Angiogenin (Protein name
), ANGI_HUMAN from NCBI database.
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General Annotation
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Antigen Annotation
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3D
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Predicted Eptitope
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Vaild Sequence
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Gene name:
ANG(RNASE5);
Protein name:
Angiogenin;
Alternative:
Ribonuclease 5(RNase 5);
Organism:
Human (Homo sapiens).
General Annotation
Sub Unit:
Interacts with and forms a tight 1:1 complex with RNH1. Dimerization of two such complexes may occur.
Function:
May function as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo.
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤ 5.9%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤ 9.2%
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Recovery
Recovery was determined by spiking various levels of ACE into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
95
91-101
Plasma
94
90-99
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Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
89-99%
91-102%
93-103%
90-104%
EDTA plasma(n=5)
89-98%
90-103%
91-97%
92-102%
heparin plasma(n=5)
90-101%
92-105%
94-107%
91-106%
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Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANT GLU-84
3.
Li J.
,
Wang H.
Submitted (2008-09) to the EMBL/GenBank/DDBJ databases
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Cited for: NUCLEOTIDE SEQUENCE [MRNA]
4.
"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L.
,
Schick M.
,
Neubert P.
,
Schatten R.
,
Henze S.
,
Korn B.
Submitted (2004-05) to the EMBL/GenBank/DDBJ databases
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Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
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Cited for: PROTEIN SEQUENCE OF 25-147;PYROGLUTAMATE FORMATION AT GLN-25;DISULFIDE BONDS
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Cited for: SUBCELLULAR LOCATION;NUCLEOLAR LOCALIZATION SIGNAL
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Cited for: MUTAGENESIS OF HIS-32; GLN-36; LYS-64; ASN-92 AND GLU-132
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Cited for: INTERACTION WITH RNH1;MUTAGENESIS OF ARG-29 AND LYS-64
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Cited for: FUNCTION;INTERACTION WITH RNH1;MUTAGENESIS OF 109-GLY-GLY-110
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Cited for: X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 25-147
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Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 25-147 IN COMPLEX WITH RNH1 AND SUBUNIT
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Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 26-147
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 26-147 OF MUTANT GLY-141 IN COMPLEX WITH PHOSPHATE AND PYROPHOSPHATE
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Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 26-147;MUTAGENESIS OF ASP-140; GLN-141 AND 143-ILE-PHE-144
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Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 26-147
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Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF MUTANTS ASP-68 AND ALA-104
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Cited for: VARIANTS ALS9 LEU-36; ILE-41; GLU-41; LYS-55; TRP-63 AND ILE-64;VARIANT VAL-70
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Cited for: VARIANTS ALS9 SER-20; ILE-41; ASN-52 AND LEU-136;CHARACTERIZATION OF VARIANTS ALS9 ILE-41; ASN-52 AND LEU-136;TISSUE SPECIFICITY
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Cited for: CHARACTERIZATION OF VARIANTS ALS9 LEU-36; ILE-41; GLU-41; LYS-55; TRP-63 AND ILE-64;CHARACTERIZATION OF VARIANT VAL-70
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Cited for: VARIANTS ALS9 SER-12; SER-20; ILE-137 AND ARG-138;VARIANT VAL-70