[Sign in] [Register]   

EIAab logo

EIAab news detail, please contact if you have any questions about online orders and payment.
Carvedilol protects against hepatic ischemia reperfusion injury in high-fructose high-fat diet-fed mice: Role of G protein-coupled receptor kinase 2 a
Update time:2019-09-28 23:19:00   【 Font: Large  Medium Small


Hepatic ischemia/reperfusion injury (H-IRI) is associated with irreversible liver damage. The current study aimed to investigate the protective effect of carvedilol against H-IRI in high fructose high fat diet (HFrHFD) fed mice and the role of G-protein coupled receptor kinase 2 and 5 (GRK2 and GRK5). Mice were fed HFrHFD for 16?weeks, at the end of feeding period, mice were subjected to 30?min of ischemia followed by 1?h of reperfusion. Carvedilol (20?mg/kg, i.p.) was administered 30?min before ischemia. To explore the role of GRK2 and GRK5 in mediating carvedilol effects, paroxetine (GRK2 inhibitor, 10?mg/kg, i.p.) and amlexanox (GRK5 inhibitor, 25?mg/kg, i.p.) were administered 30?min before carvedilol administration. Liver function, histopathology and hepatic oxidative stress, as well as inflammatory and apoptotic markers were measured at the end of the experiment. In addition, adrenergic receptors downstream signals were measured in the liver. Results showed increased markers of liver injury (ALT and AST) in mice subjected to H-IRI. Moreover, liver injury was associated with slight collagen deposits as revealed by histopathology and elevated hepatic levels of oxidative stress, inflammatory and apoptotic markers. On the other hand, carvedilol protected mice against H-IRI and improved all associated pathological changes. Furthermore, pre-injection of either GRK2i or GRK5i did not change carvedilol effects on serum ALT level and liver collagen deposits, while increased its antioxidant, anti-inflammatory and anti-apoptotic effects. In conclusion, carvedilol protects against H-IRI in HFrHFD-fed mice. GRK2 and GRK5 may not play a potential role in mediating this effect.

Cited products
Source:Toxicology and Applied Pharmacology      by S G. Mohammed, A A.A.Mahmoud, M F. Mahmoud, et al.
Hot Genes
Atf2 ASPRO ACE ALCAM C19orf80 Trap1a KSR2 Gdf5
Top Searches
Ubiquitin-protein ligase Ubiquitin ELISA metalloproteinase Tumor necrosis Asprosin TRAP1A Alpha
Why choose EIAAB
Our products have been quoted by many publications in famous journals such as Cell; Cell Metabolism; Hepatology; Biomaterials.more
Further Information
About us Protein center Bank account Distributors Terms & Conditions Career

Copyright & copy www.eiaab.com2006-2016 All Rights Reserved    EIAab