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A novel system of artificial antigen-presenting cells efficiently stimulates Flu peptide-specific cytotoxic T cells in vitro
Update time:2012-02-28 16:08:46   【 Font: Large  Medium Small

Abstract

adoptive immunotherapy. However, efficient and reproducible methods to meet the qualification remain poor. To address this issue, we designed the artificial antigen-presenting cell (aAPC) system based on poly(lactic-co-glycolic acid) (PLGA). A modified emulsion method was used for the preparation of PLGA particles encapsulating interleukin-2 (IL-2). Biotinylated molecular ligands for recognition and co-stim- ulation of T cells were attached to the particle surface through the binding of avidin–biotin. These formed the aAPC system. The function of aAPCs in the proliferation of specific CTLs against human Flu antigen Q1 was detected by enzyme-linked immunospot assay (ELISA) and MTT staining methods. Finally, we suc-
cessfully prepared this suitable aAPC system. The results show that IL-2 is released from aAPCs in a sus- tained manner over 30 days. This dramatically improves the stimulatory capacity of this system as compared to the effect of exogenous addition of cytokine. In addition, our aAPCs promote the prolifera- tion of Flu antigen-specific CTLs more effectively than the autologous cellular APCs. Here, this aAPC plat- form is proved to be suitable for expansion of human antigen-specific T cells.

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Source:Biochemical and Biophysical Research Communications      by Hui Han a,1,2, Ji-Run Peng a,.,2, Peng-Cheng Chen a, Lei Gong a, Shi-Shi Qiao b, Wen-Zhen Wang a,Zhu-Qingqing Cui a, Xin Yu a, Yu-Hua Wei a, Xi-Sheng Leng a,?
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