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Index > Protein center > Vegfa(Gene name) > Rat
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  • Vegfa (Gene name),
  • Vascular endothelial growth factor A (Protein name ),  VEGFA_RAT from NCBI database.
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  • General Annotation
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  • Antigen Annotation
  • |
  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Related Databases
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  • Citations  (4)
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  • Gene name:
    Vegfa(Vegf);
    Protein name:
    Vascular endothelial growth factor A(VEGF-A);
    Alternative:
    Vascular permeability factor(VPF);
    Organism:
    Rat (Rattus norvegicus). 
    General Annotation
    Sub Unit:
    Homodimer; disulfide-linked. Also found as heterodimer with PGF.
    Function:
    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis.
    Subcellular Location:
    Secreted VEGF-A120 is acidic and freely secreted. VEGF-A164 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF-A188 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
    Protein Attributes:
    Sequence length:
    214
    Sequence:
    50:
    MNFLLSWVHW | TLALLLYLHH | AKWSQAAPTT | EGEQKAHEVV | KFMDVYQRSY | 
    100:
    CRPIETLVDI | FQEYPDEIEY | IFKPSCVPLM | RCAGCCNDEA | LECVPTSESN | 
    150:
    VTMQIMRIKP | HQSQHIGEMS | FLQHSRCECR | PKKDRTKPEK | KSVRGKGKGQ | 
    200:
    KRKRKKSRFK | SWSVHCEPCS | ERRKHLFVQD | PQTCKCSCKN | TDSRCKARQL | 
    214:
    ELNERTCRCD | KPRR
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    UniGene:
    String:
    KEGG:
    SMR:
    Pfam:
    Uniprot:
     
    FOR
    ELISA Kit for Rat VEGF-A
    Cat.:
    E0143h
    Price:
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    MSDS:
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    Packing:
    96T
    Range:
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    ELISA Kit for Rat VEGF-A
    Cat.:
    E0143r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
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    ELISA Kit for Rat VEGF-A
    Cat.:
    E0143p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
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    ELISA Kit for Rat VEGF-A
    Cat.:
    E0143c
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Rat VEGF-A
    Cat.:
    E0143d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Rat VEGF-A
    Cat.:
    E0143m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Rat VEGF-A
    Cat.:
    E0143b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat VEGF-A
    Cat.:
    U0143b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat VEGF-A
    Cat.:
    U0143d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat VEGF-A
    Cat.:
    U0143p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat VEGF-A
    Cat.:
    U0143r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat VEGF-A
    Cat.:
    U0143h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat VEGF-A
    Cat.:
    U0143m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat VEGF-A
    Cat.:
    U0143c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Rat VEGF-A
    Cat.:
    P0143Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat VEGF-A
    Cat.:
    P0143Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat VEGF-A
    Cat.:
    P0143Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat VEGF-A
    Polyclonal Antibody for Rat VEGF-A
    Polyclonal Antibody for Rat VEGF-A
    Polyclonal Antibody for Rat VEGF-A
    Monoclonal Antibody for Rat VEGF-A
    Monoclonal Antibody for Rat VEGF-A
    Monoclonal Antibody for Rat VEGF-A
    Monoclonal Antibody for Rat VEGF-A
    Monoclonal Antibody for Rat VEGF-A
    Monoclonal Antibody for Rat VEGF-A
    Monoclonal Antibody for Rat VEGF-A
    Protein for Rat VEGF-A
    Protein for Rat VEGF-A
    Protein for Rat VEGF-A
    Protein for Rat VEGF-A
    Protein for Rat VEGF-A
    Protein for Rat VEGF-A
    Protein for Rat VEGF-A

    R&D Technical Data

        12% SDS-PAGE Analysis

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    s

    s
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    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤ 4.8%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤ 10.2%

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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤7.5%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤11.7%

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    Recovery

    Recovery was determined by spiking various levels of VEGF-A into serum and plasma .

    Sample Type

    Average(%)

    Recovery  Range (%)

    Serum

    86

    80-95

    Plasma

    95

    90-102

     

     

     

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    Recovery was determined by spiking various levels of VEGFA into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    103

    99-106

    Plasma

    102

    96-107

     

     

     

     

    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    Linearity

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VEGF-A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    84-93%

    79-94%

    88-96%

    78-91%

    EDTA plasma(n=5)

    78-89%

    82-97%

    90-102%

    89-103%

    heparin plasma(n=5)

    83-94%

     

    89-101%

    87-99%

    91-103%

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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VEGFA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    98-107%

    101-104%

    99-106%

    100-106%

    EDTA plasma(n=5)

    100-105%

    100-108%

    100-105%

    99-107%

    heparin plasma(n=5)

    97-109%

     

    99-106%

    95-110%

    100-105%

     

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

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    References
    1. 1.
      "Amino acid and cDNA sequences of a vascular endothelial cell mitogen that is homologous to platelet-derived growth factor."
      Conn G. , Bayne M.L. , Soderman D.D. , Kwok P.W. , Sullivan K.A. , Palisi T.M. , Hope D.A. , Thomas K.A.
      Proc. Natl. Acad. Sci. U.S.A.87:2628-2633(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM VEGF-A164);PROTEIN SEQUENCE OF 27-190
    2. 2.
      "Developmental expression of vascular endothelial growth factor in the masseter muscle of rats."
      Ishii H. , Oota I. , Takuma T. , Inomata K.
      Arch. Oral Biol.46:77-82(2001) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS VEGF-A188; VEGF-A164; VEGF-A144 AND VEGF-A120)
    3. 3.
      "Cloning of multiple VEGF splice variants from hypoxic neonatal rat cardiomyocytes."
      Marion S. , Lee T.-C.
      Submitted (2001-04) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS VEGF-A164 AND VEGF-A188)
      strain: Sprague-Dawley.
    4. 4.
      "Purification and characterization of a naturally occurring vascular endothelial growth factor.placenta growth factor heterodimer."
      DiSalvo J. , Bayne M.L. , Conn G. , Kwok P.W. , Trivedi P.G. , Soderman D.D. , Palisi T.M. , Sullivan K.A. , Thomas K.A.
      J. Biol. Chem.270:7717-7723(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 27-40;HETERODIMERIZATION WITH PGF
      tissue: Glial tumor.
    5. 5.
      "Regulation of VEGF and VEGF receptor expression in the rodent mammary gland during pregnancy, lactation, and involution."
      Pepper M.S. , Baetens D. , Mandriota S.J. , Di Sanza C. , Oikemus S. , Lane T.F. , Soriano J.V. , Montesano R. , Iruela-Arispe M.L.
      Dev. Dyn.218:507-524(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;TISSUE SPECIFICITY;DEVELOPMENTAL STAGE
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