Protransforming growth factor alpha (Protein name
), TGFA_RAT from NCBI database.
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General Annotation
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Antigen Annotation
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3D
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Predicted Eptitope
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Vaild Sequence
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Related Databases
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Gene name:
Tgfa;
Protein name:
Protransforming growth factor alpha;
Alternative:
Organism:
Rat (Rattus norvegicus).
General Annotation
Sub Unit:
Interacts with the PDZ domains of MAGI3, SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2.
Function:
TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
Subcellular Location:
Protransforming growth factor alpha
Cell membrane
Single-pass type I membrane protein
Monoclonal Antibody for Rat Protransforming growth factor alpha
Monoclonal Antibody for Rat Protransforming growth factor alpha
Monoclonal Antibody for Rat Protransforming growth factor alpha
Monoclonal Antibody for Rat Protransforming growth factor alpha
Monoclonal Antibody for Rat Protransforming growth factor alpha
Protein for Rat Protransforming growth factor alpha
Protein for Rat Protransforming growth factor alpha
Protein for Rat Protransforming growth factor alpha
Protein for Rat Protransforming growth factor alpha
Protein for Rat Protransforming growth factor alpha
R&D Technical Data
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s
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Precision
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤3.9%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤6.1%
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Recovery
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Recovery was determined by spiking various levels of TGFA into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
98
95-102
Plasma
97
93-104
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Linearity
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TGFA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1：2
1:4
1:8
1:16
serum(n=5)
95-100%
96-103%
94-99%
94-101%
EDTA plasma(n=5)
96-105%
90-98%
92-101%
93-104%
heparin plasma(n=5)
92-101%
95-99%
97-102%
96-103%
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Vaild Sequence:
