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12% SDS-PAGE Analysis
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Precision
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤ 4.5%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤ 7.1%
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Recovery
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Recovery was determined by spiking various levels of ACE into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
95
91-98
Plasma
97
92-102
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Linearity
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1：2
1:4
1:8
1:16
serum(n=5)
87-95%
90-101%
92-103%
90-98%
EDTA plasma(n=5)
89-95%
91-102%
89-101%
90-102%
heparin plasma(n=5)
90-98%
92-105%
96-101%
89-99%
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Vaild Sequence:
