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Index > Protein center > ACE(Gene name) > Rabbit
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  • ACE (Gene name),
  • Angiotensin-converting enzyme (Protein name ),  ACE_RABIT from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Gene name:
    ACE(DCP1);
    Protein name:
    Angiotensin-converting enzyme(ACE);
    Alternative:
    Dipeptidyl carboxypeptidase I;Kininase II;
    CD:
    CD143;
    Organism:
    Rabbit (Oryctolagus cuniculus). 
    General Annotation
    Sub Unit:
    N/A
    Function:
    Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.
    Subcellular Location:
    Cell membrane Single-pass type I membrane protein
    Protein Attributes:
    Sequence length:
    1310
    Sequence:
    50:
    MGAAPGRRGP | RLLRPPPPLL | LLLLLLRPPP | AALTLDPGLL | PGDFAADEAG | 
    100:
    ARLFASSYNS | SAEQVLFRST | AASWAHDTNI | TAENARRQEE | EALLSQEFAE | 
    150:
    AWGKKAKELY | DPVWQNFTDP | ELRRIIGAVR | TLGPANLPLA | KRQQYNSLLS | 
    200:
    NMSQIYSTGK | VCFPNKTASC | WSLDPDLNNI | LASSRSYAML | LFAWEGWHNA | 
    250:
    VGIPLKPLYQ | EFTALSNEAY | RQDGFSDTGA | YWRSWYDSPT | FEEDLERIYH | 
    300:
    QLEPLYLNLH | AYVRRVLHRR | YGDRYINLRG | PIPAHLLGNM | WAQSWESIYD | 
    350:
    MVVPFPDKPN | LDVTSTMVQK | GWNATHMFRV | AEEFFTSLGL | LPMPPEFWAE | 
    400:
    SMLEKPEDGR | EVVCHASAWD | FYNRKDFRIK | QCTQVTMDQL | STVHHEMGHV | 
    450:
    QYYLQYKDQP | VSLRRANPGF | HEAIGDVLAL | SVSTPAHLHK | IGLLDHVTND | 
    500:
    TESDINYLLK | MALEKIAFLP | FGYLVDQWRW | GVFSGRTPSS | RYNFDWWYLR | 
    550:
    TKYQGICPPV | VRNETHFDAG | AKFHIPSVTP | YIRYFVSFVL | QFQFHQALCM | 
    600:
    EAGHQGPLHQ | CDIYQSTRAG | AKLRAVLQAG | CSRPWQEVLK | DMVASDALDA | 
    650:
    QPLLDYFQPV | TQWLQEQNER | NGEVLGWPEY | QWRPPLPNNY | PEGIDLVTDE | 
    700:
    AEASRFVEEY | DRSFQAVWNE | YAEANWNYNT | NITTEASKIL | LQKNMQIANH | 
    750:
    TLTYGNWARR | FDVSNFQNAT | SKRIIKKVQD | LQRAVLPVKE | LEEYNQILLD | 
    800:
    METIYSVANV | CRVDGSCLQL | EPDLTNLMAT | SRKYDELLWV | WTSWRDKVGR | 
    850:
    AILPYFPKYV | EFTNKAARLN | GYVDAGDSWR | SMYETPTLEQ | DLERLFQELQ | 
    900:
    PLYLNLHAYV | GRALHRHYGA | QHINLEGPIP | AHLLGNMWAQ | TWSNIYDLVA | 
    950:
    PFPSASTMDA | TEAMIKQGWT | PRRMFEEADK | FFISLGLLPV | PPEFWNKSML | 
    1000:
    EKPTDGREVV | CHASAWDFYN | GKDFRIKQCT | TVNMEDLVVV | HHEMGHIQYF | 
    1050:
    MQYKDLPVAL | REGANPGFHE | AIGDVLALSV | STPKHLHSIN | LLSSEGGGYE | 
    1100:
    HDINFLMKMA | LDKIAFIPFS | YLVDEWRWRV | FDGSITKENY | NQEWWSLRLK | 
    1150:
    YQGLCPPAPR | SQGDFDPGAK | FHIPSSVPYI | RYFVSFIIQF | QFHEALCKAA | 
    1200:
    GHTGPLHTCD | IYQSKEAGKR | LADAMKLGYS | KPWPEAMKVI | TGQPNMSASA | 
    1250:
    MMNYFKPLMD | WLLTENGRHG | EKLGWPQYTW | TPNSARSEGS | LPDSGRVNFL | 
    1300:
    GMNLDAQQAR | VGQWVLLFLG | VALLLASLGL | TQRLFSIRYQ | SLRQPHHGPQ | 
    1310:
    FGSEVELRHS | 
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
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    Related Databases
    SMR:
    String:
    UniGene:
    Pfam:
    Uniprot:
     
    FOR
    ELISA Kit for Rabbit ACE
    Cat.:
    E0004h
    Price:
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    MSDS:
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    Packing:
    96T
    Range:
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    ELISA Kit for Rabbit ACE
    Cat.:
    E0004m
    Price:
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    MSDS:
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    Packing:
    96T
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    ELISA Kit for Rabbit ACE
    Cat.:
    E0004r
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    MSDS:
    Please sign in first.
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    96T
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    ELISA Kit for Rabbit ACE
    Cat.:
    E0004b
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    MSDS:
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    Packing:
    96T
    Range:
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    ELISA Kit for Rabbit ACE
    MSDS:
    Please sign in first.
    ELISA Kit for Rabbit ACE
    Cat.:
    E0004c
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    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rabbit ACE
    MSDS:
    Please sign in first.
    CLIA Kit for Rabbit ACE
    Cat.:
    U0004h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rabbit ACE
    Cat.:
    U0004m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rabbit ACE
    Cat.:
    U0004r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rabbit ACE
    Cat.:
    U0004c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rabbit ACE
    Cat.:
    U0004b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Rabbit ACE
    Polyclonal Antibody for Rabbit ACE
    Cat.:
    P0004Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rabbit ACE
    Polyclonal Antibody for Rabbit ACE
    Cat.:
    P0004Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rabbit ACE
    Cat.:
    P0004Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rabbit ACE
    Monoclonal Antibody for Rabbit ACE
    Monoclonal Antibody for Rabbit ACE
    Monoclonal Antibody for Rabbit ACE
    Monoclonal Antibody for Rabbit ACE
    Monoclonal Antibody for Rabbit ACE
    Monoclonal Antibody for Rabbit ACE
    Protein for Rabbit ACE
    Protein for Rabbit ACE
    Protein for Rabbit ACE
    Protein for Rabbit ACE
    Protein for Rabbit ACE
    Protein for Rabbit ACE

    R&D Technical Data
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    s

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    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤4.0%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.9%



     

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    Recovery

    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery  Range (%)

    Serum

    101

    95-107

    Plasma

    99

    93-102

     

     

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    Linearity

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    Saliva(n=3)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    93-104%

    91-101%

    95-102%

    96-109%

    Saliva(n=3)

    90-99%

    93- 99%

    89-97%

    92-106%

    heparin plasma(n=5)

    94-103%

     

    96-110%

    99-111%

    94-110%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    References
    1. 1.
      "Use of alternative polyadenylation sites for tissue-specific transcription of two angiotensin-converting enzyme mRNAs."
      Thekkumkara T.J. , Livingston W. III , Kumar R.S. , Sen G.C.
      Nucleic Acids Res.20:683-687(1992) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SOMATIC)
      tissue: Lung.
    2. 2.
      Sen G.C.
      Submitted (1997-03) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SEQUENCE REVISION
    3. 3.
      "Structure of testicular angiotensin-converting enzyme. A segmental mosaic isozyme."
      Kumar R.S. , Kusari J. , Roy S.N. , Soffer R.L. , Sen G.C.
      J. Biol. Chem.264:16754-16758(1989) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM TESTIS-SPECIFIC)
      strain: New Zealand white.
      tissue: Testis.
    4. 4.
      "The mRNAs encoding the two angiotensin-converting isozymes are transcribed from the same gene by a tissue-specific choice of alternative transcription initiation sites."
      Kumar R.S. , Thekkumkara T.J. , Sen G.C.
      J. Biol. Chem.266:3854-3862(1991) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-88;ALTERNATIVE SPLICING
      tissue: Liver.
    5. 5.
      "The NH2- and COOH-terminal sequences of the angiotensin-converting enzyme isozymes from rabbit lung and testis."
      Iwata K. , Lai C.Y. , El-Dorry H.A. , Soffer R.L.
      Biochem. Biophys. Res. Commun.107:1097-1103(1982) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 34-44 AND 755-758
    6. 6.
      "Rabbit pulmonary angiotensin-converting enzyme: the NH2-terminal fragment with enzymatic activity and its formation from the native enzyme by NH4OH treatment."
      Iwata K. , Blacher R. , Soffer R.L. , Lai C.Y.
      Arch. Biochem. Biophys.227:188-201(1983) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 34-55
      tissue: Lung.
    7. 7.
      "The Mg(2+)-ATPase of rabbit skeletal-muscle transverse tubule is a highly glycosylated multiple-subunit enzyme."
      Kirley T.L.
      Biochem. J.278:375-380(1991) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 34-55;GLYCOSYLATION
    8. 8.
      "Angiotensin-converting enzyme: structural relationship of the testicular and the pulmonary forms."
      Sen G.C. , Thekkumkara T.J. , Kumar R.S.
      J. Cardiovasc. Pharmacol.16:S14-S18(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE OF 646-746
    9. 9.
      "Identification of essential tyrosine and lysine residues in angiotensin converting enzyme: evidence for a single active site."
      Chen Y.N. , Riordan J.F.
      Biochemistry29:10493-10498(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 727-733 AND 809-815
    10. 10.
      "Regulated cleavage-secretion of the membrane-bound angiotensin-converting enzyme."
      Ramchandran R. , Sen G.C. , Misono K. , Sen I.
      J. Biol. Chem.269:2125-2130(1994) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 1237-1259;CLEAVAGE SITE
    11. 11.
      "Mutations in two specific residues of testicular angiotensin-converting enzyme change its catalytic properties."
      Sen I. , Kasturi S. , Abdul-Jabbar M. , Sen G.C.
      J. Biol. Chem.268:25748-25754(1993) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MUTAGENESIS OF LYS-727 AND TYR-809;ENZYME REGULATION;BIOPHYSICOCHEMICAL PROPERTIES
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