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Index > Protein center > PDGFA(Gene name) > Bovine
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  • PDGFA (Gene name),
  • Platelet-derived growth factor subunit A (Protein name ),  PDGFA_BOVIN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Gene name:
    PDGFA;
    Protein name:
    Platelet-derived growth factor subunit A(PDGF subunit A);
    Alternative:
    Platelet-derived growth factor alpha polypeptide;Platelet-derived growth factor A chain;
    Organism:
    Bovine (Bos taurus). 
    General Annotation
    Sub Unit:
    Antiparallel disulfide-linked dimer of non-identical (A and B) chains. Homodimers of A and B chains are implicated in transformation processes. Interacts with CSPG4.
    Function:
    Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound.
    Subcellular Location:
    Secreted
    Protein Attributes:
    Sequence length:
    211
    Sequence:
    50:
    MRTWACLLLL | GCGYLANALA | EEAEIPREVI | ERLAHSQIHS | IRDLQRLLEI | 
    100:
    DSVGAEEPLE | TSLRAHGGHG | AKHALEKRPV | PIRRKRSIEE | AIPAVCKTRT | 
    150:
    VIYEIPRSQV | DPTSANFLIW | PPCVEVKRCT | GCCNTSSVKC | QPSRVHHRNV | 
    200:
    KVAKVEYFRK | KAKLKEVQVR | LEEHLECTCT | SASPSPDHRE | EEAGRRRESG | 
    211:
    KKRKRKRLKP | T
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
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    Related Databases
    UniGene:
    Pfam:
    String:
    Uniprot:
     
    FOR
    ELISA Kit for Bovine PDGF subunit A
    MSDS:
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    ELISA Kit for Bovine PDGF subunit A
    Cat.:
    E0112h
    Price:
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    MSDS:
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    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Bovine PDGF subunit A
    Cat.:
    E0112m
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Bovine PDGF subunit A
    Cat.:
    E0112b
    Price:
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    MSDS:
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    Packing:
    96T
    ELISA Kit for Bovine PDGF subunit A
    Cat.:
    E0112r
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Bovine PDGF subunit A
    Cat.:
    U0112r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Bovine PDGF subunit A
    Cat.:
    U0112m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Bovine PDGF subunit A
    Cat.:
    U0112b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Bovine PDGF subunit A
    Cat.:
    U0112h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Bovine PDGF subunit A
    MSDS:
    Please sign in first.
    Polyclonal Antibody for Bovine PDGF subunit A
    Polyclonal Antibody for Bovine PDGF subunit A
    Cat.:
    P0112Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Bovine PDGF subunit A
    Cat.:
    P0112Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Bovine PDGF subunit A
    Polyclonal Antibody for Bovine PDGF subunit A
    Cat.:
    P0112Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Bovine PDGF subunit A
    Monoclonal Antibody for Bovine PDGF subunit A
    Monoclonal Antibody for Bovine PDGF subunit A
    Monoclonal Antibody for Bovine PDGF subunit A
    Monoclonal Antibody for Bovine PDGF subunit A
    Protein for Bovine PDGF subunit A
    Protein for Bovine PDGF subunit A
    Protein for Bovine PDGF subunit A
    Protein for Bovine PDGF subunit A
    Protein for Bovine PDGF subunit A

    R&D Technical Data
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    s

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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤6.8%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤11%

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    Recovery
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    Recovery was determined by spiking various levels of PDGFA into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    102

    99-108

    Plasma

    101

    96-103

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDGFA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    98-105%

    97-107%

    95-108%

    99-107%

    EDTA plasma(n=5)

    97-106%

    98-105%

    98-105%

    96-106%

    heparin plasma(n=5)

    99-105%

     

    100-104%

    96-107%

    95-107%

     

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    References
    1. 1.
      NIH - Mammalian Gene Collection (MGC) project
      Submitted (2005-09) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      strain: Hereford.
      tissue: Ascending colon.
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