UBA3 ELISA Kit for Human(Homo sapiens) | EIAab.comUBA3 ELISA Kit for Human(Homo sapiens)

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Index > Protein center > UBA3(Gene name) > Human

UBA3 (Gene name),

NEDD8-activating enzyme E1 catalytic subunit (Protein name ), UBA3_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

UBA3(UBE1C);

Protein name:

NEDD8-activating enzyme E1 catalytic subunit;

Alternative:

Ubiquitin-activating enzyme E1C;NEDD8-activating enzyme E1C;Ubiquitin-like modifier-activating enzyme 3(Ubiquitin-activating enzyme 3);

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Heterodimer of UBA3 and NAE1. Interacts with NEDD8, UBE2F and UBE2M. Binds ESR1 and ESR2 with bound steroid ligand.

Function:

Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression.

Subcellular Location:

N/A

Protein Attributes:

Sequence length:

463

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

463:

TPQTVLFKLH | FTS

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

String:

Q8TBC4

UniGene:

Hs.154320

KEGG:

hsa:9039

MIM:

603172

Pfam:

PF08825

SMR:

Q8TBC4

STRING:

9606.ENSP00000354340

Uniprot:

Q8TBC4

FOR

ELISA Kit for Human NEDD8-activating enzyme E1 catalytic subunit

Cat.:

E1027h

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96T

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ELISA Kit for Human NEDD8-activating enzyme E1 catalytic subunit

Cat.:

E1027m

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Packing:

96T

ELISA Kit for Human NEDD8-activating enzyme E1 catalytic subunit

Cat.:

E1027r

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96T

CLIA Kit for Human NEDD8-activating enzyme E1 catalytic subunit

Cat.:

U1027r

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Packing:

96T

CLIA Kit for Human NEDD8-activating enzyme E1 catalytic subunit

Cat.:

U1027h

Price:

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Manual:

Looking for manual.

MSDS:

Please sign in first.

Packing:

96T

Range:

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CLIA Kit for Human NEDD8-activating enzyme E1 catalytic subunit

Cat.:

U1027m

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MSDS:

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Packing:

96T

Polyclonal Antibody for Human NEDD8-activating enzyme E1 catalytic subunit

Cat.:

P1027Rb-m

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40ug/0.2ml

Polyclonal Antibody for Human NEDD8-activating enzyme E1 catalytic subunit

Cat.:

P1027Rb-r

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40ug/0.2ml

Polyclonal Antibody for Human NEDD8-activating enzyme E1 catalytic subunit

Cat.:

P1027Rb-h

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Packing:

40ug/0.2ml

Monoclonal Antibody for Human NEDD8-activating enzyme E1 catalytic subunit

Protein for Human NEDD8-activating enzyme E1 catalytic subunit

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

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Linearity

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References

1.

"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs."
Wiemann S. , Weil B. , Wellenreuther R. , Gassenhuber J. , Glassl S. , Ansorge W. , Boecher M. , Bloecker H. , Bauersachs S. , Blum H. , Lauber J. , Duesterhoeft A. , Beyer A. , Koehrer K. , Strack N. , Mewes H.-W. , Ottenwaelder B. , Obermaier B. , more...
Genome Res.11:422-435(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
tissue: Fetal kidney.
2.

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2)
3.

"The DNA sequence, annotation and analysis of human chromosome 3."
Muzny D.M. , Scherer S.E. , Kaul R. , Wang J. , Yu J. , Sudbrak R. , Buhay C.J. , Chen R. , Cree A. , Ding Y. , Dugan-Rocha S. , Gill R. , Gunaratne P. , Harris R.A. , Hawes A.C. , Hernandez J. , Hodgson A.V. , Hume J. , more...
Nature440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
4.

Mural R.J. , Istrail S. , Sutton G.G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
5.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1);VARIANT ARG-9
tissue: Prostatic adenocarcinoma.
6.

"Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway."
Gong L. , Yeh E.T.H.
J. Biol. Chem.274:12036-12042(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-463 (ISOFORM 1);VARIANT ARG-9;FUNCTION;MUTAGENESIS OF CYS-237;TISSUE SPECIFICITY
tissue: Placenta.
7.

"A new NEDD8-ligating system for cullin-4A."
Osaka F. , Kawasaki H. , Aida N. , Saeki M. , Chiba T. , Kawashima S. , Tanaka K. , Kato S.
Genes Dev.12:2263-2268(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 22-463;FUNCTION
8.

"Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer."
Bohnsack R.N. , Haas A.L.
J. Biol. Chem.278:26823-26830(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH NAE1
9.

"Initial characterization of the human central proteome."
Burkard T.R. , Planyavsky M. , Kaupe I. , Breitwieser F.P. , Buerckstuemmer T. , Bennett K.L. , Superti-Furga G. , Colinge J.
BMC Syst. Biol.5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
10.

"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P. , Lasa M. , Polevoda B. , Gazquez C. , Elosegui-Artola A. , Kim D.S. , De Juan-Pardo E. , Demeyer K. , Hole K. , Larrea E. , Timmerman E. , Prieto J. , Arnesen T. , Sherman F. , Gevaert K. , Aldabe R.
Proc. Natl. Acad. Sci. U.S.A.109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS];CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS]
11.

"The structure of the APPBP1-UBA3-NEDD8-ATP complex reveals the basis for selective ubiquitin-like protein activation by an E1."
Walden H. , Podgorski M.S. , Huang D.T. , Miller D.W. , Howard R.J. , Minor D.L. Jr. , Holton J.M. , Schulman B.A.
Mol. Cell12:1427-1437(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 33-463 IN COMPLEX WITH NAE1; NEDD8 AND ATP;MUTAGENESIS OF ARG-211
12.

"Insights into the ubiquitin transfer cascade from the structure of the activating enzyme for NEDD8."
Walden H. , Podgorski M.S. , Schulman B.A.
Nature422:330-334(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 24-463 IN COMPLEX WITH NAE1;MUTAGENESIS OF ILE-148; ASP-167; 227-LEU-TYR-228; THR-238; ILE-310 AND 352-TYR--TYR-357
13.

"A unique E1-E2 interaction required for optimal conjugation of the ubiquitin-like protein NEDD8."
Huang D.T. , Miller D.W. , Mathew R. , Cassell R. , Holton J.M. , Roussel M.F. , Schulman B.A.
Nat. Struct. Mol. Biol.11:927-935(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 33-463 IN COMPLEX WITH NAE1 AND UBE2M;MUTAGENESIS OF PHE-65; 160-HIS-ILE-161; PRO-192; ILE-195; PRO-197; LEU-214; MET-217 AND ILE-331
14.

"Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1."
Huang D.T. , Paydar A. , Zhuang M. , Waddell M.B. , Holton J.M. , Schulman B.A.
Mol. Cell17:341-350(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 368-463 IN COMPLEX WITH UBE2M;MUTAGENESIS OF SER-368; GLN-369; LEU-370; THR-412; LEU-415; VAL-418; ILE-421 AND ARG-424
15.

"E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification."
Huang D.T. , Ayrault O. , Hunt H.W. , Taherbhoy A.M. , Duda D.M. , Scott D.C. , Borg L.A. , Neale G. , Murray P.J. , Roussel M.F. , Schulman B.A.
Mol. Cell33:483-495(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 368-420 IN COMPLEX WITH UBE2F

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