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Index > Protein center > Tgfa(Gene name) > Rat
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  • Tgfa (Gene name),
  • Protransforming growth factor alpha (Protein name ),  TGFA_RAT from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    Tgfa;
    Protein name:
    Protransforming growth factor alpha;
    Alternative:

    Organism:
    Rat (Rattus norvegicus). 
    General Annotation
    Sub Unit:
    Interacts with the PDZ domains of MAGI3, SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2.
    Function:
    TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
    Subcellular Location:
    Protransforming growth factor alpha Cell membrane Single-pass type I membrane protein
    Protein Attributes:
    Sequence length:
    159
    Sequence:
    50:
    MVPAAGQLAL | LALGILVAVC | QALENSTSPL | SDSPVAAAVV | SHFNKCPDSH | 
    100:
    TQYCFHGTCR | FLVQEEKPAC | VCHSGYVGVR | CEHADLLAVV | AASQKKQAIT | 
    150:
    ALVVVSIVAL | AVLIITCVLI | HCCQVRKHCE | WCRALVCRHE | KPSALLKGRT | 
    159:
    ACCHSETVV
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
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    Related Databases
    UniGene:
    SMR:
    String:
    KEGG:
    Uniprot:
     
    FOR
    ELISA Kit for Rat Protransforming growth factor alpha
    Cat.:
    E0123m
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    Packing:
    96T
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    ELISA Kit for Rat Protransforming growth factor alpha
    Cat.:
    E0123h
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    MSDS:
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    Packing:
    96T
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    ELISA Kit for Rat Protransforming growth factor alpha
    Cat.:
    E0123r
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    MSDS:
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    Packing:
    96T
    Range:
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    ELISA Kit for Rat Protransforming growth factor alpha
    MSDS:
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    ELISA Kit for Rat Protransforming growth factor alpha
    Cat.:
    E0123p
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Rat Protransforming growth factor alpha
    Cat.:
    U0123p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat Protransforming growth factor alpha
    Cat.:
    U0123h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat Protransforming growth factor alpha
    MSDS:
    Please sign in first.
    CLIA Kit for Rat Protransforming growth factor alpha
    Cat.:
    U0123r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat Protransforming growth factor alpha
    Cat.:
    U0123m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Rat Protransforming growth factor alpha
    Polyclonal Antibody for Rat Protransforming growth factor alpha
    Cat.:
    P0123Rb-m
    Price:
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat Protransforming growth factor alpha
    Cat.:
    P0123Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat Protransforming growth factor alpha
    Polyclonal Antibody for Rat Protransforming growth factor alpha
    Cat.:
    P0123Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Rat Protransforming growth factor alpha
    Monoclonal Antibody for Rat Protransforming growth factor alpha
    Monoclonal Antibody for Rat Protransforming growth factor alpha
    Monoclonal Antibody for Rat Protransforming growth factor alpha
    Monoclonal Antibody for Rat Protransforming growth factor alpha
    Protein for Rat Protransforming growth factor alpha
    Protein for Rat Protransforming growth factor alpha
    Protein for Rat Protransforming growth factor alpha
    Protein for Rat Protransforming growth factor alpha
    Protein for Rat Protransforming growth factor alpha

    R&D Technical Data
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    s
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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤6.1%

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    Recovery
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    Recovery was determined by spiking various levels of TGFA into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    98

    95-102

    Plasma

    97

    93-104

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TGFA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    95-100%

    96-103%

    94-99%

    94-101%

    EDTA plasma(n=5)

    96-105%

    90-98%

    92-101%

    93-104%

    heparin plasma(n=5)

    92-101%

     

    95-99%

    97-102%

    96-103%

     

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    References
    1. 1.
      "Cloning and sequence analysis of a cDNA for rat transforming growth factor-alpha."
      Lee D.C. , Rose T.M. , Webb N.R. , Todaro G.J.
      Nature313:489-491(1985) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    2. 2.
      "Characterization of the rat transforming growth factor alpha gene and identification of promoter sequences."
      Blasband A.J. , Rogers K.T. , Chen X. , Azizkhan J.C. , Lee D.C.
      Mol. Cell. Biol.10:2111-2121(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]
    3. 3.
      "Rat transforming growth factor type 1: structure and relation to epidermal growth factor."
      Marquardt H. , Hunkapiller M.W. , Hood L.E. , Todaro G.J.
      Science223:1079-1082(1984) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 39-88
    4. 4.
      "Epidermal growth factor-like transforming growth factor. I. Isolation, chemical characterization, and potentiation by other transforming factors from feline sarcoma virus-transformed rat cells."
      Massague J.
      J. Biol. Chem.258:13606-13613(1983) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 39-67
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