factor 1
ELISA Kit for Human Serine/arginine-rich splicing factor 1
ELISA Kit for Human Serine/arginine-rich splicing factor 1
ELISA Kit for Human Serine/arginine-rich splicing factor 1
CLIA Kit for Human Serine/arginine-rich splicing factor 1
CLIA Kit for Human Serine/arginine-rich splicing factor 1
CLIA Kit for Human Serine/arginine-rich splicing factor 1
CLIA Kit for Human Serine/arginine-rich splicing factor 1
CLIA Kit for Human Serine/arginine-rich splicing factor 1
Polyclonal Antibody for Human Serine/arginine-rich splicing factor 1
Polyclonal Antibody for Human Serine/arginine-rich splicing factor 1
Polyclonal Antibody for Human Serine/arginine-rich splicing factor 1
Polyclonal Antibody for Human Serine/arginine-rich splicing factor 1
Polyclonal Antibody for Human Serine/arginine-rich splicing factor 1
Monoclonal Antibody for Human Serine/arginine-rich splicing factor 1
Monoclonal Antibody for Human Serine/arginine-rich splicing factor 1
Monoclonal Antibody for Human Serine/arginine-rich splicing factor 1
Monoclonal Antibody for Human Serine/arginine-rich splicing factor 1
Monoclonal Antibody for Human Serine/arginine-rich splicing factor 1
Protein for Human Serine/arginine-rich splicing factor 1
Protein for Human Serine/arginine-rich splicing factor 1
Protein for Human Serine/arginine-rich splicing factor 1
Protein for Human Serine/arginine-rich splicing factor 1
Protein for Human Serine/arginine-rich splicing factor 1
R&D Technical Data
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Precision
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Recovery
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Linearity
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Citations
[1].
J Sokalski, D Trzybulska, M Bodnar, et al.
References
1.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ASF-1 AND ASF-2);PARTIAL PROTEIN SEQUENCE;ALTERNATIVE SPLICING
2.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ASF-1);PROTEIN SEQUENCE OF 144-161 AND 167-175
3.
"Identification of immuno-peptidmics that are recognized by tumor-reactive CTL generated from TIL of colon cancer patients."
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Itoh K.
Submitted (2001-05) to the EMBL/GenBank/DDBJ databases
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM ASF-1)
tissue :
Colon adenocarcinoma .
4.
"Complete sequencing and characterization of 21,243 full-length human cDNAs."
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more...
Nat. Genet.36:40-45(2004)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM ASF-1)
tissue :
Testis .
5.
Mural R.J.
,
Istrail S.
,
Sutton G.G.
,
Florea L.
,
Halpern A.L.
,
Mobarry C.M.
,
Lippert R.
,
Walenz B.
,
Shatkay H.
,
Dew I.
,
Miller J.R.
,
Flanigan M.J.
,
Edwards N.J.
,
Bolanos R.
,
Fasulo D.
,
Halldorsson B.V.
,
Hannenhalli S.
,
Turner R.
,
Yooseph S.
,
Lu F.
,
Nusskern D.R.
,
Shue B.C.
,
Zheng X.H.
,
Zhong F.
,
Delcher A.L.
,
Huson D.H.
,
Kravitz S.A.
,
Mouchard L.
,
Reinert K.
,
Remington K.A.
,
Clark A.G.
,
Waterman M.S.
,
Eichler E.E.
,
Adams M.D.
,
Hunkapiller M.W.
,
Myers E.W.
,
Venter J.C.
more...
Submitted (2005-09) to the EMBL/GenBank/DDBJ databases
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
6.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS ASF-1 AND ASF-3)
tissue :
Brain .
tissue :
Placenta .
7.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : PROTEIN SEQUENCE OF 18-28; 31-38; 52-65; 75-83; 123-138 AND 143-164;IDENTIFICATION BY MASS SPECTROMETRY
tissue :
Fetal brain cortex .
8.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : PROTEIN SEQUENCE OF 123-140
9.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : CHARACTERIZATION
10.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : INTERACTION IN SPLICEOSOME ASSEMBLY
11.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : MUTAGENESIS;CHARACTERIZATION OF FUNCTIONAL DOMAINS
12.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : FUNCTION IN RECRUITMENT OF U1-70K TO PRE-MRNA
13.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : RECOGNITION OF PRE-MRNA 5'SPLICE SITES
14.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : RNA-BINDING SPECIFICITY
15.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : INTERACTION WITH ZRSR2
16.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : SUBCELLULAR LOCATION;MUTAGENESIS OF PHE-162 AND PHE-180
17.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : INTERACTION WITH PSIP1
18.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : INTERACTION WITH SAFB/SAFB1
19.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : INTERACTION WITH SREK1
20.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : SUBCELLULAR LOCATION
21.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : IDENTIFICATION BY MASS SPECTROMETRY;IDENTIFICATION IN THE SPLICEOSOMAL C COMPLEX
22.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : INTERACTION WITH NXF1
23.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : PHOSPHORYLATION AT SERINE RESIDUES;SUBCELLULAR LOCATION;INTERACTION WITH SRPK1
24.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : INTERACTION WITH RSRC1
25.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue :
Cervix carcinoma .
26.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : PHOSPHORYLATION BY SRPK1;THE MECHANISM OF PHOSPHORYATION
27.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : PHOSPHORYLATION BY SRPK1;THE MECHANISM OF PHOSPHORYATION
28.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199; SER-205; SER-231; SER-234 AND SER-238;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue :
Cervix carcinoma .
29.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : PHOSPHORYLATION BY SRPK1;THE MECHANISM OF PHOSPHORYATION
30.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : PHOSPHORYLATION BY SRPK1;THE MECHANISM OF PHOSPHORYATION
31.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : RNA-BINDING
32.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-38 AND LYS-179;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
33.
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Cited for : METHYLATION AT ARG-93; ARG-97 AND ARG-109;MUTAGENESIS OF ARG-93; ARG-97 AND ARG-109;SUBCELLULAR LOCATION
34.
"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V.
,
Vermeulen M.
,
Santamaria A.
,
Kumar C.
,
Miller M.L.
,
Jensen L.J.
,
Gnad F.
,
Cox J.
,
Jensen T.S.
,
Nigg E.A.
,
Brunak S.
,
Mann M.
Sci. Signal.3:RA3-RA3(2010)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
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Cited for : PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue :
Cervix carcinoma .
35.
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Cited for : IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
36.
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Cited for : INTERACTION WITH CCDC55
37.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2;PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2 AND SER-199;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS];CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS]
38.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : STRUCTURE BY NMR OF 1-98
39.
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Cited for : X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 105-219 IN COMPLEX WITH SRPK1;MECHANISM OF ITS PHOSPHORYLATION BY SRPK1;DOMAIN RRM
40.
"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T.
,
Jones S.
,
Wood L.D.
,
Parsons D.W.
,
Lin J.
,
Barber T.D.
,
Mandelker D.
,
Leary R.J.
,
Ptak J.
,
Silliman N.
,
Szabo S.
,
Buckhaults P.
,
Farrell C.
,
Meeh P.
,
Markowitz S.D.
,
Willis J.
,
Dawson D.
,
Willson J.K.V.
,
Gazdar A.F.
,
Hartigan J.
,
Wu L.
,
Liu C.
,
Parmigiani G.
,
Park B.H.
,
Bachman K.E.
,
Papadopoulos N.
,
Vogelstein B.
,
Kinzler K.W.
,
Velculescu V.E.
more...
Science314:268-274(2006)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
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Cited for : VARIANT [LARGE SCALE ANALYSIS] SER-89