Language:
  
[Sign in] [Register]   

EIAab logo

EIAab news detail, please contact eiaab@eiaab.com if you have any questions about online orders and payment.
Index > >
The method for drawing Standard curve
Update time:2014-09-25 21:06:07   【 Font: Large  Medium Small

Curveexpert: Curve expert

 

Normally people draw ELISA standard curve by using specialized tools for curve fitting, such as: Curve Expert 1.3
Below is "Curve Exert1.3" software, for example, draw the ELISA standard curve as follows:
1. Start "Curve Expert1.3" (Curve Expert1.3 free download. Zip)
2. X-axis: input values corresponding to the concentration, Y-axis: input the OD values of standard, as shown:
 
 
3.Click [Run] button, the following dialog box appears
 
 
4 Click [ok] button, the following two dialog boxes appear, then close the dialog box below
 
 
5. A list of some curves appears at the upper right corner of the dialog box, from "1" Start to click the name of curves, then the corresponding fitting curve will appear in the lower right corner.

6. Double click the best fitting curve for ELISA test, the dialog box appears as follows:
Note: select curve equation with the best coefficient (the "r" value) for computing. In the following dialog box, the "r" value at the upper right corner (close to 1 means the better fitting)
 
 
 
7.  Press [Ctrl] key + [L] key, the following dialog box appears:
 
 
 
 
8. Enter the standard OD values, click [Calculate] button, you can get the actual content of the target protein. (N-fold diluted samples, calculate the value should be multiplied by N).
 
9. If you would like to have the curve fitting equation of ELISA, right-click blank space of the dialog box in step 6, choose "Information"
 
10.Get the following dialog box: Click "Copy"
 
 
 
You can paste in the location you want to obtain the data as follows:
 
3rd degree Polynomial Fit:  y=a+bx+cx^2+dx^3...
Coefficient Data:
a =   -0.0010925264
b =  0.11260362
c =   0.00071609266
d =  -4.6172104e-005
 
 
 
 
by EIAab organize the information.
Hot Genes
ALCAM ACE KSR2 ASPRO C19orf80 Gdf5 Trap1a Atf2
Top Searches
Asprosin Ubiquitin ELISA Ubiquitin-protein ligase TRAP1A metalloproteinase Tumor necrosis vitamin d
Why choose EIAAB
Our products have been quoted by many publications in famous journals such as Cell; Cell Metabolism; Hepatology; Biomaterials.more
Further Information
About us Protein center Bank account Distributors Terms & Conditions Career eiaab.cn

Copyright & copy www.eiaab.com2006-2016 All Rights Reserved    EIAab         Email:eiaab@eiaab.com

Twitter