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Index > Protein center > HIS(Shorten name) > General
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  • General Annotation
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  • Shorten name:
    HIS;
    Antigen name:
    Histamine;
    Alternative:

    General Annotation
    Sub Unit:
    Function:
    Histamine is an organic nitrogen compound involved in local immune responses as well as regulating physiological function in the gut and acting as a neurotransmitter. Histamine triggers the inflammatory response.
    Location:
    As part of an immune response to foreign pathogens, histamine is produced by basophils and by mast cells found in nearby connective tissues. Histamine increases the permeability of the capillaries to white blood cells and some proteins, to allow them to engage pathogens in the infected tissues.
    Antigen Attributes:
    Formula:
    C5H9N3
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
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    FOR
    ELISA Kit for General Histamine
    Cat.:
    E0927Ge
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for General Histamine
    Cat.:
    U0927Ge
    Price:
    Please sign in first.
    MSDS:
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    Packing:
    96T
    Range:
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    R&D Technical Data
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤8%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤11%

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Recovery

    Recovery was determined by spiking various levels of Histamine into serum and plasma .

    Sample Type

    Average(%)

    Recovery  Range (%)

    Serum

    92

    89-101

    Plasma

    87

    82-96

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Linearity

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Histamine and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    80-98%          

    84-101%

    86-99%

    94-105%

    EDTA plasma(n=5)

    88-98%

    87-101%

    79-103%

    90-99%

    heparin plasma(n=5)

    82-93%

     

    84-97%

    93-104%

    91-103%

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    References
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