Ccl2 ELISA Kit for Rat(Rattus norvegicus) | EIAab.comCcl2 ELISA Kit for Rat(Rattus norvegicus)

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Index > Protein center > Ccl2(Gene name) > Rat

Ccl2 (Gene name),

C-C motif chemokine 2 (Protein name ), CCL2_RAT from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

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Gene name:

Ccl2(Je;Mcp1;Scya2);

Protein name:

C-C motif chemokine 2;

Alternative:

Monocyte chemoattractant protein 1;Immediate-early serum-responsive protein JE;Small-inducible cytokine A2;Monocyte chemotactic protein 1(MCP-1);

Organism:

Rat (Rattus norvegicus).

General Annotation

Sub Unit:

Monomer or homodimer; in equilibrium.

Function:

Chemotactic factor that attracts monocytes, but not neutrophils.

Subcellular Location:

Secreted

Protein Attributes:

Sequence length:

148

Sequence:

50:

100:

148:

VFYKIASTLR | TSAPLNVNLT | HKSEANASTL | FSTTTSSTSV | EVTSMTEN

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

STRING:

10116.ENSRNOP00000009448

Pfam:

PF00048

UniGene:

Rn.4772

String:

P14844

KEGG:

rno:24770

Uniprot:

P14844

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ELISA Kit for Rat C-C motif chemokine 2

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ELISA Kit for Rat C-C motif chemokine 2

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E0087h

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ELISA Kit for Rat C-C motif chemokine 2

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E0087m

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ELISA Kit for Rat C-C motif chemokine 2

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E0087b

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ELISA Kit for Rat C-C motif chemokine 2

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E0087r

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ELISA Kit for Rat C-C motif chemokine 2

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ELISA Kit for Rat C-C motif chemokine 2

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E0087d

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CLIA Kit for Rat C-C motif chemokine 2

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U0087p

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CLIA Kit for Rat C-C motif chemokine 2

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U0087r

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CLIA Kit for Rat C-C motif chemokine 2

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U0087d

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CLIA Kit for Rat C-C motif chemokine 2

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U0087b

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CLIA Kit for Rat C-C motif chemokine 2

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U0087m

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Looking for manual.

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CLIA Kit for Rat C-C motif chemokine 2

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U0087h

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CLIA Kit for Rat C-C motif chemokine 2

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Polyclonal Antibody for Rat C-C motif chemokine 2

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P0087Rb-r

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40ug/0.2ml

Polyclonal Antibody for Rat C-C motif chemokine 2

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P0087Rb-m

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Polyclonal Antibody for Rat C-C motif chemokine 2

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P0087Rb-h

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Monoclonal Antibody for Rat C-C motif chemokine 2

Protein for Rat C-C motif chemokine 2

Cat.:

R0087b

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Packing:

1mg

Purity:

>90%

Protein for Rat C-C motif chemokine 2

Cat.:

R0087d

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1mg

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>90%

Protein for Rat C-C motif chemokine 2

Cat.:

R0087p

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1mg

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>90%

Protein for Rat C-C motif chemokine 2

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R0087h

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Packing:

1mg

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>90%

Protein for Rat C-C motif chemokine 2

R&D Technical Data

12% SDS-PAGE analysis

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of CCL2 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	91-99
Plasma	96	94-103

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CCL2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-98%	96-103%	97-105%	102-108%
EDTA plasma(n=5)	96-101%	98-105%	99-106%	91-96%
heparin plasma(n=5)	90-103%	91-104%	94-105%	95-102%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Analysis of the rat JE gene promoter identifies an AP-1 binding site essential for basal expression but not for TPA induction."
Timmers H.T.M. , Pronk G.J. , Bos J.L. , van der Eb A.J.
Nucleic Acids Res.18:23-34(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
strain: WAG/RIJ.
tissue: Kidney.
2.

"Molecular cloning of rat monocyte chemoattractant protein-1 (MCP-1) and its expression in rat spleen cells and tumor cell lines."
Yoshimura T. , Takeya M. , Takahashi K.
Biochem. Biophys. Res. Commun.174:504-509(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
3.

"Cloning of the full-length rat JE/MCP-1 cDNA."
Poon M. , Taubman M.B.
Submitted (1998-04) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
strain: Sprague-Dawley.
tissue: Aortic smooth muscle.

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