Vegfa ELISA Kit for Mouse(Mus musculus) | EIAab.comVegfa ELISA Kit for Mouse(Mus musculus)

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Vegfa (Gene name),

Vascular endothelial growth factor A (Protein name ), VEGFA_MOUSE from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

Citations (1)

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Gene name:

Vegfa(Vegf);

Protein name:

Vascular endothelial growth factor A(VEGF-A);

Alternative:

Vascular permeability factor(VPF);

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

Homodimer; disulfide-linked. Also found as heterodimer with PGF.

Function:

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis.

Subcellular Location:

Isoform VEGF-3 Cell membrane Peripheral membrane protein Remains cell-surface associated unless released by heparin.

Protein Attributes:

Sequence length:

214

Sequence:

50:

100:

150:

200:

214:

ELNERTCRCD | KPRR

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

UniGene:

Mm.282184

String:

Q00731

KEGG:

mmu:22339

Pfam:

PF00341

SMR:

Q00731

Uniprot:

Q00731

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ELISA Kit for Mouse VEGF-A

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E0143h

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ELISA Kit for Mouse VEGF-A

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E0143r

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ELISA Kit for Mouse VEGF-A

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E0143p

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ELISA Kit for Mouse VEGF-A

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E0143c

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ELISA Kit for Mouse VEGF-A

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E0143d

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ELISA Kit for Mouse VEGF-A

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E0143m

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ELISA Kit for Mouse VEGF-A

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E0143b

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CLIA Kit for Mouse VEGF-A

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U0143b

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96T

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CLIA Kit for Mouse VEGF-A

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U0143d

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CLIA Kit for Mouse VEGF-A

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U0143p

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CLIA Kit for Mouse VEGF-A

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U0143r

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CLIA Kit for Mouse VEGF-A

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U0143h

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96T

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CLIA Kit for Mouse VEGF-A

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U0143m

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96T

CLIA Kit for Mouse VEGF-A

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U0143c

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Polyclonal Antibody for Mouse VEGF-A

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P0143Rb-m

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40ug/0.2ml

Polyclonal Antibody for Mouse VEGF-A

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P0143Rb-h

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40ug/0.2ml

Polyclonal Antibody for Mouse VEGF-A

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P0143Rb-r

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Polyclonal Antibody for Mouse VEGF-A

Monoclonal Antibody for Mouse VEGF-A

Protein for Mouse VEGF-A

R&D Technical Data

12% SDS-PAGE Analysis

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 4.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 10.2%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤7.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤11.7%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of VEGF-A into serum and plasma .

Sample Type	Average(%)	Recovery Range (%)
Serum	86	80-95
Plasma	95	90-102

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of VEGFA into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	103	99-106
Plasma	102	96-107

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VEGF-A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	84-93%	79-94%	88-96%	78-91%
EDTA plasma(n=5)	78-89%	82-97%	90-102%	89-103%
heparin plasma(n=5)	83-94%	89-101%	87-99%	91-103%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VEGFA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	98-107%	101-104%	99-106%	100-106%
EDTA plasma(n=5)	100-105%	100-108%	100-105%	99-107%
heparin plasma(n=5)	97-109%	99-106%	95-110%	100-105%

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Citations

[1].

"Deneysel Staphylococcus aureus Yumuşak Doku Enfeksiyonlarında İbuprofenin Yara İyileşmesi Üzerindeki Etkisinin Araştırılması*"

M. Uğur ÇİTİL, E METE, E. Oğuzhan OĞUZ, et al.

From:Mikrobiyol Bul

References

1.

"Expression of vascular endothelial growth factor during embryonic angiogenesis and endothelial cell differentiation."
Breier G. , Albrecht U. , Sterrer S. , Risau W.
Development114:521-532(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS VEGF-1; VEGF-2 AND VEGF-3)
2.

"Vascular endothelial growth factor. Regulation by cell differentiation and activated second messenger pathways."
Claffey K.P. , Wilkison W.O. , Spiegelman B.M.
J. Biol. Chem.267:16317-16322(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM VEGF-1)
3.

"A novel alternatively spliced form of murine vascular endothelial growth factor, VEGF 115."
Sugihara T. , Wadhwa R. , Kaul S.C. , Mitsui Y.
J. Biol. Chem.273:3033-3038(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM VEGF-4)
strain: ICR.
4.

"Identification of a unique Mus musculus VEGF isoform of 102 amino acids."
Jankowsky J.A. , Adamski F.M. , Robertson F.M.
Submitted (2003-03) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM VEGF-5)
strain: FVB/N.
5.

"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M. , Goodstadt L. , Hillier L.W. , Zody M.C. , Goldstein S. , She X. , Bult C.J. , Agarwala R. , Cherry J.L. , DiCuccio M. , Hlavina W. , Kapustin Y. , Meric P. , Maglott D. , Birtle Z. , Marques A.C. , Graves T. , Zhou S. , more...
PLoS Biol.7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
strain: C57BL/6J.
6.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PARTIAL NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM L-VEGF-1)
strain: C57BL/6.
tissue: Brain.
7.

"Alternatively spliced forms of mouse VEGF."
Minchenko O.H. , Minchenko D.O. , Opentanova I.L.
Submitted (2004-09) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-123 (ISOFORMS VEGF-1/VEGF-2/VEGF-3)
strain: C57BL/6.
tissue: Retina.
8.

"The mouse gene for vascular endothelial growth factor. Genomic structure, definition of the transcriptional unit, and characterization of transcriptional and post-transcriptional regulatory sequences."
Shima D.T. , Kuroki M. , Deutsch U. , Ng Y. , Adamis A.P. , D'Amore P.A.
J. Biol. Chem.271:3877-3883(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-3 (ISOFORMS VEGF-1/VEGF-2/VEGF-3/VEGF-4/VEGF-5)
9.

"Regulation of VEGF and VEGF receptor expression in the rodent mammary gland during pregnancy, lactation, and involution."
Pepper M.S. , Baetens D. , Mandriota S.J. , Di Sanza C. , Oikemus S. , Lane T.F. , Soriano J.V. , Montesano R. , Iruela-Arispe M.L.
Dev. Dyn.218:507-524(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DEVELOPMENTAL STAGE
10.

"The regulators of VEGF expression in mouse ovaries."
Shin S.-Y. , Lee H.-J. , Ko D.-S. , Lee H.-C. , Park W.I.
Yonsei Med. J.46:679-686(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INDUCTION

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