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Index > Protein center > VEGFA(Gene name) > Pig
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  • VEGFA (Gene name),
  • Vascular endothelial growth factor A (Protein name ),  VEGFA_PIG from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Related Databases
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  • Citations  (1)
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  • Gene name:
    VEGFA(VEGF);
    Protein name:
    Vascular endothelial growth factor A(VEGF-A);
    Alternative:
    Vascular permeability factor(VPF);
    Organism:
    Pig (Sus scrofa). 
    General Annotation
    Sub Unit:
    Homodimer; disulfide-linked. Also found as heterodimer with PGF.
    Function:
    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin.
    Subcellular Location:
    Secreted Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.
    Protein Attributes:
    Sequence length:
    190
    Sequence:
    50:
    MNFLLSWVHW | SLALLLYLHH | AKWSQAAPMA | EGDQKPHEVV | KFMDVYQRSY | 
    100:
    CRPIETLVDI | FQEYPDEIEY | IFKPSCVPLM | RCGGCCNDEG | LECVPTEEFN | 
    150:
    ITMQIMRIKP | HQGQHIGEMS | FLQHNKCECR | PKKDRARQEN | PCGPCSERRK | 
    190:
    HLFVQDPQTC | KCSCKNTDSR | CKARQLELNE | RTCRCDKPRR | 
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    Pfam:
    UniGene:
    String:
    SMR:
    Uniprot:
     
    FOR
    ELISA Kit for Pig VEGF-A
    Cat.:
    E0143h
    Price:
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    MSDS:
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    Packing:
    96T
    Range:
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    ELISA Kit for Pig VEGF-A
    Cat.:
    E0143r
    Price:
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    MSDS:
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    Packing:
    96T
    Range:
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    ELISA Kit for Pig VEGF-A
    Cat.:
    E0143p
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    MSDS:
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    Packing:
    96T
    Range:
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    ELISA Kit for Pig VEGF-A
    Cat.:
    E0143c
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    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Pig VEGF-A
    Cat.:
    E0143d
    Price:
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    MSDS:
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    Packing:
    96T
    Range:
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    ELISA Kit for Pig VEGF-A
    Cat.:
    E0143m
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Pig VEGF-A
    Cat.:
    E0143b
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
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    CLIA Kit for Pig VEGF-A
    Cat.:
    U0143b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Pig VEGF-A
    Cat.:
    U0143d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Pig VEGF-A
    Cat.:
    U0143p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Pig VEGF-A
    Cat.:
    U0143r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Pig VEGF-A
    Cat.:
    U0143h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Pig VEGF-A
    Cat.:
    U0143m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Pig VEGF-A
    Cat.:
    U0143c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Pig VEGF-A
    Cat.:
    P0143Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Pig VEGF-A
    Cat.:
    P0143Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Pig VEGF-A
    Cat.:
    P0143Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Pig VEGF-A
    Polyclonal Antibody for Pig VEGF-A
    Polyclonal Antibody for Pig VEGF-A
    Polyclonal Antibody for Pig VEGF-A
    Monoclonal Antibody for Pig VEGF-A
    Monoclonal Antibody for Pig VEGF-A
    Monoclonal Antibody for Pig VEGF-A
    Monoclonal Antibody for Pig VEGF-A
    Monoclonal Antibody for Pig VEGF-A
    Monoclonal Antibody for Pig VEGF-A
    Monoclonal Antibody for Pig VEGF-A
    Protein for Pig VEGF-A
    Protein for Pig VEGF-A
    Protein for Pig VEGF-A
    Protein for Pig VEGF-A
    Protein for Pig VEGF-A
    Protein for Pig VEGF-A
    Protein for Pig VEGF-A

    R&D Technical Data

        12% SDS-PAGE Analysis

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    s

    s
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    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤ 4.8%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤ 10.2%

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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤7.5%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤11.7%

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    Recovery

    Recovery was determined by spiking various levels of VEGF-A into serum and plasma .

    Sample Type

    Average(%)

    Recovery  Range (%)

    Serum

    86

    80-95

    Plasma

    95

    90-102

     

     

     

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    Recovery was determined by spiking various levels of VEGFA into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    103

    99-106

    Plasma

    102

    96-107

     

     

     

     

    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    Linearity

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VEGF-A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    84-93%

    79-94%

    88-96%

    78-91%

    EDTA plasma(n=5)

    78-89%

    82-97%

    90-102%

    89-103%

    heparin plasma(n=5)

    83-94%

     

    89-101%

    87-99%

    91-103%

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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VEGFA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    98-107%

    101-104%

    99-106%

    100-106%

    EDTA plasma(n=5)

    100-105%

    100-108%

    100-105%

    99-107%

    heparin plasma(n=5)

    97-109%

     

    99-106%

    95-110%

    100-105%

     

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

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    Citations
    [1].
    References
    1. 1.
      "Nucleotide sequence and expression of the porcine vascular endothelial growth factor."
      Sharma H.S. , Tang Z.H. , Gho B.C.H. , Verdouw P.D.
      Biochim. Biophys. Acta1260:235-238(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      tissue: Heart.
    2. 2.
      "PCR cloning of porcine cardiac vascular endothelial growth factor gene."
      Lee T. , Canty J.M.
      Submitted (2000-11) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
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