Angiogenin is the first cytokine identified to have a pro-Angiogenic effect from human tumor tissue. The family consists of four members, Ang1、2、3、4. Ang1 is synthesized by pericytes, vascular smooth muscle cells and tumor cells. Under the action of paracrine, Ang1 binds to receptors on adjacent endothelial cell membranes, phosphorylates the receptors, and then initiates signal transmission. But the exact process remains unclear. It is clear that Ang1 plays an important role in inhibiting endothelial cell apoptosis, reducing vascular atrophy and degrading cell life activities. According to the mechanism of Ang1, the researchers took
Ang1 is associated with tumor development. Studies have shown that Ang1 can promote tumor angiogenesis. Although the mechanism is not clear, it is an indisputable fact that Ang1 is involved in tumor angiogenesis. The possible mechanism of Ang1 in tumor angiogenesis is inhibition of endothelial cell apoptosis. The anti - apoptosis effect of Ang1 is a series of complex biochemical reactions. After Ang1 binds to the receptor, the subunit of PI3K linked to the receptor is phosphorylated to activate PI3K. PI3K ACTS on inositol phosphate to increase the contents of inositol 1, 4 and 5 triphosphate and inositol 3, 4 and 5 triphosphate, which positively regulate Ser/Thr kinase. Phosphorylation sites are Thr308 and Ser473. Survivin expression after Ang1 inhibits the phosphorylation of caspase7 and caspase9. Therefore, Ang1 may promote tumor angiogenesis in the process of tumor development, but this mechanism still needs to be further studied.
E0008m is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Angiopoietin-1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Angiopoietin-1. The ELISA analytical biochemical technique of the E0008m kit is based on Angiopoietin-1 antibody-Angiopoietin-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Angiopoietin-1 antigen targets in samples
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