TNF ELISA Kit for Pig(Sus scrofa) | EIAab.comTNF ELISA Kit for Pig(Sus scrofa)

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TNF (Gene name),

Tumor necrosis factor (Protein name ), TNFA_PIG from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

Citations (1)

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Gene name:

TNF(TNFA;TNFSF2);

Protein name:

Tumor necrosis factor;

Alternative:

TNF-alpha;Cachectin;Tumor necrosis factor ligand superfamily member 2(TNF-a);

Organism:

Pig (Sus scrofa).

General Annotation

Sub Unit:

Homotrimer.

Function:

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

Subcellular Location:

Tumor necrosis factor, soluble form Secreted

Protein Attributes:

Sequence length:

232

Sequence:

50:

100:

150:

200:

232:

LEKDDRLSAE | INLPDYLDFA | ESGQVYFGII | AL

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

Pfam:

PF00229

UniGene:

Ssc.100

SMR:

P23563

STRING:

9823.ENSSSCP00000001491

String:

P23563

KEGG:

ssc:397086

Uniprot:

P23563

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ELISA Kit for Pig Tumor necrosis factor

Cat.:

E0133h

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Packing:

96T

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ELISA Kit for Pig Tumor necrosis factor

Cat.:

E0133d

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Manual:

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Packing:

96T

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ELISA Kit for Pig Tumor necrosis factor

Cat.:

E0133p

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96T

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ELISA Kit for Pig Tumor necrosis factor

MSDS:

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ELISA Kit for Pig Tumor necrosis factor

Cat.:

E0133m

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Packing:

96T

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ELISA Kit for Pig Tumor necrosis factor

Cat.:

E0133r

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96T

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ELISA Kit for Pig Tumor necrosis factor

Cat.:

E0133b

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Looking for manual.

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96T

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CLIA Kit for Pig Tumor necrosis factor

Cat.:

U0133b

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Looking for manual.

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Packing:

96T

Range:

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CLIA Kit for Pig Tumor necrosis factor

Cat.:

U0133p

Price:

Please sign in first.

MSDS:

Please sign in first.

Packing:

96T

CLIA Kit for Pig Tumor necrosis factor

Cat.:

U0133m

Price:

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Manual:

Looking for manual.

MSDS:

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Packing:

96T

Range:

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CLIA Kit for Pig Tumor necrosis factor

MSDS:

Please sign in first.

CLIA Kit for Pig Tumor necrosis factor

Cat.:

U0133h

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Packing:

96T

CLIA Kit for Pig Tumor necrosis factor

Cat.:

U0133d

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96T

CLIA Kit for Pig Tumor necrosis factor

Cat.:

U0133r

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Packing:

96T

Polyclonal Antibody for Pig Tumor necrosis factor

Cat.:

P0133Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Pig Tumor necrosis factor

Cat.:

P0133Rb-h

Price:

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Packing:

40ug/0.2ml

Polyclonal Antibody for Pig Tumor necrosis factor

Cat.:

P0133Rb-r

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Packing:

40ug/0.2ml

Monoclonal Antibody for Pig Tumor necrosis factor

Protein for Pig Tumor necrosis factor

Cat.:

R0133h

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Packing:

1mg

Purity:

>90%

Protein for Pig Tumor necrosis factor

Cat.:

R0133m

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Packing:

1mg

Purity:

>90%

Protein for Pig Tumor necrosis factor

Cat.:

R0133r

Price:

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Packing:

1mg

Purity:

>90%

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.4%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.0%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of TNF into serum and plasma .

Sample Type	Average(%)	Recovery Range (%)
Serum	102	95-114
Plasma	96	93-99

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of TNF into serum and plasma .

Sample Type	Average(%)	Recovery Range (%)
Serum	89	86-104
Plasma	95	86-105

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-101%	96-105%	98-104%	98-107%
EDTA plasma(n=5)	95-103%	97-107%	100-110%	88-103%
heparin plasma(n=5)	98-103%	96-102%	93-104%	95-115%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-103%	90-101%	98-109%	97-110%
EDTA plasma(n=5)	101-105%	97-109%	89-107%	99-108%
heparin plasma(n=5)	98-107%	96-110%	100-112%	101-116%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Citations

[1].

"Propofol Inhibits the Activation of p38 through Up-Regulating the Expression of Annexin A1 to Exert Its Anti-Inflammation Effect"

Jing Tang, Xi Chen, Weifeng Tu, Yuanbo Guo, Zhenlong Zhao, Qiong Xue, Chunshui Lin, Jinfang Xiao, Xuegang Sun, Tao Tao, Miaoning Gu, Youtan Liu

From:Plos ONE

References

1.

"Gene sequence of porcine tumor necrosis factor alpha."
Drews R.T. , Coffee B.W. , Prestwood A.K. , McGraw R.A.
Nucleic Acids Res.18:5564-5564(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
2.

"The porcine tumor necrosis factor-encoding genes: sequence and comparative analysis."
Kuhnert P. , Wuethrich C. , Peterhans E. , Pauli U.
Gene102:171-178(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
tissue: Liver.
3.

"Complete nucleotide sequence of a cDNA encoding porcine tumor necrosis factor-alpha."
Choi C.S. , Molitor T.W. , Lin G.F. , Murtaugh M.P.
Anim. Biotechnol.2:97-105(1991)

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
tissue: Macrophage.
4.

"Sequence of the swine major histocompatibility complex region containing all non-classical class I genes."
Chardon P. , Rogel-Gaillard C. , Cattolico L. , Duprat S. , Vaiman M. , Renard C.
Tissue Antigens57:55-65(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
strain: Large white.
tissue: Fibroblast.
5.

Porcine genome sequencing project
Submitted (2007-05) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
6.

"Porcine tumor necrosis factor alpha: cloning with the polymerase chain reaction and determination of the nucleotide sequence."
Pauli U. , Beutler B. , Peterhans E.
Gene81:185-191(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 44-232

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