KARS ELISA Kit for Human(Homo sapiens) | EIAab.comKARS ELISA Kit for Human(Homo sapiens)

Search Protein-oriented Product center.

For antibodies, ELISA/CLIA, proteins... Product center

Tips

Index > Protein center > KARS(Gene name) > Human

KARS (Gene name),

Lysyl-tRNA synthetase (Protein name ), SYK_HUMAN from NCBI database.

top

General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

Feedback

Gene name:

KARS(KIAA0070);

Protein name:

Lysyl-tRNA synthetase;

Alternative:

Lysine--tRNA ligase(LysRS);

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Homodimer; also part of a multisubunit complex that groups AIMP1, AIMP2, EEF1A1 and tRNA ligases for Arg, Asp, Glu, Gln, Ile, Leu, Lys, Met and Pro. Interacts with AIMP2 (via N-terminus) and MITF. Interacts directly with HIV-1 virus GAG protein.

Function:

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation.

Subcellular Location:

Isoform Mitochondrial Mitochondrion

Protein Attributes:

Sequence length:

597

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

597:

IDRVAMFLTD | SNNIKEVLLF | PAMKPEDKKE | NVATTDTLES | TTVGTSV

3D Structure:

N/A

Predicted Eptitope:

Please Sign in.

Vaild Sequence:

Please Sign in.

Related Databases

KEGG:

hsa:3735

STRING:

9606.ENSP00000325448

String:

Q15046

UniGene:

Hs.3100

SMR:

Q15046

MIM:

601421

Pfam:

PF00152

Uniprot:

Q15046

FOR

ELISA Kit for Human Lysyl-tRNA synthetase

Cat.:

E0187h

Price:

Please sign in first.

Manual:

Looking for manual.

MSDS:

Please sign in first.

Packing:

96T

Range:

Please sign in first.

ELISA Kit for Human Lysyl-tRNA synthetase

Cat.:

E0187m

Price:

Please sign in first.

MSDS:

Please sign in first.

Packing:

96T

CLIA Kit for Human Lysyl-tRNA synthetase

Cat.:

U0187h

Price:

Please sign in first.

MSDS:

Please sign in first.

Packing:

96T

CLIA Kit for Human Lysyl-tRNA synthetase

Cat.:

U0187m

Price:

Please sign in first.

MSDS:

Please sign in first.

Packing:

96T

Polyclonal Antibody for Human Lysyl-tRNA synthetase

Cat.:

P0187Rb-m

Price:

Please sign in first.

Packing:

40ug/0.2ml

Polyclonal Antibody for Human Lysyl-tRNA synthetase

Cat.:

P0187Rb-h

Price:

Please sign in first.

Packing:

40ug/0.2ml

Monoclonal Antibody for Human Lysyl-tRNA synthetase

Protein for Human Lysyl-tRNA synthetase

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Human lysyl-tRNA synthetase accepts nucleotide 73 variants and rescues Escherichia coli double-defective mutant."
Shiba K. , Stello T. , Motegi H. , Noda T. , Musier-Forsyth K. , Schimmel P.
J. Biol. Chem.272:22809-22816(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM CYTOPLASMIC)
tissue: Brain.
2.

"The human lysyl-tRNA synthetase gene encodes both the cytoplasmic and mitochondrial enzymes by means of an unusual alternative splicing of the primary transcript."
Tolkunova E. , Park H. , Xia J. , King M.P. , Davidson E.
J. Biol. Chem.275:35063-35069(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM MITOCHONDRIAL);SUBCELLULAR LOCATION;VARIANT SER-595
3.

"Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1."
Nomura N. , Nagase T. , Miyajima N. , Sazuka T. , Tanaka A. , Sato S. , Seki N. , Kawarabayasi Y. , Ishikawa K. , Tabata S.
DNA Res.1:223-229(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM CYTOPLASMIC);VARIANT SER-595
tissue: Bone marrow.
4.

"The sequence and analysis of duplication-rich human chromosome 16."
Martin J. , Han C. , Gordon L.A. , Terry A. , Prabhakar S. , She X. , Xie G. , Hellsten U. , Chan Y.M. , Altherr M. , Couronne O. , Aerts A. , Bajorek E. , Black S. , Blumer H. , Branscomb E. , Brown N.C. , Bruno W.J. , more...
Nature432:988-994(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
5.

Mural R.J. , Istrail S. , Sutton G.G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-09) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
6.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM CYTOPLASMIC)
tissue: Placenta.
7.

Bienvenut W.V. , Boldt K. , von Kriegsheim A.F. , Kolch W.
Submitted (2007-07) to the UniProtKB

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 2-10; 112-127; 142-148; 231-241; 306-314 AND 486-492;CLEAVAGE OF INITIATOR METHIONINE;ACETYLATION AT ALA-2;IDENTIFICATION BY MASS SPECTROMETRY (ISOFORM CYTOPLASMIC)
tissue: Hepatoma.
8.

"Enzymatic synthesis of diadenosine tetraphosphate and diadenosine triphosphate with a purified lysyl-tRNA synthetase."
Zamecnik P.C. , Stephenson M.L. , Janeway C.M. , Randerath K.
Biochem. Biophys. Res. Commun.24:91-97(1966) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
9.

"Macromolecular assemblage of aminoacyl-tRNA synthetases: identification of protein-protein interactions and characterization of a core protein."
Quevillon S. , Robinson J.-C. , Berthonneau E. , Siatecka M. , Mirande M.
J. Mol. Biol.285:183-195(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH AIMP2
10.

"The telomeric protein Rap1 is conserved in vertebrates and is expressed from a bidirectional promoter positioned between the Rap1 and KARS genes."
Tan M. , Wei C. , Price C.M.
Gene323:1-10(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: BIDIRECTIONAL PROMOTER WITH TERF2IP
11.

"The function of lysyl-tRNA synthetase and Ap4A as signaling regulators of MITF activity in FcepsilonRI-activated mast cells."
Lee Y.N. , Nechushtan H. , Figov N. , Razin E.
Immunity20:145-151(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH MIFT
12.

"The interaction between HIV-1 Gag and human lysyl-tRNA synthetase during viral assembly."
Javanbakht H. , Halwani R. , Cen S. , Saadatmand J. , Musier-Forsyth K. , Gottlinger H. , Kleiman L.
J. Biol. Chem.278:27644-27651(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBUNIT;INTERACTION WITH HIV-1 GAG
13.

"Cellular distribution of Lysyl-tRNA synthetase and its interaction with Gag during human immunodeficiency virus type 1 assembly."
Halwani R. , Cen S. , Javanbakht H. , Saadatmand J. , Kim S. , Shiba K. , Kleiman L.
J. Virol.78:7553-7564(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;MUTAGENESIS OF 1-MET--GLY-65;INTERACTION WITH AIMP2 AND HIV-1 GAG
14.

"Human lysyl-tRNA synthetase is secreted to trigger proinflammatory response."
Park S.G. , Kim H.J. , Min Y.H. , Choi E.-C. , Shin Y.K. , Park B.-J. , Lee S.W. , Kim S.
Proc. Natl. Acad. Sci. U.S.A.102:6356-6361(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;FUNCTION
15.

"Lysyl-tRNA synthetase interacts with EF1alpha, aspartyl-tRNA synthetase and p38 in vitro."
Guzzo C.M. , Yang D.C.H.
Biochem. Biophys. Res. Commun.365:718-723(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH EEF1A1; AIMP2 AND DARS
16.

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S. , Helbig A.O. , Slijper M. , Krijgsveld J. , Heck A.J. , Mohammed S.
Anal. Chem.81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
17.

"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C. , Kumar C. , Gnad F. , Nielsen M.L. , Rehman M. , Walther T.C. , Olsen J.V. , Mann M.
Science325:834-840(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-88 AND LYS-141;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
18.

"Initial characterization of the human central proteome."
Burkard T.R. , Planyavsky M. , Kaupe I. , Breitwieser F.P. , Buerckstuemmer T. , Bennett K.L. , Superti-Furga G. , Colinge J.
BMC Syst. Biol.5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
19.

"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V. , Sumpton D. , Martinez A. , Lilla S. , Espagne C. , Meinnel T. , Giglione C.
Mol. Cell. Proteomics11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
20.

"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P. , Lasa M. , Polevoda B. , Gazquez C. , Elosegui-Artola A. , Kim D.S. , De Juan-Pardo E. , Demeyer K. , Hole K. , Larrea E. , Timmerman E. , Prieto J. , Arnesen T. , Sherman F. , Gevaert K. , Aldabe R.
Proc. Natl. Acad. Sci. U.S.A.109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
21.

"Crystal structure of tetrameric form of human lysyl-tRNA synthetase: Implications for multisynthetase complex formation."
Guo M. , Ignatov M. , Musier-Forsyth K. , Schimmel P. , Yang X.-L.
Proc. Natl. Acad. Sci. U.S.A.105:2331-2336(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.31 ANGSTROMS) OF 70-581 IN COMPLEX WITH SUBSTRATE AND ATP
22.

"Compound heterozygosity for loss-of-function lysyl-tRNA synthetase mutations in a patient with peripheral neuropathy."
McLaughlin H.M. , Sakaguchi R. , Liu C. , Igarashi T. , Pehlivan D. , Chu K. , Iyer R. , Cruz P. , Cherukuri P.F. , Hansen N.F. , Mullikin J.C. , Biesecker L.G. , Wilson T.E. , Ionasescu V. , Nicholson G. , Searby C. , Talbot K. , Vance J.M. , more...
Am. J. Hum. Genet.87:560-566(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS CMTRIB HIS-105 AND MET-274;VARIANT SER-595
23.

"Mutations in KARS, encoding lysyl-tRNA synthetase, cause autosomal-recessive nonsyndromic hearing impairment DFNB89."
University of Washington Center for Mendelian Genomics , Santos-Cortez R.L. , Lee K. , Azeem Z. , Antonellis P.J. , Pollock L.M. , Khan S. , Ullah I. , Andrade-Elizondo P.B. , Chiu I. , Adams M.D. , Basit S. , Smith J.D. , Nickerson D.A. , McDermott B.M. Jr. , Ahmad W. , Leal S.M.
Am. J. Hum. Genet.93:132-140(2013) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS DFNB89 HIS-145 AND ASN-349

Product Feedback Wall

* Email:
*Comments: