Epo ELISA Kit for Rat(Rattus norvegicus) | EIAab.comEpo ELISA Kit for Rat(Rattus norvegicus)

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Index > Protein center > Epo(Gene name) > Rat

Epo (Gene name),

Erythropoietin (Protein name ), EPO_RAT from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

Citations (4)

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Gene name:

Epo;

Protein name:

Erythropoietin;

Alternative:

Organism:

Rat (Rattus norvegicus).

General Annotation

Sub Unit:

N/A

Function:

Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.

Subcellular Location:

Secreted

Protein Attributes:

Sequence length:

192

Sequence:

50:

100:

150:

192:

TQAAPLRTLT | ADTFCKLFRV | YSNFLRGKLK | LYTGEACRRG | DR

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

UniGene:

Rn.11365

Pfam:

PF00758

String:

P29676

STRING:

10116.ENSRNOP00000001914

SMR:

P29676

KEGG:

rno:24335

Uniprot:

P29676

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ELISA Kit for Rat Erythropoietin

Cat.:

E0028r

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96T

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ELISA Kit for Rat Erythropoietin

Cat.:

E0028h

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96T

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ELISA Kit for Rat Erythropoietin

Cat.:

E0028d

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96T

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ELISA Kit for Rat Erythropoietin

Cat.:

E0028p

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96T

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ELISA Kit for Rat Erythropoietin

Cat.:

E0028m

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96T

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ELISA Kit for Rat Erythropoietin

Cat.:

E0028b

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96T

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ELISA Kit for Rat Erythropoietin

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CLIA Kit for Rat Erythropoietin

Cat.:

U0028b

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Packing:

96T

CLIA Kit for Rat Erythropoietin

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CLIA Kit for Rat Erythropoietin

Cat.:

U0028m

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Looking for manual.

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96T

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CLIA Kit for Rat Erythropoietin

Cat.:

U0028h

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Looking for manual.

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96T

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CLIA Kit for Rat Erythropoietin

Cat.:

U0028d

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Looking for manual.

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96T

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CLIA Kit for Rat Erythropoietin

Cat.:

U0028p

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Looking for manual.

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96T

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CLIA Kit for Rat Erythropoietin

Cat.:

U0028r

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Looking for manual.

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96T

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Polyclonal Antibody for Rat Erythropoietin

Cat.:

P0028Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Rat Erythropoietin

Cat.:

P0028Rb-r

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40ug/0.2ml

Polyclonal Antibody for Rat Erythropoietin

Cat.:

P0028Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Rat Erythropoietin

Monoclonal Antibody for Rat Erythropoietin

Protein for Rat Erythropoietin

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.1%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of EPO into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	96	94-99
Plasma	98	97-102

Recovery was determined by spiking various levels of EPO into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	91-103
Plasma	98	93-106

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of EPO into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	95-103
Plasma	98	97-105

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EPO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-100%	90-98%	95-102%	92-97%
EDTA plasma(n=5)	89-95%	93-98%	99-105%	90-97%
heparin plasma(n=5)	93-98%	99-105%	101-107%	96-105%

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-99%	94-98%	95-100%	100-105%
EDTA plasma(n=5)	91-95%	93-99%	89-94%	96-100%
heparin plasma(n=5)	93-96%	99-106%	97-100%	94-102%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-102%	95-101%	98-104%	97-105%
EDTA plasma(n=5)	96-99%	97-104%	100-105%	89-94%
heparin plasma(n=5)	91-96%	95-100%	99-107%	101-109%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Citations

[1].

"Aging is Associated with Impaired Renal Function, INF-gamma Induced Inflammation and with Alterations in Iron Regulatory Proteins Gene Expression"

E Costa, J Fernandes, S Ribeiro, et al.

From:Aging and Disease

[2].

"The effects of vitamin C on DDP-induced anemia in rats"

LP Gao, Z Li, ZY Guo, et al.

From:Toxicology mechanisms and methods

[3].

"Hemopoietic effect of extracts from constituent herbal medicines of Samul-tang on phenylhydrazine-induced hemolytic anemia in rats"

HW Lee, H Kim, JA Ryuk, et al.

From:International journal of clinical and experimental pathology

[4].

"Down-Regulation of Cardiac Erythropoietin Receptor and its Downstream Activated Signal Transducer Phospho-STAT-5 in a Rat Model of Chronic Kidney ..."

MsC E H B, Rami Barashi M D, Keren G.

From:israel medical association journal

References

1.

"Nucleotide sequence of rat erythropoietin."
Nagao M. , Suga H. , Okano M. , Masuda S. , Narita H. , Ikura K. , Sasaki R.
Biochim. Biophys. Acta1171:99-102(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
strain: Wistar.
tissue: Kidney.
2.

"Erythropoietin structure-function relationships: high degree of sequence homology among mammals."
Wen D. , Boissel J.-P.R. , Tracy T.E. , Gruninger R.H. , Mulcahy L.S. , Czelusniak J. , Goodman M. , Bunn H.F.
Blood82:1507-1516(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-192
strain: Sprague-Dawley.
tissue: Kidney.

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