Vascular endothelial growth factor A (Protein name
), VEGFA_HUMAN from NCBI database.
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General Annotation
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Antigen Annotation
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3D
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Predicted Eptitope
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Vaild Sequence
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Related Databases
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Citations (4)
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Gene name:
VEGFA(VEGF);
Protein name:
Vascular endothelial growth factor A(VEGF-A);
Alternative:
Vascular permeability factor(VPF);
Organism:
Human (Homo sapiens).
General Annotation
Sub Unit:
Homodimer; disulfide-linked. Also found as heterodimer with PGF.
Function:
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
Subcellular Location:
Secreted
VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤ 4.8%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤ 10.2%
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤7.5%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤11.7%
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤3.9%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.7%
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Recovery
Recovery was determined by spiking various levels of VEGF-A into serum and plasma .
Sample Type
Average(%)
Recovery Range (%)
Serum
86
80-95
Plasma
95
90-102
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Recovery was determined by spiking various levels of VEGFA into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
103
99-106
Plasma
102
96-107
Recovery was determined by spiking various levels of ACE into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
99
91-103
Plasma
98
93-106
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Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VEGF-A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
84-93%
79-94%
88-96%
78-91%
EDTA plasma(n=5)
78-89%
82-97%
90-102%
89-103%
heparin plasma(n=5)
83-94%
89-101%
87-99%
91-103%
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VEGFA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
98-107%
101-104%
99-106%
100-106%
EDTA plasma(n=5)
100-105%
100-108%
100-105%
99-107%
heparin plasma(n=5)
97-109%
99-106%
95-110%
100-105%
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
92-105%
90-101%
95-105%
92-109%
EDTA plasma(n=5)
89-95%
93105%
89-94%
90-106%
heparin plasma(n=5)
93-104%
94-110%
101-111%
91-110%
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Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS VEGF189 AND VEGF165)
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Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM VEGF189);PARTIAL PROTEIN SEQUENCE
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Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM VEGF189)
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Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM VEGF206)
"Human cDNA for vascular endothelial growth factor isoform VEGF121." Sato J.D.
,
Whitney R.G.
Submitted (1999-12) to the EMBL/GenBank/DDBJ databases
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Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM VEGF121)
13.
"Cloning of vascular endothelial growth factor (VEGF) cDNA." Liu J.
,
Peng X.
,
Yuan J.
,
Qiang B.
Submitted (2001-07) to the EMBL/GenBank/DDBJ databases
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Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM VEGF165)
"Cloning and characterization of VEGF from LNCaP cells, a line of prostate cancer cells." Koul S.
,
Johnson T.
,
Meacham R.B.
,
Koul H.K.
Submitted (2004-09) to the EMBL/GenBank/DDBJ databases
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Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM VEGF165)
16.
"VEGF111, a new VEGF-A variant lacking exons 5, 6 and 7." Mineur P.J.
,
Colige A.C.
,
Lambert C.A.
Submitted (2005-10) to the EMBL/GenBank/DDBJ databases
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Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM VEGF111)
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Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM VEGF165)
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Cited for: PARTIAL NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM L-VEGF121)
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Cited for: PRELIMINARY PROTEIN SEQUENCE OF 27-36; 43-50 AND 59-81
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Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 114-209 (ISOFORM VEGF183)
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Cited for: ALTERNATIVE SPLICING (ISOFORMS L-VEGF121 AND L-VEGF165);PROCESSING;SUBCELLULAR LOCATION
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Cited for: ALTERNATIVE SPLICING (ISOFORM L-VEGF189);PROCESSING;SUBCELLULAR LOCATION
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Cited for: INVOLVEMENT IN MICROVASCULAR COMPLICATIONS OF DIABETES
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Cited for: ALTERNATIVE SPLICING (ISOFORMS L-VEGF121; L-VEGF165 AND L-VEGF189)
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: ALTERNATIVE SPLICING (ISOFORMS L-VEGF121 AND L-VEGF165);PROCESSING;SUBCELLULAR LOCATION
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Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 34-135
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Cited for: X-RAY CRYSTALLOGRAPHY (1.93 ANGSTROMS) OF 34-135
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Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 39-134