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Index > Protein center > CYCS(Gene name) > Human
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  • CYCS (Gene name),
  • Cytochrome c (Protein name ),  CYC_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Related Databases
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  • Citations  (1)
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  • Gene name:
    CYCS(CYC);
    Protein name:
    Cytochrome c;
    Alternative:

    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    N/A
    Function:
    Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
    Subcellular Location:
    Mitochondrion matrix
    Protein Attributes:
    Sequence length:
    105
    Sequence:
    50:
    MGDVEKGKKI | FIMKCSQCHT | VEKGGKHKTG | PNLHGLFGRK | TGQAPGYSYT | 
    100:
    AANKNKGIIW | GEDTLMEYLE | NPKKYIPGTK | MIFVGIKKKE | ERADLIAYLK | 
    105:
    KATNE
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    SMR:
    MIM:
    UniGene:
    KEGG:
    Pfam:
    String:
    Uniprot:
     
    FOR
    ELISA Kit for Human Cytochrome c
    Cat.:
    E0594r
    Price:
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    MSDS:
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    Packing:
    96T
    Range:
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    ELISA Kit for Human Cytochrome c
    Cat.:
    E0594h
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
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    ELISA Kit for Human Cytochrome c
    Cat.:
    E0594m
    Price:
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    MSDS:
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    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Cytochrome c
    Cat.:
    E0594d
    Price:
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    MSDS:
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    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Cytochrome c
    Cat.:
    E0594b
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Cytochrome c
    Cat.:
    E0594p
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
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    ELISA Kit for Human Cytochrome c
    MSDS:
    Please sign in first.
    CLIA Kit for Human Cytochrome c
    Cat.:
    U0594m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Cytochrome c
    Cat.:
    U0594d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Cytochrome c
    MSDS:
    Please sign in first.
    CLIA Kit for Human Cytochrome c
    Cat.:
    U0594b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Cytochrome c
    Cat.:
    U0594r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Cytochrome c
    Cat.:
    U0594p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Cytochrome c
    Cat.:
    U0594h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
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    Polyclonal Antibody for Human Cytochrome c
    Cat.:
    P0594Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Cytochrome c
    Polyclonal Antibody for Human Cytochrome c
    Polyclonal Antibody for Human Cytochrome c
    Polyclonal Antibody for Human Cytochrome c
    Polyclonal Antibody for Human Cytochrome c
    Cat.:
    P0594Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Cytochrome c
    Cat.:
    P0594Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Human Cytochrome c
    Monoclonal Antibody for Human Cytochrome c
    Monoclonal Antibody for Human Cytochrome c
    Monoclonal Antibody for Human Cytochrome c
    Monoclonal Antibody for Human Cytochrome c
    Monoclonal Antibody for Human Cytochrome c
    Monoclonal Antibody for Human Cytochrome c
    Protein for Human Cytochrome c
    Protein for Human Cytochrome c
    Protein for Human Cytochrome c
    Protein for Human Cytochrome c
    Protein for Human Cytochrome c
    Protein for Human Cytochrome c
    Protein for Human Cytochrome c

    R&D Technical Data
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    s

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    s
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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    Recovery
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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

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    References
    1. 1.
      "The human somatic cytochrome c gene: two classes of processed pseudogenes demarcate a period of rapid molecular evolution."
      Evans M.J. , Scarpulla R.C.
      Proc. Natl. Acad. Sci. U.S.A.85:9625-9629(1988) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    2. 2.
      "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N. , Chen X. , Rolfs A. , Halleck A. , Hines L. , Eisenstein S. , Koundinya M. , Raphael J. , Moreira D. , Kelley T. , LaBaer J. , Lin Y. , Phelan M. , Farmer A.
      Submitted (2003-05) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
    3. 3.
      "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
      Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Cerebellum.
    4. 4.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Amygdala.
    5. 5.
      "The DNA sequence of human chromosome 7."
      Hillier L.W. , Fulton R.S. , Fulton L.A. , Graves T.A. , Pepin K.H. , Wagner-McPherson C. , Layman D. , Maas J. , Jaeger S. , Walker R. , Wylie K. , Sekhon M. , Becker M.C. , O'Laughlin M.D. , Schaller M.E. , Fewell G.A. , Delehaunty K.D. , Miner T.L. , more...
      Nature424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    6. 7.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    7. 8.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Bone marrow.
      tissue: Brain.
      tissue: Kidney.
      tissue: Lung.
      tissue: Skeletal muscle.
      tissue: Skin.
      tissue: Testis.
      tissue: Urinary bladder.
    8. 9.
      "The amino acid sequence of human heart cytochrome c."
      Matsubara H. , Smith E.L.
      J. Biol. Chem.237:3575-3576(1962) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 2-105;ACETYLATION AT GLY-2
      tissue: Heart.
    9. 10.
      "Human heart cytochrome c. Chymotryptic peptides, tryptic peptides, and the complete amino acid sequence."
      Matsubara H. , Smith E.L.
      J. Biol. Chem.238:2732-2753(1963) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 2-105
      tissue: Heart.
    10. 11.
      "Cytochrome c in the apoptotic and antioxidant cascades."
      Skulachev V.P.
      FEBS Lett.423:275-280(1998) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW ON ROLE IN APOPTOSIS
    11. 12.
      "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
      Choudhary C. , Kumar C. , Gnad F. , Nielsen M.L. , Rehman M. , Walther T.C. , Olsen J.V. , Mann M.
      Science325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
    12. 13.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
    13. 14.
      "Solution structure of reduced recombinant human cytochrome c."
      Jeng W.-Y. , Shiu J.-H. , Tsai Y.-H. , Chuang W.-J.
      Submitted (2003-02) to the PDB data bank
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR
    14. 15.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT THC4 SER-42;IDENTIFICATION BY MASS SPECTROMETRY;CHARACTERIZATION OF VARIANT THC4 SER-42;X-RAY CRYSTALLOGRAPHY (2.75 ANGSTROMS) OF VARIANT THC4 SER-42 AND WILD TYPE
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